Mol. Cells 2011; 32(5): 451-457
Published online November 11, 2011
https://doi.org/10.1007/s10059-011-0137-0
© The Korean Society for Molecular and Cellular Biology
Correspondence to : *Correspondence: wangjh1@sj-hospital.org
Jolkinolide B, a bioactive diterpene isolated from the roots of Euphorbia fischeriana Steud, is known to induce apoptosis in cancer cells. However, the molecular mecha-nism of its anti-cancer activity have not been fully eluci-dated. In the present study, we found that Jolkinolide B reduced cell viability and induced apoptosis in a dose- and time-dependent manner in human leukemic U937. The induction of apoptosis was also accompanied by the downregulation of PI3K/Akt and the inhibitor of apoptosis protein (IAP) family proteins. Moreover, we observed that Jolkinolide B treatment resulted in activation of caspase-3 and -9, which may partly explain the anti-cancer activity of Jolkinolide B. Taken together, our study for the first time suggest that Jolkinolide B is able to enhance apoptosis of U937 cells, at least in part, through downregulation of PI3K/Akt and IAP family proteins. Moreover, the triggering of caspase-3 and -9 activation mediated apoptotic induction.
Keywords apoptosis, caspase, Euphorbia fischeriana Steud, Jolkinolide B, U937
Mol. Cells 2011; 32(5): 451-457
Published online November 30, 2011 https://doi.org/10.1007/s10059-011-0137-0
Copyright © The Korean Society for Molecular and Cellular Biology.
Jia-He Wang*, Yi-Jun Zhou1, Xue Bai, and Ping He
Department of Geriatrics, Shengjing Hospital of China Medical University, Shenyang, 110004, P.R. China, 1Department of Endocrinology and Metabolism, the fourth affiliated hospital of China Medical University, Shenyang, 110004, P.R. China
Correspondence to:*Correspondence: wangjh1@sj-hospital.org
Jolkinolide B, a bioactive diterpene isolated from the roots of Euphorbia fischeriana Steud, is known to induce apoptosis in cancer cells. However, the molecular mecha-nism of its anti-cancer activity have not been fully eluci-dated. In the present study, we found that Jolkinolide B reduced cell viability and induced apoptosis in a dose- and time-dependent manner in human leukemic U937. The induction of apoptosis was also accompanied by the downregulation of PI3K/Akt and the inhibitor of apoptosis protein (IAP) family proteins. Moreover, we observed that Jolkinolide B treatment resulted in activation of caspase-3 and -9, which may partly explain the anti-cancer activity of Jolkinolide B. Taken together, our study for the first time suggest that Jolkinolide B is able to enhance apoptosis of U937 cells, at least in part, through downregulation of PI3K/Akt and IAP family proteins. Moreover, the triggering of caspase-3 and -9 activation mediated apoptotic induction.
Keywords: apoptosis, caspase, Euphorbia fischeriana Steud, Jolkinolide B, U937
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