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Mol. Cells 2013; 35(5): 436-443

Published online May 31, 2013

https://doi.org/10.1007/s10059-013-0046-5

© The Korean Society for Molecular and Cellular Biology

The Effect of Amino Density on the Attachment, Migration, and Differentiation of Rat Neural Stem Cells In Vitro

Hai-Long Li, Han Zhang, Hua Huang, Zhen-Qiang Liu, Yan-Bing Li, Hao Yu, and Yi-Hua An

Beijing Neurosurgical Institute, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China

Received: February 8, 2013; Revised: March 18, 2013; Accepted: March 20, 2013

Abstract

Artificial extracellular matrices play important roles in the regulation of stem cell behavior. To generate materials for tissue engineering, active functional groups, such as amino, carboxyl, and hydroxyl, are often introduced to change the properties of the biomaterial surface. In this study, we chemically modified coverslips to create sur-faces with different amino densities and investigated the adhesion, migration, and differentiation of neural stem cells (NSCs) under serum-free culture conditions. We observed that a higher amino density significantly promoted NSCs attach-ment, enhanced neuronal differentiation and promoted excitatory synapse formation in vitro. These results indicate that the amino density significantly affected the biological behavior of NSCs. Thus, the density and impact of functional groups in extracellular matrices should be considered in the research and development of materials for tissue engineering.

Keywords biocompatible, cell adhesion molecular, cell migration assays, coated materials, neural stem cells

Article

Research Article

Mol. Cells 2013; 35(5): 436-443

Published online May 31, 2013 https://doi.org/10.1007/s10059-013-0046-5

Copyright © The Korean Society for Molecular and Cellular Biology.

The Effect of Amino Density on the Attachment, Migration, and Differentiation of Rat Neural Stem Cells In Vitro

Hai-Long Li, Han Zhang, Hua Huang, Zhen-Qiang Liu, Yan-Bing Li, Hao Yu, and Yi-Hua An

Beijing Neurosurgical Institute, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China

Received: February 8, 2013; Revised: March 18, 2013; Accepted: March 20, 2013

Abstract

Artificial extracellular matrices play important roles in the regulation of stem cell behavior. To generate materials for tissue engineering, active functional groups, such as amino, carboxyl, and hydroxyl, are often introduced to change the properties of the biomaterial surface. In this study, we chemically modified coverslips to create sur-faces with different amino densities and investigated the adhesion, migration, and differentiation of neural stem cells (NSCs) under serum-free culture conditions. We observed that a higher amino density significantly promoted NSCs attach-ment, enhanced neuronal differentiation and promoted excitatory synapse formation in vitro. These results indicate that the amino density significantly affected the biological behavior of NSCs. Thus, the density and impact of functional groups in extracellular matrices should be considered in the research and development of materials for tissue engineering.

Keywords: biocompatible, cell adhesion molecular, cell migration assays, coated materials, neural stem cells

Mol. Cells
May 31, 2022 Vol.45 No.5, pp. 273~352
COVER PICTURE
Fe2+ ion depletion-induced expression of BΔGFP at the early stage of leaf development (Choi et al., pp. 294-305).

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