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Mol. Cells 2013; 35(2): 166-172

Published online February 28, 2013

https://doi.org/10.1007/s10059-013-2307-8

© The Korean Society for Molecular and Cellular Biology

Mutant-Specific Gene Expression Profiling Identifies SRY-Related HMG Box 11b (SOX11b) as a Novel Regulator of Vascular Development in Zebrafish

Christopher E. Schmitt, Melissa J. Woolls, and Suk-Won Jin

1Yale Cardiovascular Research Center and Section of Cardiovascular Medicine, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, 06511, USA, 2Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA, 3These authors contributed equally to this work.

Received: November 27, 2012; Accepted: December 13, 2012

Abstract

Previous studies have identified two zebrafish mutants, cloche and groom of cloche, which lack the majority of the endothelial lineage at early developmental stages. However, at later stages, these avascular mutant embryos generate rudimentary vessels, indicating that they retain the ability to generate endothelial cells despite this initial lack of endothelial progenitors. To further investigate molecular mechanisms that allow the emergence of the endothelial lineage in these avascular mutant embryos, we analyzed the gene expression profile using mi-croarray analysis on isolated endothelial cells. We find that the expression of the genes characteristic of the mesodermal lineages are substantially elevated in the kdrl+ cells isolated from avascular mutant embryos. Subsequent validation and analyses of the microarray data identifies Sox11b, a zebrafish ortholog of SRY-related HMG box 11 (SOX11), which have not previously implicated in vascular development. We further define the function sox11b during vascular development, and find that Sox11b function is essential for developmental angiogenesis in zebrafish embryos, specifically regulat-ing sprouting angiogenesis. Taken together, our analyses illustrate a complex regulation of endothelial specification and differentiation during vertebrate devel-opment.

Keywords cloche, groom of cloche, sox11b, vascular development, zebrafish

Article

Research Article

Mol. Cells 2013; 35(2): 166-172

Published online February 28, 2013 https://doi.org/10.1007/s10059-013-2307-8

Copyright © The Korean Society for Molecular and Cellular Biology.

Mutant-Specific Gene Expression Profiling Identifies SRY-Related HMG Box 11b (SOX11b) as a Novel Regulator of Vascular Development in Zebrafish

Christopher E. Schmitt, Melissa J. Woolls, and Suk-Won Jin

1Yale Cardiovascular Research Center and Section of Cardiovascular Medicine, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, 06511, USA, 2Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA, 3These authors contributed equally to this work.

Received: November 27, 2012; Accepted: December 13, 2012

Abstract

Previous studies have identified two zebrafish mutants, cloche and groom of cloche, which lack the majority of the endothelial lineage at early developmental stages. However, at later stages, these avascular mutant embryos generate rudimentary vessels, indicating that they retain the ability to generate endothelial cells despite this initial lack of endothelial progenitors. To further investigate molecular mechanisms that allow the emergence of the endothelial lineage in these avascular mutant embryos, we analyzed the gene expression profile using mi-croarray analysis on isolated endothelial cells. We find that the expression of the genes characteristic of the mesodermal lineages are substantially elevated in the kdrl+ cells isolated from avascular mutant embryos. Subsequent validation and analyses of the microarray data identifies Sox11b, a zebrafish ortholog of SRY-related HMG box 11 (SOX11), which have not previously implicated in vascular development. We further define the function sox11b during vascular development, and find that Sox11b function is essential for developmental angiogenesis in zebrafish embryos, specifically regulat-ing sprouting angiogenesis. Taken together, our analyses illustrate a complex regulation of endothelial specification and differentiation during vertebrate devel-opment.

Keywords: cloche, groom of cloche, sox11b, vascular development, zebrafish

Mol. Cells
Aug 31, 2022 Vol.45 No.8, pp. 513~602
COVER PICTURE
Cryo-EM structure of human porphyrin transporter ABCB6 (main figure) shows that binding of hemin (inset, magenta) in concert with two glutathione molecules (cyan) primes ABCB6 for high ATP turnover (Kim et al., pp. 575-587).

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