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Mol. Cells 2012; 33(5): 479-486

Published online May 31, 2012

https://doi.org/10.1007/s10059-012-2266-5

© The Korean Society for Molecular and Cellular Biology

Lactobacillus plantarum Lipoteichoic Acid Alleviates TNF-α-Induced Inflammation in the HT-29 Intestinal Epithelial Cell Line

Hangeun Kim1, Bong Jun Jung2, Ji Hae Jung2, Joo Yun Kim2, Sung Kyun Chung3, and Dae Kyun Chung2,4,5,*

1Department of Internal Medicine, Saint Louis University, St. Louis, MO 63104, USA, 2School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin 449-701, Korea, 3Department of Dental Hygiene, Shinheung College, Uijeongbu 480-701, Korea, 4Skin Biotechnology Center, Kyung Hee University, Yongin 449-701, Korea, 5RNA Inc., College of Life Science, Kyung Hee University, Yongin 449-701, Korea

Correspondence to : *Correspondence: dkchung@khu.ac.kr

Received: November 22, 2011; Revised: March 8, 2012; Accepted: March 8, 2012

Abstract

We recently observed that lipoteichoic acid (LTA) isolated from Lactobacillus plantarum inhibited endotoxin-me-diated inflammation of the immune cells and septic shock in a mouse model. Here, we examined the inhibitory role of L. plantarum LTA (pLTA) on the inflammatory responses of intestinal epithelial cells (IEC). The human colon cell line, HT-29, increased interleukin (IL)-8 expression in response to recombinant human tumor necrosis factor (TNF)-alpha, but not in response to bacterial ligands and interferon (IFN)-gamma. TNF-alpha also increased the production of inducible nitric oxide synthase (iNOS), nitric oxide (NO), and intercellular adhesion molecule 1 (ICAM-1) through activation of p38 mitogen-activated protein kinase (MAPK) from HT-29 cells. However, the inflammatory response of HT-29 on TNF-alpha stimulation was significantly inhibited by pLTA treatment. This pLTA-mediated inhibition accompanied the inhibition of nuclear factor (NF)-kappa B and MAPKs. Our data suggest that pLTA regulates cytokine-mediated immune responses and may be a good candidate for maintaining intestinal homeostasis against excessive inflammation.

Keywords colon, cytokine, HT-29, inflammation, lipoteichoic acid

Article

Research Article

Mol. Cells 2012; 33(5): 479-486

Published online May 31, 2012 https://doi.org/10.1007/s10059-012-2266-5

Copyright © The Korean Society for Molecular and Cellular Biology.

Lactobacillus plantarum Lipoteichoic Acid Alleviates TNF-α-Induced Inflammation in the HT-29 Intestinal Epithelial Cell Line

Hangeun Kim1, Bong Jun Jung2, Ji Hae Jung2, Joo Yun Kim2, Sung Kyun Chung3, and Dae Kyun Chung2,4,5,*

1Department of Internal Medicine, Saint Louis University, St. Louis, MO 63104, USA, 2School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin 449-701, Korea, 3Department of Dental Hygiene, Shinheung College, Uijeongbu 480-701, Korea, 4Skin Biotechnology Center, Kyung Hee University, Yongin 449-701, Korea, 5RNA Inc., College of Life Science, Kyung Hee University, Yongin 449-701, Korea

Correspondence to:*Correspondence: dkchung@khu.ac.kr

Received: November 22, 2011; Revised: March 8, 2012; Accepted: March 8, 2012

Abstract

We recently observed that lipoteichoic acid (LTA) isolated from Lactobacillus plantarum inhibited endotoxin-me-diated inflammation of the immune cells and septic shock in a mouse model. Here, we examined the inhibitory role of L. plantarum LTA (pLTA) on the inflammatory responses of intestinal epithelial cells (IEC). The human colon cell line, HT-29, increased interleukin (IL)-8 expression in response to recombinant human tumor necrosis factor (TNF)-alpha, but not in response to bacterial ligands and interferon (IFN)-gamma. TNF-alpha also increased the production of inducible nitric oxide synthase (iNOS), nitric oxide (NO), and intercellular adhesion molecule 1 (ICAM-1) through activation of p38 mitogen-activated protein kinase (MAPK) from HT-29 cells. However, the inflammatory response of HT-29 on TNF-alpha stimulation was significantly inhibited by pLTA treatment. This pLTA-mediated inhibition accompanied the inhibition of nuclear factor (NF)-kappa B and MAPKs. Our data suggest that pLTA regulates cytokine-mediated immune responses and may be a good candidate for maintaining intestinal homeostasis against excessive inflammation.

Keywords: colon, cytokine, HT-29, inflammation, lipoteichoic acid

Mol. Cells
Sep 30, 2022 Vol.45 No.9, pp. 603~672
COVER PICTURE
The Target of Rapamycin Complex (TORC) is a central regulatory hub in eukaryotes, which is well conserved in diverse plant species, including tomato (Solanum lycopersicum). Inhibition of TORC genes (SlTOR, SlLST8, and SlRAPTOR) by VIGS (virus-induced gene silencing) results in early fruit ripening in tomato. The red/ orange tomatoes are early-ripened TORC-silenced fruits, while the green tomato is a control fruit. Top, left, control fruit (TRV2-myc); top, right, TRV2-SlLST8; bottom, left, TRV2-SlTOR; bottom, right, TRV2-SlRAPTOR(Choi et al., pp. 660-672).

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