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Mol. Cells 2011; 32(6): 519-526
Published online November 9, 2011
https://doi.org/10.1007/s10059-011-0135-2
© The Korean Society for Molecular and Cellular Biology
Analysis of Arabidopsis Transcription Factor Families Revealed Extensive Capacity for Cell-to-Cell Movement as Well as Discrete Trafficking Patterns
Correspondence to : *Correspondence: kimjy@gnu.ac.kr
In plants, cell-to-cell communication is pivotal for the orchestration of cell fate determination, organ development, and the integration of whole plant physiology. One of the strategies for intercellular communication uses symplasmic communication channels, called plasmodesmata (PD). These PD establish unique cytoplasmic channels for the intercellular exchange not only of metabolites and small signaling molecules, but also of regulatory proteins and RNAs to allow for local orchestration of development and physiology. A number of non-cell-autonomous transcrip-tion factors (NCATFs) have been shown to function in the coordination of specific regulatory networks. To further explore the potential of such NCATFs, a genome-wide screen was performed on the transcription factor (TF) families in Arabidopsis. We here report that, among the 76 TFs examined, 22 were shown to move beyond their sites of transcription in the root apex; these NCATFs belonged to 17 TF families, including homeobox, GRAS, and MYB. Expression studies performed on variously-sized mCherry constructs identified a range of PD size exclusion limits within tissues of the root. In addition, our studies showed that actual protein level was an important factor controlling the range of TF intercellular movement. Interestingly, our studies on CAPRICE movement revealed tissue-speci-ficity with respect to the mode of intercellular trafficking. These findings are discussed with respect to the regulation between cell-autonomous or non-cell-autonomous action.
Keywords cell-to-cell communication, intercellular protein trafficking, non-cell-autonomous transcription factor (NCATF), plasmodesmata (PD)
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Research Article
Mol. Cells 2011; 32(6): 519-526
Published online December 31, 2011 https://doi.org/10.1007/s10059-011-0135-2
Copyright © The Korean Society for Molecular and Cellular Biology.
Analysis of Arabidopsis Transcription Factor Families Revealed Extensive Capacity for Cell-to-Cell Movement as Well as Discrete Trafficking Patterns
Yeonggil Rim1, Lijun Huang1, Hyosub Chu1,3, Xiao Han1, Won Kyong Cho1,4, Che Ok Jeon1,5, Hye Jin Kim1, Jong-Chan Hong1, William J. Lucas2, and Jae-Yean Kim1,*
1Division of Applied Life Science (BK21/WCU program), Gyeongsang National University, Jinju 660-701, Korea, 2Department of Plant Biology, College of Biological Sciences, University of California, Davis, CA 95616, USA, Present, 3Present address: Bioindustrial Process Center, Jeonbuk Branch Institute of Korea Research Institute of Bioscience and Biotechnology, Jeonbuk 580-185, Korea, 4Present address: Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea, 5Present address: Schools of Biological Sciences, Research Center for Biomolecules and Biosystems, Chung-Ang University, Seoul 156-756, Korea
Correspondence to:*Correspondence: kimjy@gnu.ac.kr
Abstract
In plants, cell-to-cell communication is pivotal for the orchestration of cell fate determination, organ development, and the integration of whole plant physiology. One of the strategies for intercellular communication uses symplasmic communication channels, called plasmodesmata (PD). These PD establish unique cytoplasmic channels for the intercellular exchange not only of metabolites and small signaling molecules, but also of regulatory proteins and RNAs to allow for local orchestration of development and physiology. A number of non-cell-autonomous transcrip-tion factors (NCATFs) have been shown to function in the coordination of specific regulatory networks. To further explore the potential of such NCATFs, a genome-wide screen was performed on the transcription factor (TF) families in Arabidopsis. We here report that, among the 76 TFs examined, 22 were shown to move beyond their sites of transcription in the root apex; these NCATFs belonged to 17 TF families, including homeobox, GRAS, and MYB. Expression studies performed on variously-sized mCherry constructs identified a range of PD size exclusion limits within tissues of the root. In addition, our studies showed that actual protein level was an important factor controlling the range of TF intercellular movement. Interestingly, our studies on CAPRICE movement revealed tissue-speci-ficity with respect to the mode of intercellular trafficking. These findings are discussed with respect to the regulation between cell-autonomous or non-cell-autonomous action.
Keywords: cell-to-cell communication, intercellular protein trafficking, non-cell-autonomous transcription factor (NCATF), plasmodesmata (PD)
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