TOP

Research Article

Split Viewer

Mol. Cells 2012; 33(5): 479-486

Published online April 17, 2012

https://doi.org/10.1007/s10059-012-2266-5

© The Korean Society for Molecular and Cellular Biology

Lactobacillus plantarum Lipoteichoic Acid Alleviates TNF-α-Induced Inflammation in the HT-29 Intestinal Epithelial Cell Line

Hangeun Kim1, Bong Jun Jung2, Ji Hae Jung2, Joo Yun Kim2, Sung Kyun Chung3, and Dae Kyun Chung2,4,5,*

1Department of Internal Medicine, Saint Louis University, St. Louis, MO 63104, USA, 2School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin 449-701, Korea, 3Department of Dental Hygiene, Shinheung College, Uijeongbu 480-701, Korea, 4Skin Biotechnology Center, Kyung Hee University, Yongin 449-701, Korea, 5RNA Inc., College of Life Science, Kyung Hee University, Yongin 449-701, Korea

Correspondence to : *Correspondence: dkchung@khu.ac.kr

Received: November 22, 2011; Revised: March 8, 2012; Accepted: March 8, 2012

Abstract

We recently observed that lipoteichoic acid (LTA) isolated from Lactobacillus plantarum inhibited endotoxin-me-diated inflammation of the immune cells and septic shock in a mouse model. Here, we examined the inhibitory role of L. plantarum LTA (pLTA) on the inflammatory responses of intestinal epithelial cells (IEC). The human colon cell line, HT-29, increased interleukin (IL)-8 expression in response to recombinant human tumor necrosis factor (TNF)-alpha, but not in response to bacterial ligands and interferon (IFN)-gamma. TNF-alpha also increased the production of inducible nitric oxide synthase (iNOS), nitric oxide (NO), and intercellular adhesion molecule 1 (ICAM-1) through activation of p38 mitogen-activated protein kinase (MAPK) from HT-29 cells. However, the inflammatory response of HT-29 on TNF-alpha stimulation was significantly inhibited by pLTA treatment. This pLTA-mediated inhibition accompanied the inhibition of nuclear factor (NF)-kappa B and MAPKs. Our data suggest that pLTA regulates cytokine-mediated immune responses and may be a good candidate for maintaining intestinal homeostasis against excessive inflammation.

Keywords colon, cytokine, HT-29, inflammation, lipoteichoic acid

Article

Research Article

Mol. Cells 2012; 33(5): 479-486

Published online May 31, 2012 https://doi.org/10.1007/s10059-012-2266-5

Copyright © The Korean Society for Molecular and Cellular Biology.

Lactobacillus plantarum Lipoteichoic Acid Alleviates TNF-α-Induced Inflammation in the HT-29 Intestinal Epithelial Cell Line

Hangeun Kim1, Bong Jun Jung2, Ji Hae Jung2, Joo Yun Kim2, Sung Kyun Chung3, and Dae Kyun Chung2,4,5,*

1Department of Internal Medicine, Saint Louis University, St. Louis, MO 63104, USA, 2School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin 449-701, Korea, 3Department of Dental Hygiene, Shinheung College, Uijeongbu 480-701, Korea, 4Skin Biotechnology Center, Kyung Hee University, Yongin 449-701, Korea, 5RNA Inc., College of Life Science, Kyung Hee University, Yongin 449-701, Korea

Correspondence to:*Correspondence: dkchung@khu.ac.kr

Received: November 22, 2011; Revised: March 8, 2012; Accepted: March 8, 2012

Abstract

We recently observed that lipoteichoic acid (LTA) isolated from Lactobacillus plantarum inhibited endotoxin-me-diated inflammation of the immune cells and septic shock in a mouse model. Here, we examined the inhibitory role of L. plantarum LTA (pLTA) on the inflammatory responses of intestinal epithelial cells (IEC). The human colon cell line, HT-29, increased interleukin (IL)-8 expression in response to recombinant human tumor necrosis factor (TNF)-alpha, but not in response to bacterial ligands and interferon (IFN)-gamma. TNF-alpha also increased the production of inducible nitric oxide synthase (iNOS), nitric oxide (NO), and intercellular adhesion molecule 1 (ICAM-1) through activation of p38 mitogen-activated protein kinase (MAPK) from HT-29 cells. However, the inflammatory response of HT-29 on TNF-alpha stimulation was significantly inhibited by pLTA treatment. This pLTA-mediated inhibition accompanied the inhibition of nuclear factor (NF)-kappa B and MAPKs. Our data suggest that pLTA regulates cytokine-mediated immune responses and may be a good candidate for maintaining intestinal homeostasis against excessive inflammation.

Keywords: colon, cytokine, HT-29, inflammation, lipoteichoic acid

Mol. Cells
Feb 28, 2023 Vol.46 No.2, pp. 69~129
COVER PICTURE
The bulk tissue is a heterogeneous mixture of various cell types, which is depicted as a skein of intertwined threads with diverse colors each of which represents a unique cell type. Single-cell omics analysis untangles efficiently the skein according to the color by providing information of molecules at individual cells and interpretation of such information based on different cell types. The molecules that can be profiled at the individual cell by single-cell omics analysis includes DNA (bottom middle), RNA (bottom right), and protein (bottom left). This special issue reviews single-cell technologies and computational methods that have been developed for the single-cell omics analysis and how they have been applied to improve our understanding of the underlying mechanisms of biological and pathological phenomena at the single-cell level.

Share this article on

  • line
  • mail

Related articles in Mol. Cells

Molecules and Cells

eISSN 0219-1032
qr-code Download