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Mol. Cells 2012; 33(2): 203-210

Published online February 29, 2012

https://doi.org/10.1007/s10059-012-2271-8

© The Korean Society for Molecular and Cellular Biology

Moesin Is a Biomarker for the Assessment of Genotoxic Carcinogens in Mouse Lymphoma

Yoen Jung Lee1,2, In-Kwon Choi1,2, Yhun Yhong Sheen3, Sue Nie Park4, and Ho Jeong Kwon1,2,*

1Department of Biotechnology, Yonsei University, Seoul 120-749, Korea, 2Translational Research Center for Protein Function Control, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Korea, 3College of Pharmacy, Ewha Womans University, Seoul 120-750, Korea, 4Hazardous Substances Analysis Division at Seoul Regional FDA, Korea Food and Drug Administration, Seoul 158-050, Korea

Correspondence to : *Correspondence: kwonhj@yonsei.ac.kr

Received: November 29, 2011; Accepted: December 7, 2011

Abstract

1,2-Dibromoethane and glycidol are well known genotoxic carcinogens, which have been widely used in industry. To identify a specific biomarker for these car-cinogens in cells, the cellular proteome of L5178Y mouse lymphoma cells treated with these compounds was ana-lyzed by 2-dimen-sional gel electrophoresis (2-DE) and MALDI-TOF mass spectrometry (MS). Of 50 protein spots showing a greater than 1.5-fold increase or decrease in intensity compared to control cells on a 2-D gel, we fo-cused on the candidate biomarker moesin. Western analysis using monoclonal rabbit anti-moesin confirmed the identity of the protein and its increased level of expression upon exposure to the carcinogenic compounds. Moesin expression also increased in cells treated with six additional genotoxic carcinogens, verifying that moesin could serve as a biomarker to monitor phenotypic change upon exposure to genotoxic carcinogens in L5178Y mouse lymphoma cells.

Keywords biomarker, genotoxic carcinogen, moesin, toxicoproteomics

Article

Research Article

Mol. Cells 2012; 33(2): 203-210

Published online February 29, 2012 https://doi.org/10.1007/s10059-012-2271-8

Copyright © The Korean Society for Molecular and Cellular Biology.

Moesin Is a Biomarker for the Assessment of Genotoxic Carcinogens in Mouse Lymphoma

Yoen Jung Lee1,2, In-Kwon Choi1,2, Yhun Yhong Sheen3, Sue Nie Park4, and Ho Jeong Kwon1,2,*

1Department of Biotechnology, Yonsei University, Seoul 120-749, Korea, 2Translational Research Center for Protein Function Control, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Korea, 3College of Pharmacy, Ewha Womans University, Seoul 120-750, Korea, 4Hazardous Substances Analysis Division at Seoul Regional FDA, Korea Food and Drug Administration, Seoul 158-050, Korea

Correspondence to:*Correspondence: kwonhj@yonsei.ac.kr

Received: November 29, 2011; Accepted: December 7, 2011

Abstract

1,2-Dibromoethane and glycidol are well known genotoxic carcinogens, which have been widely used in industry. To identify a specific biomarker for these car-cinogens in cells, the cellular proteome of L5178Y mouse lymphoma cells treated with these compounds was ana-lyzed by 2-dimen-sional gel electrophoresis (2-DE) and MALDI-TOF mass spectrometry (MS). Of 50 protein spots showing a greater than 1.5-fold increase or decrease in intensity compared to control cells on a 2-D gel, we fo-cused on the candidate biomarker moesin. Western analysis using monoclonal rabbit anti-moesin confirmed the identity of the protein and its increased level of expression upon exposure to the carcinogenic compounds. Moesin expression also increased in cells treated with six additional genotoxic carcinogens, verifying that moesin could serve as a biomarker to monitor phenotypic change upon exposure to genotoxic carcinogens in L5178Y mouse lymphoma cells.

Keywords: biomarker, genotoxic carcinogen, moesin, toxicoproteomics

Mol. Cells
Nov 30, 2022 Vol.45 No.11, pp. 763~867
COVER PICTURE
Naive (cyan) and axotomized (magenta) retinal ganglion cell axons in Xenopus tropicalis (Choi et al., pp. 846-854).

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