TOP

Research Article

Split Viewer

Mol. Cells 2012; 33(4): 407-414

Published online March 5, 2012

https://doi.org/10.1007/s10059-012-0011-8

© The Korean Society for Molecular and Cellular Biology

LIME Mediates Immunological Synapse Formation through Activation of VAV

Myoungsun Son, Inyoung Park, Ok-Hee Lee, Inmoo Rhee, Changwon Park, and Yungdae Yun*

Department of Life Science, Ewha Womans’ University, Seoul 120-750, Korea

Correspondence to : *Correspondence: YUNYUNG@ewha.ac.kr

Received: January 9, 2012; Accepted: January 30, 2012

Abstract

Lck Interacting Membrane protein (LIME) was previously characterized as a transmembrane adaptor protein media-ting TCR-dependent T cell activation. Here, we show that LIME associates with Vav in response to TCR stimulation and is required for Vav guanine nucleotide exchange factor (GEF) activity for Rac1. Consistent with this finding, actin polymerization at the immunological synapse (IS) was markedly enhanced by overexpression of LIME, but was reduced by expression of a LIME shRNA. Moreover, TCR-mediated cell adhesion to ICAM-1, laminin, or fibro-nectin was downregulated by expression of LIME shRNA. In addition, in the IS, LIME but not LAT was found to localize at the peripheral-supramolecular activation cluster (p-SMAC) where the integrins were previously shown to be localized. Together, these results establish LIME as a transmembrane adaptor protein linking TCR stimulation to IS formation and integrin activation through activation of Vav.

Keywords adhesion, immunological synapse, integrin, LIME, VAV

Article

Research Article

Mol. Cells 2012; 33(4): 407-414

Published online April 30, 2012 https://doi.org/10.1007/s10059-012-0011-8

Copyright © The Korean Society for Molecular and Cellular Biology.

LIME Mediates Immunological Synapse Formation through Activation of VAV

Myoungsun Son, Inyoung Park, Ok-Hee Lee, Inmoo Rhee, Changwon Park, and Yungdae Yun*

Department of Life Science, Ewha Womans’ University, Seoul 120-750, Korea

Correspondence to:*Correspondence: YUNYUNG@ewha.ac.kr

Received: January 9, 2012; Accepted: January 30, 2012

Abstract

Lck Interacting Membrane protein (LIME) was previously characterized as a transmembrane adaptor protein media-ting TCR-dependent T cell activation. Here, we show that LIME associates with Vav in response to TCR stimulation and is required for Vav guanine nucleotide exchange factor (GEF) activity for Rac1. Consistent with this finding, actin polymerization at the immunological synapse (IS) was markedly enhanced by overexpression of LIME, but was reduced by expression of a LIME shRNA. Moreover, TCR-mediated cell adhesion to ICAM-1, laminin, or fibro-nectin was downregulated by expression of LIME shRNA. In addition, in the IS, LIME but not LAT was found to localize at the peripheral-supramolecular activation cluster (p-SMAC) where the integrins were previously shown to be localized. Together, these results establish LIME as a transmembrane adaptor protein linking TCR stimulation to IS formation and integrin activation through activation of Vav.

Keywords: adhesion, immunological synapse, integrin, LIME, VAV

Mol. Cells
May 31, 2023 Vol.46 No.5, pp. 259~328
COVER PICTURE
The alpha-helices in the lamin filaments are depicted as coils, with different subdomains distinguished by various colors. Coil 1a is represented by magenta, coil 1b by yellow, L2 by green, coil 2a by white, coil 2b by brown, stutter by cyan, coil 2c by dark blue, and the lamin Ig-like domain by grey. In the background, cells are displayed, with the cytosol depicted in green and the nucleus in blue (Ahn et al., pp. 309-318).

Share this article on

  • line
  • mail

Related articles in Mol. Cells

Molecules and Cells

eISSN 0219-1032
qr-code Download