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Mol. Cells 2013; 35(5): 402-409

Published online April 24, 2013

https://doi.org/10.1007/s10059-013-2337-2

© The Korean Society for Molecular and Cellular Biology

OsCpn60alpha1, Encoding the Plastid Chaperonin 60alpha Subunit, Is Essential for Folding of rbcL

Sung-Ryul Kim, Jung-Il Yang, and Gynheung An

Crop Biotech Institute and Department of Genetic Engineering, Kyung Hee University, Yongin 446-701, Korea

Received: December 24, 2013; Revised: March 12, 2013; Accepted: March 12, 2013

Abstract

Chaperonins are involved in protein-folding. The rice genome encodes six plastid chaperonin subunits (Cpn60) - three ? and three ?. Our study showed that they were differentially expressed during normal plant development. Moreover, five were induced by heat stress (42?C) but not by cold (10°C). The oscpn60?1 mutant had a pale-green phenotype at the seedling stage and development ceased after the fourth leaf appeared. Transiently expressed OsCpn60?1:GFP fusion protein was localized to the chloroplast stroma. Immuno-blot analysis indicated that the level of Rubisco large subunit (rbcL) was severely reduced in the mutant while levels were unchanged for some imported proteins, e.g., stromal heat shock protein 70 (Hsp70) and chlorophyll a/b binding protein 1 (Lhcb1). This demonstrated that OsCpn60?1 is required for the folding of rbcL and that failure of that process is seedling-lethal.

Keywords chaperonin, Cpn60, plastid, rice, Rubisco

Article

Research Article

Mol. Cells 2013; 35(5): 402-409

Published online May 31, 2013 https://doi.org/10.1007/s10059-013-2337-2

Copyright © The Korean Society for Molecular and Cellular Biology.

OsCpn60alpha1, Encoding the Plastid Chaperonin 60alpha Subunit, Is Essential for Folding of rbcL

Sung-Ryul Kim, Jung-Il Yang, and Gynheung An

Crop Biotech Institute and Department of Genetic Engineering, Kyung Hee University, Yongin 446-701, Korea

Received: December 24, 2013; Revised: March 12, 2013; Accepted: March 12, 2013

Abstract

Chaperonins are involved in protein-folding. The rice genome encodes six plastid chaperonin subunits (Cpn60) - three ? and three ?. Our study showed that they were differentially expressed during normal plant development. Moreover, five were induced by heat stress (42?C) but not by cold (10°C). The oscpn60?1 mutant had a pale-green phenotype at the seedling stage and development ceased after the fourth leaf appeared. Transiently expressed OsCpn60?1:GFP fusion protein was localized to the chloroplast stroma. Immuno-blot analysis indicated that the level of Rubisco large subunit (rbcL) was severely reduced in the mutant while levels were unchanged for some imported proteins, e.g., stromal heat shock protein 70 (Hsp70) and chlorophyll a/b binding protein 1 (Lhcb1). This demonstrated that OsCpn60?1 is required for the folding of rbcL and that failure of that process is seedling-lethal.

Keywords: chaperonin, Cpn60, plastid, rice, Rubisco

Mol. Cells
Feb 28, 2023 Vol.46 No.2, pp. 69~129
COVER PICTURE
The bulk tissue is a heterogeneous mixture of various cell types, which is depicted as a skein of intertwined threads with diverse colors each of which represents a unique cell type. Single-cell omics analysis untangles efficiently the skein according to the color by providing information of molecules at individual cells and interpretation of such information based on different cell types. The molecules that can be profiled at the individual cell by single-cell omics analysis includes DNA (bottom middle), RNA (bottom right), and protein (bottom left). This special issue reviews single-cell technologies and computational methods that have been developed for the single-cell omics analysis and how they have been applied to improve our understanding of the underlying mechanisms of biological and pathological phenomena at the single-cell level.

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