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Mol. Cells 2013; 35(2): 125-133

Published online February 28, 2013

https://doi.org/10.1007/s10059-013-2245-5

© The Korean Society for Molecular and Cellular Biology

ATHB17 Is a Positive Regulator of Abscisic Acid Response during Early Seedling Growth

Min Young Park, Sung-ah Kim, Sun-ji Lee, and Soo Young Kim

Department of Molecular Biotechnology and Kumho Life Science Laboratory, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Korea

Received: September 14, 2012; Revised: December 19, 2012; Accepted: December 21, 2012

Abstract

We performed activation tagging screen to isolate abscisic acid (ABA) response mutants. One of the mutants, designated ahs10 (ABA-hypersensitive10), exhibited ABA-hyper-sensitive phe- notypes. TAIL-PCR analysis of the mutant revealed that T-DNA was inserted in the promoter region of the Arabidopsis gene, At2g01430, which encodes a homeodomain-leucine zipper protein ATHB17. Subsequent expression analysis indicated that ATHB17 was activated in ahs10. To recapitulate the mutant phenotypes, we prepared ATHB17 OX lines and investigated their phenotypes. The results showed that ATHB17 confers ABA-hypersen-sitivity and drought tolerance. On the contrary, ATHB17 knockout lines were ABA-insensitive and drought-sensi-tive, further demonstrating that ATHB17 is involved in ABA and water-stress responses. Interestingly, the ATHB17 effect on seedling growth in the presence of ABA was observed only during the postgermination seedling establishment stage, suggesting that it functions during a narrow developmental window of early seedling growth.

Keywords abscisic acid (ABA), activation tagging, drought tolerance, HD-Zip protein

Article

Research Article

Mol. Cells 2013; 35(2): 125-133

Published online February 28, 2013 https://doi.org/10.1007/s10059-013-2245-5

Copyright © The Korean Society for Molecular and Cellular Biology.

ATHB17 Is a Positive Regulator of Abscisic Acid Response during Early Seedling Growth

Min Young Park, Sung-ah Kim, Sun-ji Lee, and Soo Young Kim

Department of Molecular Biotechnology and Kumho Life Science Laboratory, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Korea

Received: September 14, 2012; Revised: December 19, 2012; Accepted: December 21, 2012

Abstract

We performed activation tagging screen to isolate abscisic acid (ABA) response mutants. One of the mutants, designated ahs10 (ABA-hypersensitive10), exhibited ABA-hyper-sensitive phe- notypes. TAIL-PCR analysis of the mutant revealed that T-DNA was inserted in the promoter region of the Arabidopsis gene, At2g01430, which encodes a homeodomain-leucine zipper protein ATHB17. Subsequent expression analysis indicated that ATHB17 was activated in ahs10. To recapitulate the mutant phenotypes, we prepared ATHB17 OX lines and investigated their phenotypes. The results showed that ATHB17 confers ABA-hypersen-sitivity and drought tolerance. On the contrary, ATHB17 knockout lines were ABA-insensitive and drought-sensi-tive, further demonstrating that ATHB17 is involved in ABA and water-stress responses. Interestingly, the ATHB17 effect on seedling growth in the presence of ABA was observed only during the postgermination seedling establishment stage, suggesting that it functions during a narrow developmental window of early seedling growth.

Keywords: abscisic acid (ABA), activation tagging, drought tolerance, HD-Zip protein

Mol. Cells
Jan 31, 2023 Vol.46 No.1, pp. 1~67
COVER PICTURE
RNAs form diverse shapes and play multiple functions as central molecules of gene expression. In this special issue on RNA, seven minireviews illustrate how basic concepts and recent RNA biology findings are transformed into new and exciting RNA therapeutics.

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