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Mol. Cells 2013; 35(5): 402-409

Published online May 31, 2013

https://doi.org/10.1007/s10059-013-2337-2

© The Korean Society for Molecular and Cellular Biology

OsCpn60alpha1, Encoding the Plastid Chaperonin 60alpha Subunit, Is Essential for Folding of rbcL

Sung-Ryul Kim, Jung-Il Yang, and Gynheung An

Crop Biotech Institute and Department of Genetic Engineering, Kyung Hee University, Yongin 446-701, Korea

Received: December 24, 2013; Revised: March 12, 2013; Accepted: March 12, 2013

Abstract

Chaperonins are involved in protein-folding. The rice genome encodes six plastid chaperonin subunits (Cpn60) - three ? and three ?. Our study showed that they were differentially expressed during normal plant development. Moreover, five were induced by heat stress (42?C) but not by cold (10°C). The oscpn60?1 mutant had a pale-green phenotype at the seedling stage and development ceased after the fourth leaf appeared. Transiently expressed OsCpn60?1:GFP fusion protein was localized to the chloroplast stroma. Immuno-blot analysis indicated that the level of Rubisco large subunit (rbcL) was severely reduced in the mutant while levels were unchanged for some imported proteins, e.g., stromal heat shock protein 70 (Hsp70) and chlorophyll a/b binding protein 1 (Lhcb1). This demonstrated that OsCpn60?1 is required for the folding of rbcL and that failure of that process is seedling-lethal.

Keywords chaperonin, Cpn60, plastid, rice, Rubisco

Article

Research Article

Mol. Cells 2013; 35(5): 402-409

Published online May 31, 2013 https://doi.org/10.1007/s10059-013-2337-2

Copyright © The Korean Society for Molecular and Cellular Biology.

OsCpn60alpha1, Encoding the Plastid Chaperonin 60alpha Subunit, Is Essential for Folding of rbcL

Sung-Ryul Kim, Jung-Il Yang, and Gynheung An

Crop Biotech Institute and Department of Genetic Engineering, Kyung Hee University, Yongin 446-701, Korea

Received: December 24, 2013; Revised: March 12, 2013; Accepted: March 12, 2013

Abstract

Chaperonins are involved in protein-folding. The rice genome encodes six plastid chaperonin subunits (Cpn60) - three ? and three ?. Our study showed that they were differentially expressed during normal plant development. Moreover, five were induced by heat stress (42?C) but not by cold (10°C). The oscpn60?1 mutant had a pale-green phenotype at the seedling stage and development ceased after the fourth leaf appeared. Transiently expressed OsCpn60?1:GFP fusion protein was localized to the chloroplast stroma. Immuno-blot analysis indicated that the level of Rubisco large subunit (rbcL) was severely reduced in the mutant while levels were unchanged for some imported proteins, e.g., stromal heat shock protein 70 (Hsp70) and chlorophyll a/b binding protein 1 (Lhcb1). This demonstrated that OsCpn60?1 is required for the folding of rbcL and that failure of that process is seedling-lethal.

Keywords: chaperonin, Cpn60, plastid, rice, Rubisco

Mol. Cells
Nov 30, 2022 Vol.45 No.11, pp. 763~867
COVER PICTURE
Naive (cyan) and axotomized (magenta) retinal ganglion cell axons in Xenopus tropicalis (Choi et al., pp. 846-854).

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