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Mol. Cells 2012; 33(4): 407-414

Published online March 5, 2012

https://doi.org/10.1007/s10059-012-0011-8

© The Korean Society for Molecular and Cellular Biology

LIME Mediates Immunological Synapse Formation through Activation of VAV

Myoungsun Son, Inyoung Park, Ok-Hee Lee, Inmoo Rhee, Changwon Park, and Yungdae Yun*

Department of Life Science, Ewha Womans’ University, Seoul 120-750, Korea

Correspondence to : *Correspondence: YUNYUNG@ewha.ac.kr

Received: January 9, 2012; Accepted: January 30, 2012

Abstract

Lck Interacting Membrane protein (LIME) was previously characterized as a transmembrane adaptor protein media-ting TCR-dependent T cell activation. Here, we show that LIME associates with Vav in response to TCR stimulation and is required for Vav guanine nucleotide exchange factor (GEF) activity for Rac1. Consistent with this finding, actin polymerization at the immunological synapse (IS) was markedly enhanced by overexpression of LIME, but was reduced by expression of a LIME shRNA. Moreover, TCR-mediated cell adhesion to ICAM-1, laminin, or fibro-nectin was downregulated by expression of LIME shRNA. In addition, in the IS, LIME but not LAT was found to localize at the peripheral-supramolecular activation cluster (p-SMAC) where the integrins were previously shown to be localized. Together, these results establish LIME as a transmembrane adaptor protein linking TCR stimulation to IS formation and integrin activation through activation of Vav.

Keywords adhesion, immunological synapse, integrin, LIME, VAV

Article

Research Article

Mol. Cells 2012; 33(4): 407-414

Published online April 30, 2012 https://doi.org/10.1007/s10059-012-0011-8

Copyright © The Korean Society for Molecular and Cellular Biology.

LIME Mediates Immunological Synapse Formation through Activation of VAV

Myoungsun Son, Inyoung Park, Ok-Hee Lee, Inmoo Rhee, Changwon Park, and Yungdae Yun*

Department of Life Science, Ewha Womans’ University, Seoul 120-750, Korea

Correspondence to:*Correspondence: YUNYUNG@ewha.ac.kr

Received: January 9, 2012; Accepted: January 30, 2012

Abstract

Lck Interacting Membrane protein (LIME) was previously characterized as a transmembrane adaptor protein media-ting TCR-dependent T cell activation. Here, we show that LIME associates with Vav in response to TCR stimulation and is required for Vav guanine nucleotide exchange factor (GEF) activity for Rac1. Consistent with this finding, actin polymerization at the immunological synapse (IS) was markedly enhanced by overexpression of LIME, but was reduced by expression of a LIME shRNA. Moreover, TCR-mediated cell adhesion to ICAM-1, laminin, or fibro-nectin was downregulated by expression of LIME shRNA. In addition, in the IS, LIME but not LAT was found to localize at the peripheral-supramolecular activation cluster (p-SMAC) where the integrins were previously shown to be localized. Together, these results establish LIME as a transmembrane adaptor protein linking TCR stimulation to IS formation and integrin activation through activation of Vav.

Keywords: adhesion, immunological synapse, integrin, LIME, VAV

Mol. Cells
Nov 30, 2023 Vol.46 No.11, pp. 655~725
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Kim et al. (pp. 710-724) demonstrated that a pathogen-derived Ralstonia pseudosolanacearum type III effector RipL delays flowering time and enhances susceptibility to bacterial infection in Arabidopsis thaliana. Shown is the RipL-expressing Arabidopsis plant, which displays general dampening of the transcriptional program during pathogen infection, grown in long-day conditions.

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