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Mol. Cells 2013; 35(6): 481-488

Published online June 30, 2013

https://doi.org/10.1007/s10059-013-2130-2

© The Korean Society for Molecular and Cellular Biology

Requirement of Vesicle-Associated Membrane Protein 721 and 722 for Sustained Growth during Immune Responses in Arabidopsis

Hye Sup Yun, Mark Kwaaitaal, Naohiro Kato, Changhyun Yi, Sohyeon Park, Masa H. Sato, Paul Schulze-Lefert, and Chian Kwon

1Max-Planck-Institut f?r Z?chtungsforschung, Deparment of Plant Microbe Interactions, D-50829, K?ln, Germany, 2Biological Sciences Major, College of Biological Science and Biotechnology, Konkuk University, Seoul 143-701, Korea, 3Department of Biological Sciences, Louisiana State University, LA 70803-1715, USA, 4Department of Molecular Biology, Dankook University, Yongin 448-701, Korea, 5Laboratory of Cellular Dynamics, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto 606-8522, Japan, 6Department of Integrated Molecular Sciences, Dankook University, Yongin 448-701, Korea, 7Present address: Department of Agriculture and Ecology Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark

Received: April 30, 2013; Revised: March 21, 2013; Accepted: March 28, 2013

Abstract

Extracellular immune responses to ascomycete and oomycete pathogens in Arabidopsis are dependent on vesicle-associated secretion mediated by the SNARE proteins PEN1 syntaxin, SNAP33 and endomembrane-resident VAMP721/722. Continuous movement of functional GFPVAMP722 to and from the plasma membrane in nonstimulated cells reflects the second proposed function of VAMP721/722 in constitutive secretion during plant growth and development. Application of the bacterium-derived elicitor flg22 stabilizes VAMP721/722 that are otherwise constitutively degraded via the 26S proteasome pathway. Depletion of VAMP721/722 levels by reducing VAMP721/722 gene dosage enhances flg22-induced seedling growth inhibition in spite of elevated VAMP721/722 abundance. We therefore propose that plants prioritize the deployment of the corresponding secretory pathway for defense over plant growth. Interstingly, VAMP721/722 specifically interact in vitro and in vivo with the plasma membrane syntaxin SYP132 that is required for plant growth and resistance to bacteria. This suggests that the plant growth/immunityinvolved VAMP721/722 form SNARE complexes with multiple plasma membrane syntaxins to discharge cuedependent cargo molecules.

Keywords plant growth, plant immune responses, PM syntaxins, secretion, VAMP721/722

Article

Research Article

Mol. Cells 2013; 35(6): 481-488

Published online June 30, 2013 https://doi.org/10.1007/s10059-013-2130-2

Copyright © The Korean Society for Molecular and Cellular Biology.

Requirement of Vesicle-Associated Membrane Protein 721 and 722 for Sustained Growth during Immune Responses in Arabidopsis

Hye Sup Yun, Mark Kwaaitaal, Naohiro Kato, Changhyun Yi, Sohyeon Park, Masa H. Sato, Paul Schulze-Lefert, and Chian Kwon

1Max-Planck-Institut f?r Z?chtungsforschung, Deparment of Plant Microbe Interactions, D-50829, K?ln, Germany, 2Biological Sciences Major, College of Biological Science and Biotechnology, Konkuk University, Seoul 143-701, Korea, 3Department of Biological Sciences, Louisiana State University, LA 70803-1715, USA, 4Department of Molecular Biology, Dankook University, Yongin 448-701, Korea, 5Laboratory of Cellular Dynamics, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto 606-8522, Japan, 6Department of Integrated Molecular Sciences, Dankook University, Yongin 448-701, Korea, 7Present address: Department of Agriculture and Ecology Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark

Received: April 30, 2013; Revised: March 21, 2013; Accepted: March 28, 2013

Abstract

Extracellular immune responses to ascomycete and oomycete pathogens in Arabidopsis are dependent on vesicle-associated secretion mediated by the SNARE proteins PEN1 syntaxin, SNAP33 and endomembrane-resident VAMP721/722. Continuous movement of functional GFPVAMP722 to and from the plasma membrane in nonstimulated cells reflects the second proposed function of VAMP721/722 in constitutive secretion during plant growth and development. Application of the bacterium-derived elicitor flg22 stabilizes VAMP721/722 that are otherwise constitutively degraded via the 26S proteasome pathway. Depletion of VAMP721/722 levels by reducing VAMP721/722 gene dosage enhances flg22-induced seedling growth inhibition in spite of elevated VAMP721/722 abundance. We therefore propose that plants prioritize the deployment of the corresponding secretory pathway for defense over plant growth. Interstingly, VAMP721/722 specifically interact in vitro and in vivo with the plasma membrane syntaxin SYP132 that is required for plant growth and resistance to bacteria. This suggests that the plant growth/immunityinvolved VAMP721/722 form SNARE complexes with multiple plasma membrane syntaxins to discharge cuedependent cargo molecules.

Keywords: plant growth, plant immune responses, PM syntaxins, secretion, VAMP721/722

Mol. Cells
Aug 31, 2022 Vol.45 No.8, pp. 513~602
COVER PICTURE
Cryo-EM structure of human porphyrin transporter ABCB6 (main figure) shows that binding of hemin (inset, magenta) in concert with two glutathione molecules (cyan) primes ABCB6 for high ATP turnover (Kim et al., pp. 575-587).

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