Mol. Cells 2007; 23(2): 198-206
Published online January 1, 1970
© The Korean Society for Molecular and Cellular Biology
Hydroquinone is a toxic compound and a major benzene metabolite. We report that it strongly inhibits the activation of macrophages and associated cells. Thus, it suppressed the production of proinflammatory cytokines [tumor necrosis factor (TNF)-?, interleukin (IL)-1?, IL-3, IL-6, IL-10, IL-12p40, IL-23], secretion of toxic molecules [nitric oxide (NO) and reactive oxygen species (ROS)] and the activation and expression of CD29 as judged by cell-cell adhesion and surface staining experiments. The inhibition was due to the induction of heme oxygenase (HO)-1 in LPS-activated macrophages, since blocking HO-1 activity with ZnPP, an HO-1 specific inhibitor, abolished hydroquinone¡?s NO inhibitory activity. In addition, hydroquinone and inhibitors (wortmannin and LY294002) of the phosphatidylinositol-3 kinase (PI3K)/Akt pathway had very similar inhibitory effects on LPS-induced and CD29-mediated macrophage responses, including the pho-shorylation of Akt. Therefore, our data suggest that hydroquinone inhibits macrophage-mediated immune re-sponses by modulating intracellular signaling and protective mechanisms.
Keywords Akt; Cell-Cell Adhesion;, Cytokines; Cytotoxic Molecules;, Heme Oxygenase-1; Hydroquinone;, Macrophages
Mol. Cells 2007; 23(2): 198-206
Published online April 30, 2007
Copyright © The Korean Society for Molecular and Cellular Biology.
Ji Yeon Lee, Joo Young Kim, Yong Gyu Lee, Won Cheol Shin, Taehoon Chun, Man Hee Rhee, Jae Youl Cho
Hydroquinone is a toxic compound and a major benzene metabolite. We report that it strongly inhibits the activation of macrophages and associated cells. Thus, it suppressed the production of proinflammatory cytokines [tumor necrosis factor (TNF)-?, interleukin (IL)-1?, IL-3, IL-6, IL-10, IL-12p40, IL-23], secretion of toxic molecules [nitric oxide (NO) and reactive oxygen species (ROS)] and the activation and expression of CD29 as judged by cell-cell adhesion and surface staining experiments. The inhibition was due to the induction of heme oxygenase (HO)-1 in LPS-activated macrophages, since blocking HO-1 activity with ZnPP, an HO-1 specific inhibitor, abolished hydroquinone¡?s NO inhibitory activity. In addition, hydroquinone and inhibitors (wortmannin and LY294002) of the phosphatidylinositol-3 kinase (PI3K)/Akt pathway had very similar inhibitory effects on LPS-induced and CD29-mediated macrophage responses, including the pho-shorylation of Akt. Therefore, our data suggest that hydroquinone inhibits macrophage-mediated immune re-sponses by modulating intracellular signaling and protective mechanisms.
Keywords: Akt, Cell-Cell Adhesion,, Cytokines, Cytotoxic Molecules,, Heme Oxygenase-1, Hydroquinone,, Macrophages
Sin-Ae Lee, Seok Hee Park and Byung-Chul Kim
Mol. Cells 2008; 26(1): 48-52 https://doi.org/10.14348/.2008.26.1.48