TOP
닫기
Search 닫기
Advanced Search Quick links
· Most Cited · Most Read · On-line First · Archives · Go to e-Submission

Research Article

Split Viewer

Mol. Cells 2013; 35(1): 25-31

Published online December 4, 2012

https://doi.org/10.1007/s10059-013-2159-2

© The Korean Society for Molecular and Cellular Biology

A Competitive Peptide Inhibitor KIDARI Negatively Regulates HFR1 by Forming Nonfunctional Heterodimers in Arabidopsis Photomorphogenesis

Shin-Young Hong, Pil Joon Seo, Jae Yong Ryu, Shin-Hae Cho, Je-Chang Woo, and Chung-Mo Park

Author Info. +

Department of Chemistry, Seoul National University, Seoul, Korea, Department of Chemistry, Chonbuk National University, Jeonju, Korea, Department of Biological Science, Mokpo National University, Muan, Korea, Plant Genomics and Breeding Institute, Seoul National University, Seoul, Korea, These authors contributed equally to this work.

Received: June 12, 2012; Revised: November 13, 2012; Accepted: November 14, 2012

Abstract

Go to

Dynamic dimer formation is an elaborate means of modulating transcription factor activities in diverse cellular processes.
The basic helix-loop-helix (bHLH) transcription factor LONG HYPOCOTYL IN FAR-RED 1 (HFR1), for example,
plays a role in plant photomorphogenesis by forming non-DNA binding heterodimers with PHYTOCHROMEINTERACTING
FACTORS (PIFs). Recent studies have shown that a small HLH protein KIDARI (KDR) negatively
regulates the HFR1 activity in the process. However, molecular mechanisms underlying the KDR control of the
HFR1 activity are unknown. Here, we demonstrate that KDR attenuates the HFR1 activity by competitively forming
nonfunctional heterodimers, causing liberation of PIF4 from the transcriptionally inactive HFR1-PIF4 complex.
Accordingly, the photomorphogenic hypocotyl growth of the HFR1-overexpres-sing plants can be suppressed by
KDR coexpression, as observed in the HFR1-deficient hfr1-201 mutant. These results indicate that the PIF4 activity is
modulated through a double layer of competitive inhibition by HFR1 and KDR, which could in turn ensure fine-tuning
of the PIF4 activity under fluctuating light conditions.

Keywords Arabidopsis, competitive inhibition, KIDARI (KDR), light signaling, LONG HYPOCOTYL IN FAR-RED 1 (HFR1), PHYTOCHROMEINTERACTING
FACTOR (PIF)

Article

Research Article

Mol. Cells 2013; 35(1): 25-31

Published online January 31, 2013 https://doi.org/10.1007/s10059-013-2159-2

Copyright © The Korean Society for Molecular and Cellular Biology.

A Competitive Peptide Inhibitor KIDARI Negatively Regulates HFR1 by Forming Nonfunctional Heterodimers in Arabidopsis Photomorphogenesis

Shin-Young Hong, Pil Joon Seo, Jae Yong Ryu, Shin-Hae Cho, Je-Chang Woo, and Chung-Mo Park

Department of Chemistry, Seoul National University, Seoul, Korea, Department of Chemistry, Chonbuk National University, Jeonju, Korea, Department of Biological Science, Mokpo National University, Muan, Korea, Plant Genomics and Breeding Institute, Seoul National University, Seoul, Korea, These authors contributed equally to this work.

Received: June 12, 2012; Revised: November 13, 2012; Accepted: November 14, 2012

Abstract

Dynamic dimer formation is an elaborate means of modulating transcription factor activities in diverse cellular processes.
The basic helix-loop-helix (bHLH) transcription factor LONG HYPOCOTYL IN FAR-RED 1 (HFR1), for example,
plays a role in plant photomorphogenesis by forming non-DNA binding heterodimers with PHYTOCHROMEINTERACTING
FACTORS (PIFs). Recent studies have shown that a small HLH protein KIDARI (KDR) negatively
regulates the HFR1 activity in the process. However, molecular mechanisms underlying the KDR control of the
HFR1 activity are unknown. Here, we demonstrate that KDR attenuates the HFR1 activity by competitively forming
nonfunctional heterodimers, causing liberation of PIF4 from the transcriptionally inactive HFR1-PIF4 complex.
Accordingly, the photomorphogenic hypocotyl growth of the HFR1-overexpres-sing plants can be suppressed by
KDR coexpression, as observed in the HFR1-deficient hfr1-201 mutant. These results indicate that the PIF4 activity is
modulated through a double layer of competitive inhibition by HFR1 and KDR, which could in turn ensure fine-tuning
of the PIF4 activity under fluctuating light conditions.

Keywords: Arabidopsis, competitive inhibition, KIDARI (KDR), light signaling, LONG HYPOCOTYL IN FAR-RED 1 (HFR1), PHYTOCHROMEINTERACTING
FACTOR (PIF)

Mol. Cells
Feb 28, 2023 Vol.46 No.2, pp. 69~129
COVER PICTURE
The bulk tissue is a heterogeneous mixture of various cell types, which is depicted as a skein of intertwined threads with diverse colors each of which represents a unique cell type. Single-cell omics analysis untangles efficiently the skein according to the color by providing information of molecules at individual cells and interpretation of such information based on different cell types. The molecules that can be profiled at the individual cell by single-cell omics analysis includes DNA (bottom middle), RNA (bottom right), and protein (bottom left). This special issue reviews single-cell technologies and computational methods that have been developed for the single-cell omics analysis and how they have been applied to improve our understanding of the underlying mechanisms of biological and pathological phenomena at the single-cell level.
Current Issue Archives

Share this article on

  • line
  • mail

Related articles in Mol. Cells

Molecules and Cells

eISSN 0219-1032
qr-code Download