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Mol. Cells 2012; 33(1): 71-77
Published online January 31, 2012
https://doi.org/10.1007/s10059-012-2214-4
© The Korean Society for Molecular and Cellular Biology
Selection and Characterization of Tenascin C Targeting Peptide
Correspondence to : *Correspondence: sjsj@dankook.ac.kr (SJ); iris1774@dankook.ac.kr (MYK)
Since tenascin C is a factor expressed highly in the tu-mor-associated matrix, it would be a desirable first step for targeting the tumor-specific microenvironment. In fact, a high level of tenascin C expression has been re-ported in most solid tumors, including lung cancer, colon cancer and glioblastoma. Therefore, the targeted binding of tenascin C in tumor stroma would inhibit tumor metastasis by modulating cancer cell growth and migration. We isolated a peptide that bound to tenascin C by phage display peptide library selection, and the selected peptide specifically recognized tenascin C protein in xenograft mouse tissue. We also observed exclusive staining of tenascin C by the selected peptide in tumor patient tissues. Moreover, the peptide reduced tenascin C-induced cell rounding and migration. We propose that the tenascin C targeting peptide may be useful as a specific anti-cancer diagnostic and therapeutic tool for most human solid tumors.
Keywords anti-cancer therapeutic, peptide, tenascin C
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Research Article
Mol. Cells 2012; 33(1): 71-77
Published online January 31, 2012 https://doi.org/10.1007/s10059-012-2214-4
Copyright © The Korean Society for Molecular and Cellular Biology.
Selection and Characterization of Tenascin C Targeting Peptide
Mee Young Kim1,8,*, Ok Ran Kim1,2,8, Yong Seok Choi1, Heuiran Lee3, Keerang Park4, Choon-Taek Lee5,6, Keon Wook Kang7, and Sunjoo Jeong1,*
1National Research Laboratory for RNA Cell Biology, Brain Korea 21 Graduate Program for RNA Biology, Institute of Nanosensor and Biotechnology, and Department of Molecular Biology, Dankook University, Yongin 448-701, Korea, 2Division of Cardiology, Catholic University College of Medicine, Seoul 137-701, Korea, 3Department of Microbiology, University of Ulsan College of Medicine, Seoul 138-736, Korea, 4JS Gene Therapy R＆D Center, Jooseong College, Cheongwon 363-794, Korea, 5Department of Internal Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea, 6Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam 463-707, Korea, 7Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea, 8These authors contributed equally to this work.
Correspondence to:*Correspondence: sjsj@dankook.ac.kr (SJ); iris1774@dankook.ac.kr (MYK)
Abstract
Since tenascin C is a factor expressed highly in the tu-mor-associated matrix, it would be a desirable first step for targeting the tumor-specific microenvironment. In fact, a high level of tenascin C expression has been re-ported in most solid tumors, including lung cancer, colon cancer and glioblastoma. Therefore, the targeted binding of tenascin C in tumor stroma would inhibit tumor metastasis by modulating cancer cell growth and migration. We isolated a peptide that bound to tenascin C by phage display peptide library selection, and the selected peptide specifically recognized tenascin C protein in xenograft mouse tissue. We also observed exclusive staining of tenascin C by the selected peptide in tumor patient tissues. Moreover, the peptide reduced tenascin C-induced cell rounding and migration. We propose that the tenascin C targeting peptide may be useful as a specific anti-cancer diagnostic and therapeutic tool for most human solid tumors.
Keywords: anti-cancer therapeutic, peptide, tenascin C
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