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Mol. Cells 2012; 33(1): 71-77

Published online January 31, 2012

https://doi.org/10.1007/s10059-012-2214-4

© The Korean Society for Molecular and Cellular Biology

Selection and Characterization of Tenascin C Targeting Peptide

Mee Young Kim1,8,*, Ok Ran Kim1,2,8, Yong Seok Choi1, Heuiran Lee3, Keerang Park4, Choon-Taek Lee5,6, Keon Wook Kang7, and Sunjoo Jeong1,*

1National Research Laboratory for RNA Cell Biology, Brain Korea 21 Graduate Program for RNA Biology, Institute of Nanosensor and Biotechnology, and Department of Molecular Biology, Dankook University, Yongin 448-701, Korea, 2Division of Cardiology, Catholic University College of Medicine, Seoul 137-701, Korea, 3Department of Microbiology, University of Ulsan College of Medicine, Seoul 138-736, Korea, 4JS Gene Therapy R&D Center, Jooseong College, Cheongwon 363-794, Korea, 5Department of Internal Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea, 6Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam 463-707, Korea, 7Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea, 8These authors contributed equally to this work.

Correspondence to : *Correspondence: sjsj@dankook.ac.kr (SJ); iris1774@dankook.ac.kr (MYK)

Received: October 4, 2011; Revised: November 7, 2011; Accepted: November 8, 2011

Abstract

Since tenascin C is a factor expressed highly in the tu-mor-associated matrix, it would be a desirable first step for targeting the tumor-specific microenvironment. In fact, a high level of tenascin C expression has been re-ported in most solid tumors, including lung cancer, colon cancer and glioblastoma. Therefore, the targeted binding of tenascin C in tumor stroma would inhibit tumor metastasis by modulating cancer cell growth and migration. We isolated a peptide that bound to tenascin C by phage display peptide library selection, and the selected peptide specifically recognized tenascin C protein in xenograft mouse tissue. We also observed exclusive staining of tenascin C by the selected peptide in tumor patient tissues. Moreover, the peptide reduced tenascin C-induced cell rounding and migration. We propose that the tenascin C targeting peptide may be useful as a specific anti-cancer diagnostic and therapeutic tool for most human solid tumors.

Keywords anti-cancer therapeutic, peptide, tenascin C

Article

Research Article

Mol. Cells 2012; 33(1): 71-77

Published online January 31, 2012 https://doi.org/10.1007/s10059-012-2214-4

Copyright © The Korean Society for Molecular and Cellular Biology.

Selection and Characterization of Tenascin C Targeting Peptide

Mee Young Kim1,8,*, Ok Ran Kim1,2,8, Yong Seok Choi1, Heuiran Lee3, Keerang Park4, Choon-Taek Lee5,6, Keon Wook Kang7, and Sunjoo Jeong1,*

1National Research Laboratory for RNA Cell Biology, Brain Korea 21 Graduate Program for RNA Biology, Institute of Nanosensor and Biotechnology, and Department of Molecular Biology, Dankook University, Yongin 448-701, Korea, 2Division of Cardiology, Catholic University College of Medicine, Seoul 137-701, Korea, 3Department of Microbiology, University of Ulsan College of Medicine, Seoul 138-736, Korea, 4JS Gene Therapy R&D Center, Jooseong College, Cheongwon 363-794, Korea, 5Department of Internal Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea, 6Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam 463-707, Korea, 7Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea, 8These authors contributed equally to this work.

Correspondence to:*Correspondence: sjsj@dankook.ac.kr (SJ); iris1774@dankook.ac.kr (MYK)

Received: October 4, 2011; Revised: November 7, 2011; Accepted: November 8, 2011

Abstract

Since tenascin C is a factor expressed highly in the tu-mor-associated matrix, it would be a desirable first step for targeting the tumor-specific microenvironment. In fact, a high level of tenascin C expression has been re-ported in most solid tumors, including lung cancer, colon cancer and glioblastoma. Therefore, the targeted binding of tenascin C in tumor stroma would inhibit tumor metastasis by modulating cancer cell growth and migration. We isolated a peptide that bound to tenascin C by phage display peptide library selection, and the selected peptide specifically recognized tenascin C protein in xenograft mouse tissue. We also observed exclusive staining of tenascin C by the selected peptide in tumor patient tissues. Moreover, the peptide reduced tenascin C-induced cell rounding and migration. We propose that the tenascin C targeting peptide may be useful as a specific anti-cancer diagnostic and therapeutic tool for most human solid tumors.

Keywords: anti-cancer therapeutic, peptide, tenascin C

Mol. Cells
Aug 31, 2022 Vol.45 No.8, pp. 513~602
COVER PICTURE
Cryo-EM structure of human porphyrin transporter ABCB6 (main figure) shows that binding of hemin (inset, magenta) in concert with two glutathione molecules (cyan) primes ABCB6 for high ATP turnover (Kim et al., pp. 575-587).

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