Mol. Cells 2012; 33(4): 407-414
Published online March 5, 2012
https://doi.org/10.1007/s10059-012-0011-8
© The Korean Society for Molecular and Cellular Biology
Correspondence to : *Correspondence: YUNYUNG@ewha.ac.kr
Lck Interacting Membrane protein (LIME) was previously characterized as a transmembrane adaptor protein media-ting TCR-dependent T cell activation. Here, we show that LIME associates with Vav in response to TCR stimulation and is required for Vav guanine nucleotide exchange factor (GEF) activity for Rac1. Consistent with this finding, actin polymerization at the immunological synapse (IS) was markedly enhanced by overexpression of LIME, but was reduced by expression of a LIME shRNA. Moreover, TCR-mediated cell adhesion to ICAM-1, laminin, or fibro-nectin was downregulated by expression of LIME shRNA. In addition, in the IS, LIME but not LAT was found to localize at the peripheral-supramolecular activation cluster (p-SMAC) where the integrins were previously shown to be localized. Together, these results establish LIME as a transmembrane adaptor protein linking TCR stimulation to IS formation and integrin activation through activation of Vav.
Keywords adhesion, immunological synapse, integrin, LIME, VAV
Mol. Cells 2012; 33(4): 407-414
Published online April 30, 2012 https://doi.org/10.1007/s10059-012-0011-8
Copyright © The Korean Society for Molecular and Cellular Biology.
Myoungsun Son, Inyoung Park, Ok-Hee Lee, Inmoo Rhee, Changwon Park, and Yungdae Yun*
Department of Life Science, Ewha Womans’ University, Seoul 120-750, Korea
Correspondence to:*Correspondence: YUNYUNG@ewha.ac.kr
Lck Interacting Membrane protein (LIME) was previously characterized as a transmembrane adaptor protein media-ting TCR-dependent T cell activation. Here, we show that LIME associates with Vav in response to TCR stimulation and is required for Vav guanine nucleotide exchange factor (GEF) activity for Rac1. Consistent with this finding, actin polymerization at the immunological synapse (IS) was markedly enhanced by overexpression of LIME, but was reduced by expression of a LIME shRNA. Moreover, TCR-mediated cell adhesion to ICAM-1, laminin, or fibro-nectin was downregulated by expression of LIME shRNA. In addition, in the IS, LIME but not LAT was found to localize at the peripheral-supramolecular activation cluster (p-SMAC) where the integrins were previously shown to be localized. Together, these results establish LIME as a transmembrane adaptor protein linking TCR stimulation to IS formation and integrin activation through activation of Vav.
Keywords: adhesion, immunological synapse, integrin, LIME, VAV
Chaoyuan Sun, Junfang Zheng, Shan Cheng, Duiping Feng, and Junqi He
Mol. Cells 2013; 36(1): 47-54 https://doi.org/10.1007/s10059-013-0014-0Jeongsuk Choi, Jeasun Choi, and Sang-Uk Nham*
Mol. Cells 2010; 30(3): 227-234 https://doi.org/10.1007/s10059-010-0111-2Hyo-Jong Lee, Bum Ju Ahn, Min Wook Shin, Jeong-Hyun Choi, and Kyu-Won Kim*
Mol. Cells 2010; 29(3): 223-227 https://doi.org/10.1007/s10059-010-0043-x