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Mol. Cells 2011; 32(6): 579-587

Published online December 31, 2011

https://doi.org/10.1007/s10059-011-0186-4

© The Korean Society for Molecular and Cellular Biology

Quantitative Trait Loci for Cold Tolerance of Rice Recombinant Inbred Lines in Low Temperature Environments

Wenzhu Jiang1,6, Yong-Mei Jin2,6, Joohyun Lee2, Kang-Ie Lee2, Rihua Piao2, Longzhi Han3, Jin-Chul Shin4, Rong-De Jin5, Tiehua Cao5, Hong-Yu Pan1, Xinglin Du1,*, and Hee-Jong Koh2,*

1College of Plant Science, Jilin University, Changchun, 130062, China, 2Department of Plant Science, Research Institute of Agriculture and Life Sciences, and Plant Genomics and Breeding Institute, Seoul National University, Seoul 151-921, Korea, 3Key Laboratory of Crop Germplasm Resources and Biotechnology, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China, 4National Institute of Crop Science, Rural Development Administration, Suwon 441-857, Korea, 5Jilin Academy of Agricultural Sciences, Changchun 130124, China, 6These authors contributed equally to this work.

Correspondence to : *Correspondence: heejkoh@snu.ac.kr (HJK); duxinglin2004@163.com (XD)

Received: September 5, 2011; Revised: September 25, 2011; Accepted: September 28, 2011

Abstract

Low temperature is one of the major environmental stress-es in rice cultivation in high-altitude and high-latitude regions. In this study, we cultivated a set of re-combinant inbred lines (RIL) derived from Dasanbyeo (indica) / TR22183 (japonica) crosses in Yanji (high-latitude area), Kunming (high-altitude area), Chuncheon (cold water irrigation) and Suwon (normal) to evaluate the main effects of quantitative trait loci (QTL) and epistatic QTL (E-QTL) with regard to their interactions with environments for cold-related traits. Six QTLs for spikelet fertility (SF) were identified in three cold treatment locations. Among them, four QTLs on chromosomes 2, 7, 8, and 10 were validated by several near isogenic lines (NILs) under cold treatment in Chuncheon. A total of 57 QTLs and 76 E-QTLs for nine cold-related traits were identified as distributing on all 12 chromosomes; among them, 19 QTLs and E-QTLs showed significant interactions of QTLs and envi-ronments (QEIs). The total phenotypic variation explained by each trait ranged from 13.2 to 29.1% in QTLs, 10.6 to 29.0% in E-QTLs, 2.2 to 8.8% in QEIs and 1.0% to 7.7% in E-QTL ? environment interactions (E-QEIs). These results demonstrate that epistatic effects and QEIs are important properties of QTL parameters for cold tolerance at the reproductive stage. In order to develop cold tolerant varieties adaptable to wide-ranges of cold stress, a strategy facilitating marker-assisted selection (MAS) is being adopted to accumulate QTLs identified from different environments.

Keywords cold tolerance, near isogenic line, quantitative trait loci, rice, RIL

Article

Research Article

Mol. Cells 2011; 32(6): 579-587

Published online December 31, 2011 https://doi.org/10.1007/s10059-011-0186-4

Copyright © The Korean Society for Molecular and Cellular Biology.

Quantitative Trait Loci for Cold Tolerance of Rice Recombinant Inbred Lines in Low Temperature Environments

Wenzhu Jiang1,6, Yong-Mei Jin2,6, Joohyun Lee2, Kang-Ie Lee2, Rihua Piao2, Longzhi Han3, Jin-Chul Shin4, Rong-De Jin5, Tiehua Cao5, Hong-Yu Pan1, Xinglin Du1,*, and Hee-Jong Koh2,*

1College of Plant Science, Jilin University, Changchun, 130062, China, 2Department of Plant Science, Research Institute of Agriculture and Life Sciences, and Plant Genomics and Breeding Institute, Seoul National University, Seoul 151-921, Korea, 3Key Laboratory of Crop Germplasm Resources and Biotechnology, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China, 4National Institute of Crop Science, Rural Development Administration, Suwon 441-857, Korea, 5Jilin Academy of Agricultural Sciences, Changchun 130124, China, 6These authors contributed equally to this work.

Correspondence to:*Correspondence: heejkoh@snu.ac.kr (HJK); duxinglin2004@163.com (XD)

Received: September 5, 2011; Revised: September 25, 2011; Accepted: September 28, 2011

Abstract

Low temperature is one of the major environmental stress-es in rice cultivation in high-altitude and high-latitude regions. In this study, we cultivated a set of re-combinant inbred lines (RIL) derived from Dasanbyeo (indica) / TR22183 (japonica) crosses in Yanji (high-latitude area), Kunming (high-altitude area), Chuncheon (cold water irrigation) and Suwon (normal) to evaluate the main effects of quantitative trait loci (QTL) and epistatic QTL (E-QTL) with regard to their interactions with environments for cold-related traits. Six QTLs for spikelet fertility (SF) were identified in three cold treatment locations. Among them, four QTLs on chromosomes 2, 7, 8, and 10 were validated by several near isogenic lines (NILs) under cold treatment in Chuncheon. A total of 57 QTLs and 76 E-QTLs for nine cold-related traits were identified as distributing on all 12 chromosomes; among them, 19 QTLs and E-QTLs showed significant interactions of QTLs and envi-ronments (QEIs). The total phenotypic variation explained by each trait ranged from 13.2 to 29.1% in QTLs, 10.6 to 29.0% in E-QTLs, 2.2 to 8.8% in QEIs and 1.0% to 7.7% in E-QTL ? environment interactions (E-QEIs). These results demonstrate that epistatic effects and QEIs are important properties of QTL parameters for cold tolerance at the reproductive stage. In order to develop cold tolerant varieties adaptable to wide-ranges of cold stress, a strategy facilitating marker-assisted selection (MAS) is being adopted to accumulate QTLs identified from different environments.

Keywords: cold tolerance, near isogenic line, quantitative trait loci, rice, RIL

Mol. Cells
Nov 30, 2022 Vol.45 No.11, pp. 763~867
COVER PICTURE
Naive (cyan) and axotomized (magenta) retinal ganglion cell axons in Xenopus tropicalis (Choi et al., pp. 846-854).

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