TOP

Research Article

Split Viewer

Mol. Cells 2012; 33(3): 269-275

Published online January 3, 2012

https://doi.org/10.1007/s10059-012-2231-3

© The Korean Society for Molecular and Cellular Biology

Activation of Rice nicotianamine synthase 2 (OsNAS2) Enhances Iron Availability for Biofortification

Sichul Lee1, You-Sun Kim2, Un Sil Jeon3, Yoon-Keun Kim2, Jan K. Schjoerring4, and Gynheung An5,*

1Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire, 03755, USA, 2Department of Life Science, Pohang University of Science and Technology, Pohang 790-784, Korea, 3Department of Internal Medicine, Korea University Guro Hospital, Seoul 152-703, Korea, 4Plant and Soil Science Laboratory, Department of Agriculture and Ecology, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark, 5Department of Plant Molecular Systems Biotechnology and Crop Biotech Center, Kyung Hee University, Yongin 446-701, Korea

Correspondence to : *Correspondence: genean@khu.ac.kr

Received: October 19, 2011; Revised: November 24, 2011; Accepted: November 25, 2011

Abstract

Because micronutrients in human diets ultimately come from plant sources, malnutrition of essential minerals is a significant public health concern. By increasing the expression of nicotianamine synthase (NAS), we fortified the level of bioavailable iron in rice seeds. Activation of iron deficiency-inducible OsNAS2 resulted in a rise in Fe content (3.0-fold) in mature seeds. Its ectopic expression also increased that content. Enhanced expression led to higher tolerance of Fe deficiency and better growth under elevated pH. Mice fed with OsNAS2-D1 seeds recovered more rapidly from anemia, indicating that bioavailable Fe contents were improved by this increase in OsNAS2 expression.

Keywords anemia, bioavailability, mouse, rice

Article

Research Article

Mol. Cells 2012; 33(3): 269-275

Published online March 31, 2012 https://doi.org/10.1007/s10059-012-2231-3

Copyright © The Korean Society for Molecular and Cellular Biology.

Activation of Rice nicotianamine synthase 2 (OsNAS2) Enhances Iron Availability for Biofortification

Sichul Lee1, You-Sun Kim2, Un Sil Jeon3, Yoon-Keun Kim2, Jan K. Schjoerring4, and Gynheung An5,*

1Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire, 03755, USA, 2Department of Life Science, Pohang University of Science and Technology, Pohang 790-784, Korea, 3Department of Internal Medicine, Korea University Guro Hospital, Seoul 152-703, Korea, 4Plant and Soil Science Laboratory, Department of Agriculture and Ecology, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark, 5Department of Plant Molecular Systems Biotechnology and Crop Biotech Center, Kyung Hee University, Yongin 446-701, Korea

Correspondence to:*Correspondence: genean@khu.ac.kr

Received: October 19, 2011; Revised: November 24, 2011; Accepted: November 25, 2011

Abstract

Because micronutrients in human diets ultimately come from plant sources, malnutrition of essential minerals is a significant public health concern. By increasing the expression of nicotianamine synthase (NAS), we fortified the level of bioavailable iron in rice seeds. Activation of iron deficiency-inducible OsNAS2 resulted in a rise in Fe content (3.0-fold) in mature seeds. Its ectopic expression also increased that content. Enhanced expression led to higher tolerance of Fe deficiency and better growth under elevated pH. Mice fed with OsNAS2-D1 seeds recovered more rapidly from anemia, indicating that bioavailable Fe contents were improved by this increase in OsNAS2 expression.

Keywords: anemia, bioavailability, mouse, rice

Mol. Cells
Mar 31, 2023 Vol.46 No.3, pp. 131~189
COVER PICTURE
The physiologically important cytoprotective signaling in normal cells (background area in turquoise) mediated by NRF2 (blue chain) is often hijacked by cancer cells (red ball) in the tumor microenvironment (yellow area). However, the differential roles of NRF2 throughout the multistage carcinogenesis remains largely unresolved (white-colored overlapping misty areas).

Supplementary File

Share this article on

  • line
  • mail

Related articles in Mol. Cells

Molecules and Cells

eISSN 0219-1032
qr-code Download