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Mol. Cells 2012; 33(1): 53-59

Published online January 31, 2012

https://doi.org/10.1007/s10059-012-2208-2

© The Korean Society for Molecular and Cellular Biology

Interspecies Signaling through QscR, a Quorum Receptor of Pseudomonas aeruginosa

Changwan Ha1, Seong Joon Park1, Su-Jin Im, Su-Jin Park, and Joon-Hee Lee*

Department of Pharmacy, College of Pharmacy, Pusan National University, Busan 609-735, Korea, 1These authors contributed equally to this work.

Correspondence to : *Correspondence: joonhee@pusan.ac.kr

Received: September 26, 2011; Revised: October 21, 2011; Accepted: October 24, 2011

Abstract

The QS machinery of Pseudomonas aeruginosa, an opportunistic human pathogen, consists of three acyl-homo-serine lactone (acyl-HSL) signaling systems, LasR-I, RhlR-I, and QscR. QscR, known as an orphan receptor and a repressor of other QS systems, operates its own regulon using N-3-oxododecanoyl HSL (3OC12), which is synthesized by LasI, as its signal. In this study, we addressed the role of QscR in interspecies communication. We found that QscR auto-activates its own transcription in the presence of 3OC12. In a single population of P. aeruginosa, where 3OC12 is the sole signal available for QscR, the QscR regulon is activated by 3OC12 produced by the LasI-R system. However, the broad signal specificity of QscR allowed it to respond to a non-P. aeruginosa signal, such as N-decanoyl HSL (C10) and N-3-hydroxydecanoyl HSL (3OHC10), which preferentially activated QscR to LasR. The signal extracts from Pseudomonas fluorescens and Burkholeria vietnamiensis also preferentially activated QscR. These non-P. aeruginosa signals activated QscR more strongly than 3OC12, the authentic P. aeruginosa signal. Since a variety of acyl-HSLs are produced in the multi-species habitat of nature, our study provides a clue for the particular situation that allows QscR to secede from the conventional QS cascade in mixed microbial community.

Keywords interspecies signaling, LasR, Pseudomonas aeruginosa, QscR, quorum

Article

Research Article

Mol. Cells 2012; 33(1): 53-59

Published online January 31, 2012 https://doi.org/10.1007/s10059-012-2208-2

Copyright © The Korean Society for Molecular and Cellular Biology.

Interspecies Signaling through QscR, a Quorum Receptor of Pseudomonas aeruginosa

Changwan Ha1, Seong Joon Park1, Su-Jin Im, Su-Jin Park, and Joon-Hee Lee*

Department of Pharmacy, College of Pharmacy, Pusan National University, Busan 609-735, Korea, 1These authors contributed equally to this work.

Correspondence to:*Correspondence: joonhee@pusan.ac.kr

Received: September 26, 2011; Revised: October 21, 2011; Accepted: October 24, 2011

Abstract

The QS machinery of Pseudomonas aeruginosa, an opportunistic human pathogen, consists of three acyl-homo-serine lactone (acyl-HSL) signaling systems, LasR-I, RhlR-I, and QscR. QscR, known as an orphan receptor and a repressor of other QS systems, operates its own regulon using N-3-oxododecanoyl HSL (3OC12), which is synthesized by LasI, as its signal. In this study, we addressed the role of QscR in interspecies communication. We found that QscR auto-activates its own transcription in the presence of 3OC12. In a single population of P. aeruginosa, where 3OC12 is the sole signal available for QscR, the QscR regulon is activated by 3OC12 produced by the LasI-R system. However, the broad signal specificity of QscR allowed it to respond to a non-P. aeruginosa signal, such as N-decanoyl HSL (C10) and N-3-hydroxydecanoyl HSL (3OHC10), which preferentially activated QscR to LasR. The signal extracts from Pseudomonas fluorescens and Burkholeria vietnamiensis also preferentially activated QscR. These non-P. aeruginosa signals activated QscR more strongly than 3OC12, the authentic P. aeruginosa signal. Since a variety of acyl-HSLs are produced in the multi-species habitat of nature, our study provides a clue for the particular situation that allows QscR to secede from the conventional QS cascade in mixed microbial community.

Keywords: interspecies signaling, LasR, Pseudomonas aeruginosa, QscR, quorum

Mol. Cells
Nov 30, 2022 Vol.45 No.11, pp. 763~867
COVER PICTURE
Naive (cyan) and axotomized (magenta) retinal ganglion cell axons in Xenopus tropicalis (Choi et al., pp. 846-854).

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