Mol. Cells 2012; 33(6): 583-589
Published online May 7, 2012
https://doi.org/10.1007/s10059-012-0010-9
© The Korean Society for Molecular and Cellular Biology
Correspondence to : *Correspondence: genean@khu.ac.kr
Agrobacterium tumefaciens is widely utilized for deliver-ing a foreign gene into a plant’s genome. We found the bacterial transposon Tn5393 in transgenic rice plants. Analysis of the flanking sequences of the transferred-DNA (T-DNA) identified that a portion of the Tn5393 sequence was present immediately next to the end of the T-DNA. Because this transposon was present in A. tumefaciens strain LBA4404, but not in EHA105 and GV3101, our findings indicated that Tn5393 was transferred from LBA4404 into the rice genome during the transformation process. We also noted that another bacterial transposon, Tn5563, is present in transgenic plants. Analyses of 331 transgenic lines revealed that 26.0% carried Tn5393 and 2.1% contained Tn5563. In most of the lines, an intact transposon was integrated into the T-DNA and transferred to the rice chromosome. More than one copy of T-DNA was introduced into the plants, often at a single locus. This resulted in T-DNA repeats of normal and transposon-carrying T-DNA that generated deletions of a portion of the T-DNA, joining the T-DNA end to the bacterial transposon. Based on these data, we suggest that one should carefully select the appropriate Agrobacterium strain to avoid undesirable transformation of such sequences.
Keywords Agrobacterium, bacterial transposon, LBA4404, rice, T-DNA, Tn5393
Mol. Cells 2012; 33(6): 583-589
Published online June 30, 2012 https://doi.org/10.1007/s10059-012-0010-9
Copyright © The Korean Society for Molecular and Cellular Biology.
Sung-Ryul Kim, and Gynheung An*
Crop Biotech Institute and Department of Plant Molecular Systems Biotech, Kyung Hee University, Yongin 446-701, Korea
Correspondence to:*Correspondence: genean@khu.ac.kr
Agrobacterium tumefaciens is widely utilized for deliver-ing a foreign gene into a plant’s genome. We found the bacterial transposon Tn5393 in transgenic rice plants. Analysis of the flanking sequences of the transferred-DNA (T-DNA) identified that a portion of the Tn5393 sequence was present immediately next to the end of the T-DNA. Because this transposon was present in A. tumefaciens strain LBA4404, but not in EHA105 and GV3101, our findings indicated that Tn5393 was transferred from LBA4404 into the rice genome during the transformation process. We also noted that another bacterial transposon, Tn5563, is present in transgenic plants. Analyses of 331 transgenic lines revealed that 26.0% carried Tn5393 and 2.1% contained Tn5563. In most of the lines, an intact transposon was integrated into the T-DNA and transferred to the rice chromosome. More than one copy of T-DNA was introduced into the plants, often at a single locus. This resulted in T-DNA repeats of normal and transposon-carrying T-DNA that generated deletions of a portion of the T-DNA, joining the T-DNA end to the bacterial transposon. Based on these data, we suggest that one should carefully select the appropriate Agrobacterium strain to avoid undesirable transformation of such sequences.
Keywords: Agrobacterium, bacterial transposon, LBA4404, rice, T-DNA, Tn5393
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