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Mol. Cells 2012; 34(2): 177-183

Published online August 31, 2012

https://doi.org/10.1007/s10059-012-0090-6

© The Korean Society for Molecular and Cellular Biology

Dynamic Expression of Specific miRNAs during Erythroid Differentiation of Human Embryonic Stem Cells

Hong Lian Jin1,3, Jong Soo Kim1,3, Young June Kim2, Su Jin Kim1, Hal E. Broxmeyer2, and Kye-Seong Kim1,*

1Graduate School of Biomedical Science and Engineering, College of Medicine, Department of Anatomy and Cell Biology, Hanyang University, Seoul 133-791, Korea, 2Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis 46202, USA, 3These authors contributed equally to this work.

Correspondence to : *Correspondence: ks66kim@hanyang.ac.kr

Received: March 23, 2012; Revised: April 16, 2012; Accepted: May 16, 2012

Abstract

MicroRNAs (miRNAs) are small non-coding RNAs that re-gulate gene expression at post-transcriptional levels through mRNA degradation or translation inhibition. Little is known regarding miRNA participation in regulating he-matopoietic, or more specifically erythroid differentiation. This study was aimed at identifying erythroid lineage-specific miRNAs expressed during in vitro erythropoiesis using human embryonic stem cells (hESCs) and human umbilical cord blood (CB) CD34+ cells. CD34+ hematopoietic cells were produced from hESCs in vitro and subsequently induced to differentiate into erythroid cells by culture in sequence on OP9 feeder cells and then with mesenchymal stromal cells (MSC) in the presence of cytokines. Expression profiles of erythroid lineage-specific miRNAs were analyzed by quantitative PCR during in vitro differentiation. Expression levels of miR-142-3p, miR-142-5p, miR-146a and miR-451 were dynamically changed during differentiation of hESCs to CD34+ hematopoietic cells, and in subsequent differentiation of the CD34+ cells into the erythroid lineage. This suggests that these four miRNAs might be involved in regulating erythropoiesis.

Keywords dynamic expression, erythrogenesis, human embryonic stem cells, miRNAs

Article

Research Article

Mol. Cells 2012; 34(2): 177-183

Published online August 31, 2012 https://doi.org/10.1007/s10059-012-0090-6

Copyright © The Korean Society for Molecular and Cellular Biology.

Dynamic Expression of Specific miRNAs during Erythroid Differentiation of Human Embryonic Stem Cells

Hong Lian Jin1,3, Jong Soo Kim1,3, Young June Kim2, Su Jin Kim1, Hal E. Broxmeyer2, and Kye-Seong Kim1,*

1Graduate School of Biomedical Science and Engineering, College of Medicine, Department of Anatomy and Cell Biology, Hanyang University, Seoul 133-791, Korea, 2Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis 46202, USA, 3These authors contributed equally to this work.

Correspondence to:*Correspondence: ks66kim@hanyang.ac.kr

Received: March 23, 2012; Revised: April 16, 2012; Accepted: May 16, 2012

Abstract

MicroRNAs (miRNAs) are small non-coding RNAs that re-gulate gene expression at post-transcriptional levels through mRNA degradation or translation inhibition. Little is known regarding miRNA participation in regulating he-matopoietic, or more specifically erythroid differentiation. This study was aimed at identifying erythroid lineage-specific miRNAs expressed during in vitro erythropoiesis using human embryonic stem cells (hESCs) and human umbilical cord blood (CB) CD34+ cells. CD34+ hematopoietic cells were produced from hESCs in vitro and subsequently induced to differentiate into erythroid cells by culture in sequence on OP9 feeder cells and then with mesenchymal stromal cells (MSC) in the presence of cytokines. Expression profiles of erythroid lineage-specific miRNAs were analyzed by quantitative PCR during in vitro differentiation. Expression levels of miR-142-3p, miR-142-5p, miR-146a and miR-451 were dynamically changed during differentiation of hESCs to CD34+ hematopoietic cells, and in subsequent differentiation of the CD34+ cells into the erythroid lineage. This suggests that these four miRNAs might be involved in regulating erythropoiesis.

Keywords: dynamic expression, erythrogenesis, human embryonic stem cells, miRNAs

Mol. Cells
Sep 30, 2022 Vol.45 No.9, pp. 603~672
COVER PICTURE
The Target of Rapamycin Complex (TORC) is a central regulatory hub in eukaryotes, which is well conserved in diverse plant species, including tomato (Solanum lycopersicum). Inhibition of TORC genes (SlTOR, SlLST8, and SlRAPTOR) by VIGS (virus-induced gene silencing) results in early fruit ripening in tomato. The red/ orange tomatoes are early-ripened TORC-silenced fruits, while the green tomato is a control fruit. Top, left, control fruit (TRV2-myc); top, right, TRV2-SlLST8; bottom, left, TRV2-SlTOR; bottom, right, TRV2-SlRAPTOR(Choi et al., pp. 660-672).

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