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Mol. Cells 2007; 24(1): 9-15

Published online August 31, 2007

© The Korean Society for Molecular and Cellular Biology

Redifferentiation of Dedifferentiated Chondrocytes on Chitosan Membranes and Involvement of PKCα and P38 MAP Kinase

Yoon Ae Lee, Shin-Sung Kang, Suk-Hwan Baek, Jae-Chang Jung, Eun Jung Jin, Eun Nam Tak and Jong Kyung Sonn

Abstract

To investigate the effects of chitosan on the redifferentiation of dedifferentiated chondrocytes, we used chondrocytes obtained from a micromass culture system. Micromass cultures of chick wing bud mesenchymal cells yielded differentiated chondrocytes, but these dedifferentiated during serial monolayer subculture. When the dedifferentiated chondrocytes were cultured on chitosan membranes they regained the phenotype of differentiated chondrocytes. Expression of protein kinase C (PKC) increased during chondrogenesis, decreased during dedifferentiation, and increased again during redifferentiation. Treatment of the cultures with phorbol 12-myristate 13-acetate (PMA) inhibited redifferentiation and down-regulated PKC. In addition, the expression of p38 mitogen-activated protein (MAP) kinase increased during redifferentiation, and its inhibition suppressed redifferentiation. These findings establish a culture system for producing chondrocytes, point to a new role of chitosan in the redifferentiation of dedifferentiated chondrocytes, and show that PKC and p38 MAP kinase activities are required for chondrocyte redifferen-
tiation in this model system.

Keywords Chitosan; Chondrogenesis; Dedifferentiation; p38 MAP Kinase; PKC; Redifferentiation

Article

Research Article

Mol. Cells 2007; 24(1): 9-15

Published online August 31, 2007

Copyright © The Korean Society for Molecular and Cellular Biology.

Redifferentiation of Dedifferentiated Chondrocytes on Chitosan Membranes and Involvement of PKCα and P38 MAP Kinase

Yoon Ae Lee, Shin-Sung Kang, Suk-Hwan Baek, Jae-Chang Jung, Eun Jung Jin, Eun Nam Tak and Jong Kyung Sonn

Abstract

To investigate the effects of chitosan on the redifferentiation of dedifferentiated chondrocytes, we used chondrocytes obtained from a micromass culture system. Micromass cultures of chick wing bud mesenchymal cells yielded differentiated chondrocytes, but these dedifferentiated during serial monolayer subculture. When the dedifferentiated chondrocytes were cultured on chitosan membranes they regained the phenotype of differentiated chondrocytes. Expression of protein kinase C (PKC) increased during chondrogenesis, decreased during dedifferentiation, and increased again during redifferentiation. Treatment of the cultures with phorbol 12-myristate 13-acetate (PMA) inhibited redifferentiation and down-regulated PKC. In addition, the expression of p38 mitogen-activated protein (MAP) kinase increased during redifferentiation, and its inhibition suppressed redifferentiation. These findings establish a culture system for producing chondrocytes, point to a new role of chitosan in the redifferentiation of dedifferentiated chondrocytes, and show that PKC and p38 MAP kinase activities are required for chondrocyte redifferen-
tiation in this model system.

Keywords: Chitosan, Chondrogenesis, Dedifferentiation, p38 MAP Kinase, PKC, Redifferentiation

Mol. Cells
Aug 31, 2022 Vol.45 No.8, pp. 513~602
COVER PICTURE
Cryo-EM structure of human porphyrin transporter ABCB6 (main figure) shows that binding of hemin (inset, magenta) in concert with two glutathione molecules (cyan) primes ABCB6 for high ATP turnover (Kim et al., pp. 575-587).

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