Mol. Cells 2013; 36(6): 542-547
Published online November 27, 2013
https://doi.org/10.1007/s10059-013-0237-0
© The Korean Society for Molecular and Cellular Biology
Tribless family proteins are pseudokinases that lack DFG (Asp-Phe-Gly) motif in the functional kinase domain, regulating Akt and BMP pathways, insulin metabolism, hypoxia, and ubiquitination. This report concerns expression patterns and functional roles of trb3 in zebrafish embryonic development. trb3 is evolutionarily well-conserved and located on zebrafish chromosome 11. Spatiotemporal expression studies show that trb3 transcripts are abundant throughout embryogenesis, but confined to mesendodermal cells during the late blastula phase. Over-expression of trb3 ventralizes the embryos while a knockdown of trb3 using morpholino alters positioning of the heart, liver, and pancreatic buds as well as gut looping. Furthermore, constitutive activation of TGF-β signaling with TARAM-A* (TGF-β-related type I receptor) significantly increases the level of trb3 transcripts during the late blastula phase. Over-expression of trb3 reduces the level of smurf1 transcripts, a member of TGF-β signaling. We thus propose that Trb3 governs left-right (LR) axis patterning as a component of TGF-β signaling in vertebrate embryonic development.
Keywords left-right (LR) axis, mesendodermal cells, TGF-β, Tribless, zebrafish
Mol. Cells 2013; 36(6): 542-547
Published online December 31, 2013 https://doi.org/10.1007/s10059-013-0237-0
Copyright © The Korean Society for Molecular and Cellular Biology.
Mrudhula Anuppalle, Sateesh Maddirevula, Tae-Lin Huh, and Myungchull Rhee
Tribless family proteins are pseudokinases that lack DFG (Asp-Phe-Gly) motif in the functional kinase domain, regulating Akt and BMP pathways, insulin metabolism, hypoxia, and ubiquitination. This report concerns expression patterns and functional roles of trb3 in zebrafish embryonic development. trb3 is evolutionarily well-conserved and located on zebrafish chromosome 11. Spatiotemporal expression studies show that trb3 transcripts are abundant throughout embryogenesis, but confined to mesendodermal cells during the late blastula phase. Over-expression of trb3 ventralizes the embryos while a knockdown of trb3 using morpholino alters positioning of the heart, liver, and pancreatic buds as well as gut looping. Furthermore, constitutive activation of TGF-β signaling with TARAM-A* (TGF-β-related type I receptor) significantly increases the level of trb3 transcripts during the late blastula phase. Over-expression of trb3 reduces the level of smurf1 transcripts, a member of TGF-β signaling. We thus propose that Trb3 governs left-right (LR) axis patterning as a component of TGF-β signaling in vertebrate embryonic development.
Keywords: left-right (LR) axis, mesendodermal cells, TGF-β, Tribless, zebrafish
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