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Mol. Cells 2007; 23(1): 88-93

Published online February 28, 2007

© The Korean Society for Molecular and Cellular Biology

Short-Term Cold Exposure May Cause a Local Decrease of Neuropeptide Y in the Rat Hypothalamus

Joong Jean Park, Heung Kyung Lee, Min Woo Shin, Sung Jin Kim, Seung Yeon Noh, Jin Shin, Woo Sung Yu

Abstract

Neuropeptide Y (NPY) is an orexigenic and hypothermic peptide. To understand its role in hypothermic conditions, male rats were placed in a 24?C or 4?C air chamber for 1.5 h. The expression of c-Fos protein, and NPY mRNA and protein, was analyzed in the hypothalamus 1 h?2 h later. The cold treatment increased the number of c-Fos-immunoreactive cells in the paraventricular hypothalamic nucleus (PVN) and arcuate nucleus (ARC). At the same time it decreased the density of NPY-immunoreactive components in the PVN, dorsomedial hypothalamic nucleus and ARC, as well as of NPY transcripts in the PVN and ARC. No colocalization of c-Fos with NPY was detected. These results suggest that short-term cold exposure should reduce indirectly NPY production in some hypothalamic nuclei to facilitate thermogenesis without inducing feeding behavior.

Keywords Arcuate Nucleus; c-Fos; Hypothalamus; Hypothermia; Neuropeptide Y.

Article

Research Article

Mol. Cells 2007; 23(1): 88-93

Published online February 28, 2007

Copyright © The Korean Society for Molecular and Cellular Biology.

Short-Term Cold Exposure May Cause a Local Decrease of Neuropeptide Y in the Rat Hypothalamus

Joong Jean Park, Heung Kyung Lee, Min Woo Shin, Sung Jin Kim, Seung Yeon Noh, Jin Shin, Woo Sung Yu

Abstract

Neuropeptide Y (NPY) is an orexigenic and hypothermic peptide. To understand its role in hypothermic conditions, male rats were placed in a 24?C or 4?C air chamber for 1.5 h. The expression of c-Fos protein, and NPY mRNA and protein, was analyzed in the hypothalamus 1 h?2 h later. The cold treatment increased the number of c-Fos-immunoreactive cells in the paraventricular hypothalamic nucleus (PVN) and arcuate nucleus (ARC). At the same time it decreased the density of NPY-immunoreactive components in the PVN, dorsomedial hypothalamic nucleus and ARC, as well as of NPY transcripts in the PVN and ARC. No colocalization of c-Fos with NPY was detected. These results suggest that short-term cold exposure should reduce indirectly NPY production in some hypothalamic nuclei to facilitate thermogenesis without inducing feeding behavior.

Keywords: Arcuate Nucleus, c-Fos, Hypothalamus, Hypothermia, Neuropeptide Y.

Mol. Cells
Aug 31, 2022 Vol.45 No.8, pp. 513~602
COVER PICTURE
Cryo-EM structure of human porphyrin transporter ABCB6 (main figure) shows that binding of hemin (inset, magenta) in concert with two glutathione molecules (cyan) primes ABCB6 for high ATP turnover (Kim et al., pp. 575-587).

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