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Mol. Cells 2012; 33(6): 617-626

Published online June 30, 2012

https://doi.org/10.1007/s10059-012-0080-8

© The Korean Society for Molecular and Cellular Biology

Overexpression of Arabidopsis Translationally Controlled Tumor Protein Gene AtTCTP Enhances Drought Tolerance with Rapid ABA-Induced Stomatal Closure

Yong-Min Kim, Yun-Jeong Han, Ok-Jin Hwang, Si-Seok Lee, Ah-Young Shin, Soo Young Kim, and Jeong-Il Kim*

Department of Biotechnology and Kumho Life Science Laboratory, Chonnam National University, Gwangju 500-757, Korea

Correspondence to : *Correspondence: kimji@chonnam.ac.kr

Received: March 19, 2012; Revised: April 12, 2012; Accepted: April 13, 2012

Abstract

Translationally controlled tumor protein (TCTP), also ter-med P23 in human, belongs to a family of calcium- and tubulin-binding proteins, and it is generally regarded as a growth-regulating protein. Recently, Arabidopsis TCTP (AtTCTP) has been reported to function as an important growth regulator in plants. On the other hand, plant TCTP has been suggested to be involved in abiotic stress signaling such as aluminum, salt, and water deficit by a number of microarray or proteomic analyses. In this study, the biological functions of AtTCTP were investigated by using transgenic Arabidopsis plants overexpressing AtTCTP. Interestingly, AtTCTP overexpression enhanced drought tolerance in plants. The expression analysis showed that AtTCTP was expressed in guard cells as well as in actively growing tissues. Physiological studies of the overexpression lines showed increased ABA- and calcium-induced stomatal closure ratios and faster stomatal closing responses to ABA. Furthermore, in vitro protein-protein inte-raction analysis confirmed the interaction between AtTCTP and microtubules, and microtubule cosedimentation assays revealed that the microtubule binding of AtTCTP in-creased after calcium treatment. These results demon-strate that the overexpression of AtTCTP confers drought tolerance to plants by rapid ABA-mediated stomatal closure via the interaction with microtubules in which calcium binding enhances the interaction. Collec-tively, the present results suggest that the plant TCTP has molecular properties similar to animal TCTPs, such as tubulin- and calcium-binding, and that it functions in ABA-mediated stomatal movement, in addition to regulating the growth of plants.

Keywords abscisic acid, drought tolerance, microtubule, stomatal closure, translationally controlled tumor protein

Article

Research Article

Mol. Cells 2012; 33(6): 617-626

Published online June 30, 2012 https://doi.org/10.1007/s10059-012-0080-8

Copyright © The Korean Society for Molecular and Cellular Biology.

Overexpression of Arabidopsis Translationally Controlled Tumor Protein Gene AtTCTP Enhances Drought Tolerance with Rapid ABA-Induced Stomatal Closure

Yong-Min Kim, Yun-Jeong Han, Ok-Jin Hwang, Si-Seok Lee, Ah-Young Shin, Soo Young Kim, and Jeong-Il Kim*

Department of Biotechnology and Kumho Life Science Laboratory, Chonnam National University, Gwangju 500-757, Korea

Correspondence to:*Correspondence: kimji@chonnam.ac.kr

Received: March 19, 2012; Revised: April 12, 2012; Accepted: April 13, 2012

Abstract

Translationally controlled tumor protein (TCTP), also ter-med P23 in human, belongs to a family of calcium- and tubulin-binding proteins, and it is generally regarded as a growth-regulating protein. Recently, Arabidopsis TCTP (AtTCTP) has been reported to function as an important growth regulator in plants. On the other hand, plant TCTP has been suggested to be involved in abiotic stress signaling such as aluminum, salt, and water deficit by a number of microarray or proteomic analyses. In this study, the biological functions of AtTCTP were investigated by using transgenic Arabidopsis plants overexpressing AtTCTP. Interestingly, AtTCTP overexpression enhanced drought tolerance in plants. The expression analysis showed that AtTCTP was expressed in guard cells as well as in actively growing tissues. Physiological studies of the overexpression lines showed increased ABA- and calcium-induced stomatal closure ratios and faster stomatal closing responses to ABA. Furthermore, in vitro protein-protein inte-raction analysis confirmed the interaction between AtTCTP and microtubules, and microtubule cosedimentation assays revealed that the microtubule binding of AtTCTP in-creased after calcium treatment. These results demon-strate that the overexpression of AtTCTP confers drought tolerance to plants by rapid ABA-mediated stomatal closure via the interaction with microtubules in which calcium binding enhances the interaction. Collec-tively, the present results suggest that the plant TCTP has molecular properties similar to animal TCTPs, such as tubulin- and calcium-binding, and that it functions in ABA-mediated stomatal movement, in addition to regulating the growth of plants.

Keywords: abscisic acid, drought tolerance, microtubule, stomatal closure, translationally controlled tumor protein

Mol. Cells
Sep 30, 2022 Vol.45 No.9, pp. 603~672
COVER PICTURE
The Target of Rapamycin Complex (TORC) is a central regulatory hub in eukaryotes, which is well conserved in diverse plant species, including tomato (Solanum lycopersicum). Inhibition of TORC genes (SlTOR, SlLST8, and SlRAPTOR) by VIGS (virus-induced gene silencing) results in early fruit ripening in tomato. The red/ orange tomatoes are early-ripened TORC-silenced fruits, while the green tomato is a control fruit. Top, left, control fruit (TRV2-myc); top, right, TRV2-SlLST8; bottom, left, TRV2-SlTOR; bottom, right, TRV2-SlRAPTOR(Choi et al., pp. 660-672).

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