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Mol. Cells 2012; 34(6): 549-554

Published online December 31, 2012

https://doi.org/10.1007/s10059-012-0235-7

© The Korean Society for Molecular and Cellular Biology

Copine1 Enhances Neuronal Differentiation of the Hippocampal Progenitor HiB5 Cells

Nammi Park1,3, Jae Cheal Yoo1,3, Jiwon Ryu1, Seong-Geun Hong1, Eun Mi Hwang2,* , and Jae-Yong Park1,2,*

1Department of Physiology, Institute of Health Science, and Medical Research Center for Neural Dysfunction, School of Medicine, Gyeongsang National University, Jinju 660-751, Korea, 2Center for Neural Science and WCI Center for Functional Connectomics, Korea Institute of Science and Technology, Seoul 136-791, Korea, 3These authors contributed equally to this work.

Correspondence to : *Correspondence: jaeyong@gnu.ac.kr (JYP); emhwang@kist.re.kr (EMH)

Received: September 5, 2012; Revised: October 24, 2012; Accepted: October 24, 2012

Abstract

Copine1 is a ubiquitously expressed protein found in various tissues including the brain, but little is known about the physiological function of this protein. Here, we showed that copine1 is involved in neuronal differentiation. Over-expression of copine1 clearly increased neurite outgrowth and expression of Tuj1, a neuronal marker protein, in HiB5 cells. In addition, endogenous copine1 was transiently increased at the early time during neuronal differentiation of HiB5 cells. When the expression of endogenous copine1 was knocked-down by its specific shRNA, PDGF-mediated neurite outgrowth was clearly decreased in HiB5 cells. Furthermore, over-expression of copine1 increased phosphorylation of Akt and copine1-specific shRNA de-creased phosphorylation of Akt during neuronal differen-tiation of HiB5 cells. Interestingly, the phosphorylation level of PI3K, generally known as an upstream protein of Akt, was not changed by copine1 expression. These re-sults suggest that copine1 enhances neuronal differentiation of HiB5 cells not through the PI3K-Akt pathway, but by using another Akt activated signal pathway.

Keywords Akt, copine1, HiB5 cell, neuronal differentiation

Article

Research Article

Mol. Cells 2012; 34(6): 549-554

Published online December 31, 2012 https://doi.org/10.1007/s10059-012-0235-7

Copyright © The Korean Society for Molecular and Cellular Biology.

Copine1 Enhances Neuronal Differentiation of the Hippocampal Progenitor HiB5 Cells

Nammi Park1,3, Jae Cheal Yoo1,3, Jiwon Ryu1, Seong-Geun Hong1, Eun Mi Hwang2,* , and Jae-Yong Park1,2,*

1Department of Physiology, Institute of Health Science, and Medical Research Center for Neural Dysfunction, School of Medicine, Gyeongsang National University, Jinju 660-751, Korea, 2Center for Neural Science and WCI Center for Functional Connectomics, Korea Institute of Science and Technology, Seoul 136-791, Korea, 3These authors contributed equally to this work.

Correspondence to:*Correspondence: jaeyong@gnu.ac.kr (JYP); emhwang@kist.re.kr (EMH)

Received: September 5, 2012; Revised: October 24, 2012; Accepted: October 24, 2012

Abstract

Copine1 is a ubiquitously expressed protein found in various tissues including the brain, but little is known about the physiological function of this protein. Here, we showed that copine1 is involved in neuronal differentiation. Over-expression of copine1 clearly increased neurite outgrowth and expression of Tuj1, a neuronal marker protein, in HiB5 cells. In addition, endogenous copine1 was transiently increased at the early time during neuronal differentiation of HiB5 cells. When the expression of endogenous copine1 was knocked-down by its specific shRNA, PDGF-mediated neurite outgrowth was clearly decreased in HiB5 cells. Furthermore, over-expression of copine1 increased phosphorylation of Akt and copine1-specific shRNA de-creased phosphorylation of Akt during neuronal differen-tiation of HiB5 cells. Interestingly, the phosphorylation level of PI3K, generally known as an upstream protein of Akt, was not changed by copine1 expression. These re-sults suggest that copine1 enhances neuronal differentiation of HiB5 cells not through the PI3K-Akt pathway, but by using another Akt activated signal pathway.

Keywords: Akt, copine1, HiB5 cell, neuronal differentiation

Mol. Cells
Sep 30, 2022 Vol.45 No.9, pp. 603~672
COVER PICTURE
The Target of Rapamycin Complex (TORC) is a central regulatory hub in eukaryotes, which is well conserved in diverse plant species, including tomato (Solanum lycopersicum). Inhibition of TORC genes (SlTOR, SlLST8, and SlRAPTOR) by VIGS (virus-induced gene silencing) results in early fruit ripening in tomato. The red/ orange tomatoes are early-ripened TORC-silenced fruits, while the green tomato is a control fruit. Top, left, control fruit (TRV2-myc); top, right, TRV2-SlLST8; bottom, left, TRV2-SlTOR; bottom, right, TRV2-SlRAPTOR(Choi et al., pp. 660-672).

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