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Mol. Cells 2012; 33(6): 583-589

Published online May 7, 2012

https://doi.org/10.1007/s10059-012-0010-9

© The Korean Society for Molecular and Cellular Biology

Bacterial Transposons Are Co-Transferred with T-DNA to Rice Chromosomes during Agrobacterium-Mediated Transformation

Sung-Ryul Kim, and Gynheung An*

Crop Biotech Institute and Department of Plant Molecular Systems Biotech, Kyung Hee University, Yongin 446-701, Korea

Correspondence to : *Correspondence: genean@khu.ac.kr

Received: January 6, 2012; Revised: April 4, 2012; Accepted: April 5, 2012

Abstract

Agrobacterium tumefaciens is widely utilized for deliver-ing a foreign gene into a plant’s genome. We found the bacterial transposon Tn5393 in transgenic rice plants. Analysis of the flanking sequences of the transferred-DNA (T-DNA) identified that a portion of the Tn5393 sequence was present immediately next to the end of the T-DNA. Because this transposon was present in A. tumefaciens strain LBA4404, but not in EHA105 and GV3101, our findings indicated that Tn5393 was transferred from LBA4404 into the rice genome during the transformation process. We also noted that another bacterial transposon, Tn5563, is present in transgenic plants. Analyses of 331 transgenic lines revealed that 26.0% carried Tn5393 and 2.1% contained Tn5563. In most of the lines, an intact transposon was integrated into the T-DNA and transferred to the rice chromosome. More than one copy of T-DNA was introduced into the plants, often at a single locus. This resulted in T-DNA repeats of normal and transposon-carrying T-DNA that generated deletions of a portion of the T-DNA, joining the T-DNA end to the bacterial transposon. Based on these data, we suggest that one should carefully select the appropriate Agrobacterium strain to avoid undesirable transformation of such sequences.

Keywords Agrobacterium, bacterial transposon, LBA4404, rice, T-DNA, Tn5393

Article

Research Article

Mol. Cells 2012; 33(6): 583-589

Published online June 30, 2012 https://doi.org/10.1007/s10059-012-0010-9

Copyright © The Korean Society for Molecular and Cellular Biology.

Bacterial Transposons Are Co-Transferred with T-DNA to Rice Chromosomes during Agrobacterium-Mediated Transformation

Sung-Ryul Kim, and Gynheung An*

Crop Biotech Institute and Department of Plant Molecular Systems Biotech, Kyung Hee University, Yongin 446-701, Korea

Correspondence to:*Correspondence: genean@khu.ac.kr

Received: January 6, 2012; Revised: April 4, 2012; Accepted: April 5, 2012

Abstract

Agrobacterium tumefaciens is widely utilized for deliver-ing a foreign gene into a plant’s genome. We found the bacterial transposon Tn5393 in transgenic rice plants. Analysis of the flanking sequences of the transferred-DNA (T-DNA) identified that a portion of the Tn5393 sequence was present immediately next to the end of the T-DNA. Because this transposon was present in A. tumefaciens strain LBA4404, but not in EHA105 and GV3101, our findings indicated that Tn5393 was transferred from LBA4404 into the rice genome during the transformation process. We also noted that another bacterial transposon, Tn5563, is present in transgenic plants. Analyses of 331 transgenic lines revealed that 26.0% carried Tn5393 and 2.1% contained Tn5563. In most of the lines, an intact transposon was integrated into the T-DNA and transferred to the rice chromosome. More than one copy of T-DNA was introduced into the plants, often at a single locus. This resulted in T-DNA repeats of normal and transposon-carrying T-DNA that generated deletions of a portion of the T-DNA, joining the T-DNA end to the bacterial transposon. Based on these data, we suggest that one should carefully select the appropriate Agrobacterium strain to avoid undesirable transformation of such sequences.

Keywords: Agrobacterium, bacterial transposon, LBA4404, rice, T-DNA, Tn5393

Mol. Cells
Mar 31, 2023 Vol.46 No.3, pp. 131~189
COVER PICTURE
The physiologically important cytoprotective signaling in normal cells (background area in turquoise) mediated by NRF2 (blue chain) is often hijacked by cancer cells (red ball) in the tumor microenvironment (yellow area). However, the differential roles of NRF2 throughout the multistage carcinogenesis remains largely unresolved (white-colored overlapping misty areas).

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