Mol. Cells 2012; 34(6): 549-554
Published online November 27, 2012
https://doi.org/10.1007/s10059-012-0235-7
© The Korean Society for Molecular and Cellular Biology
Correspondence to : *Correspondence: jaeyong@gnu.ac.kr (JYP); emhwang@kist.re.kr (EMH)
Copine1 is a ubiquitously expressed protein found in various tissues including the brain, but little is known about the physiological function of this protein. Here, we showed that copine1 is involved in neuronal differentiation. Over-expression of copine1 clearly increased neurite outgrowth and expression of Tuj1, a neuronal marker protein, in HiB5 cells. In addition, endogenous copine1 was transiently increased at the early time during neuronal differentiation of HiB5 cells. When the expression of endogenous copine1 was knocked-down by its specific shRNA, PDGF-mediated neurite outgrowth was clearly decreased in HiB5 cells. Furthermore, over-expression of copine1 increased phosphorylation of Akt and copine1-specific shRNA de-creased phosphorylation of Akt during neuronal differen-tiation of HiB5 cells. Interestingly, the phosphorylation level of PI3K, generally known as an upstream protein of Akt, was not changed by copine1 expression. These re-sults suggest that copine1 enhances neuronal differentiation of HiB5 cells not through the PI3K-Akt pathway, but by using another Akt activated signal pathway.
Keywords Akt, copine1, HiB5 cell, neuronal differentiation
Mol. Cells 2012; 34(6): 549-554
Published online December 31, 2012 https://doi.org/10.1007/s10059-012-0235-7
Copyright © The Korean Society for Molecular and Cellular Biology.
Nammi Park1,3, Jae Cheal Yoo1,3, Jiwon Ryu1, Seong-Geun Hong1, Eun Mi Hwang2,* , and Jae-Yong Park1,2,*
1Department of Physiology, Institute of Health Science, and Medical Research Center for Neural Dysfunction, School of Medicine, Gyeongsang National University, Jinju 660-751, Korea, 2Center for Neural Science and WCI Center for Functional Connectomics, Korea Institute of Science and Technology, Seoul 136-791, Korea, 3These authors contributed equally to this work.
Correspondence to:*Correspondence: jaeyong@gnu.ac.kr (JYP); emhwang@kist.re.kr (EMH)
Copine1 is a ubiquitously expressed protein found in various tissues including the brain, but little is known about the physiological function of this protein. Here, we showed that copine1 is involved in neuronal differentiation. Over-expression of copine1 clearly increased neurite outgrowth and expression of Tuj1, a neuronal marker protein, in HiB5 cells. In addition, endogenous copine1 was transiently increased at the early time during neuronal differentiation of HiB5 cells. When the expression of endogenous copine1 was knocked-down by its specific shRNA, PDGF-mediated neurite outgrowth was clearly decreased in HiB5 cells. Furthermore, over-expression of copine1 increased phosphorylation of Akt and copine1-specific shRNA de-creased phosphorylation of Akt during neuronal differen-tiation of HiB5 cells. Interestingly, the phosphorylation level of PI3K, generally known as an upstream protein of Akt, was not changed by copine1 expression. These re-sults suggest that copine1 enhances neuronal differentiation of HiB5 cells not through the PI3K-Akt pathway, but by using another Akt activated signal pathway.
Keywords: Akt, copine1, HiB5 cell, neuronal differentiation
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