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Mol. Cells 2008; 26(5): 454-458

Published online January 1, 1970

© The Korean Society for Molecular and Cellular Biology

Estrogen Receptor-alpha Mediates the Effects of Estradiol on Telomerase Activity in Human Mesenchymal Stem Cells

Young Cha, Su Jin Kwon, Wongi Seol and Kyung-Soon Park

Abstract

Sex steroid hormone receptors play a central role in modulating telomerase activity, especially in cancer cells. However, information on the regulation of steroid hormone receptors and their distinct functions on telomerase activity within the mesenchymal stem cell are largely unavailable due to low telomerase activity in the cell. In this study, the effects of estrogen (E(2)) treatment and function of estrogen receptor alpha (ER alpha) and estrogen receptor beta (ER beta) on telomerase activity were investigated in human mesenchymal stem cells (hMSCs). Telomerase activity and mRNA expression of the catalytic subunit of telomerase (hTERT) were upregulated by treatment of the cells with E2. The protein concentration of ER alpha was also increased by E(2) treatment, and enhancement of ER alpha accumulation in the nucleus was clearly detected with immunocytochemistry. When ER alpha expression was reduced by siRNA transfection into hMSCs, the effect of E(2) on the induction of hTERT expression and telomerase activity was diminished. In contrast, the transient overexpression of ER alpha increased the effect of E(2) on the expression of hTERT mRNA. These findings indicate that the activation of hTERT expression and telomerase activity by E(2) in hMSCs depends on ER alpha, but not on ER beta.

Keywords AKT1, estrogen receptor, mesenchymal stem cells, telomerase

Article

Research Article

Mol. Cells 2008; 26(5): 454-458

Published online November 30, 2008

Copyright © The Korean Society for Molecular and Cellular Biology.

Estrogen Receptor-alpha Mediates the Effects of Estradiol on Telomerase Activity in Human Mesenchymal Stem Cells

Young Cha, Su Jin Kwon, Wongi Seol and Kyung-Soon Park

Abstract

Sex steroid hormone receptors play a central role in modulating telomerase activity, especially in cancer cells. However, information on the regulation of steroid hormone receptors and their distinct functions on telomerase activity within the mesenchymal stem cell are largely unavailable due to low telomerase activity in the cell. In this study, the effects of estrogen (E(2)) treatment and function of estrogen receptor alpha (ER alpha) and estrogen receptor beta (ER beta) on telomerase activity were investigated in human mesenchymal stem cells (hMSCs). Telomerase activity and mRNA expression of the catalytic subunit of telomerase (hTERT) were upregulated by treatment of the cells with E2. The protein concentration of ER alpha was also increased by E(2) treatment, and enhancement of ER alpha accumulation in the nucleus was clearly detected with immunocytochemistry. When ER alpha expression was reduced by siRNA transfection into hMSCs, the effect of E(2) on the induction of hTERT expression and telomerase activity was diminished. In contrast, the transient overexpression of ER alpha increased the effect of E(2) on the expression of hTERT mRNA. These findings indicate that the activation of hTERT expression and telomerase activity by E(2) in hMSCs depends on ER alpha, but not on ER beta.

Keywords: AKT1, estrogen receptor, mesenchymal stem cells, telomerase

Mol. Cells
May 31, 2023 Vol.46 No.5, pp. 259~328
COVER PICTURE
The alpha-helices in the lamin filaments are depicted as coils, with different subdomains distinguished by various colors. Coil 1a is represented by magenta, coil 1b by yellow, L2 by green, coil 2a by white, coil 2b by brown, stutter by cyan, coil 2c by dark blue, and the lamin Ig-like domain by grey. In the background, cells are displayed, with the cytosol depicted in green and the nucleus in blue (Ahn et al., pp. 309-318).

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