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Mol. Cells 2011; 32(5): 445-450

Published online November 30, 2011

https://doi.org/10.1007/s10059-011-0132-5

© The Korean Society for Molecular and Cellular Biology

S100A2 Level Changes Are Related to Human Periodontitis

Sun-Hee Heo1, Young-Jin Choi1, Ji-Hyun Lee1, Jae-Mok Lee2,*, and Je-Yoel Cho1,*

1Department of Biochemistry, School of Dentistry, Kyungpook National University, Daegu 700-422, Korea, 2Department of Periodontology, School of Dentistry, Kyungpook National University, Daegu 700-412, Korea

Correspondence to : *Correspondence: jeycho@knu.ac.kr (JYC); leejm@knu.ac.kr (JML)

Received: June 21, 2011; Revised: August 21, 2011; Accepted: August 25, 2011

Abstract

Periodontitis is an inflammatory disease, which, when severe, can result in tooth loss, that affects the quality of life. S100A2 was previously identified as a component of gingival crevicular fluid (GCF) via proteome analysis, but it has not been investigated whether S100A2 plays a role in periodontitis. In this study, we analyzed mRNA expression of S100A2 in gingival tissues from normal and classified periodontal disease patients and compared it to that of S100A8 and S100A9. Quantitative real time-PCR revealed that the mRNA expression levels of S100A2, S100A8, and S100A9 were significantly upregulated in gingival tissues with gingivitis, moderate periodontitis, and severe periodontitis compared to normal tissues. In addition, S100A2 proteins in GCF and the conditioned media of lipopolysaccharide (LPS)-treated Jurkat cells were confirmed by ELISA. S100A2 protein levels were significantly higher in GCF in gingivitis and moderate periodontitis groups than in normal groups. S100A2 mRNA expression and protein secretion were also increased by LPS stimulation. Based on the up-regulation of S100A2 in LPS-stimulated immune cells, gingival tissues and GCF from periodontal disease groups, we conclude that S100A2 is a functional component in the immune response during periodontitis and may serve as a potential biomarker for periodontitis.

Keywords gingival crevicular fluid, gingival tissue, lipopolysaccharide, periodontitis, s100a2

Article

Research Article

Mol. Cells 2011; 32(5): 445-450

Published online November 30, 2011 https://doi.org/10.1007/s10059-011-0132-5

Copyright © The Korean Society for Molecular and Cellular Biology.

S100A2 Level Changes Are Related to Human Periodontitis

Sun-Hee Heo1, Young-Jin Choi1, Ji-Hyun Lee1, Jae-Mok Lee2,*, and Je-Yoel Cho1,*

1Department of Biochemistry, School of Dentistry, Kyungpook National University, Daegu 700-422, Korea, 2Department of Periodontology, School of Dentistry, Kyungpook National University, Daegu 700-412, Korea

Correspondence to:*Correspondence: jeycho@knu.ac.kr (JYC); leejm@knu.ac.kr (JML)

Received: June 21, 2011; Revised: August 21, 2011; Accepted: August 25, 2011

Abstract

Periodontitis is an inflammatory disease, which, when severe, can result in tooth loss, that affects the quality of life. S100A2 was previously identified as a component of gingival crevicular fluid (GCF) via proteome analysis, but it has not been investigated whether S100A2 plays a role in periodontitis. In this study, we analyzed mRNA expression of S100A2 in gingival tissues from normal and classified periodontal disease patients and compared it to that of S100A8 and S100A9. Quantitative real time-PCR revealed that the mRNA expression levels of S100A2, S100A8, and S100A9 were significantly upregulated in gingival tissues with gingivitis, moderate periodontitis, and severe periodontitis compared to normal tissues. In addition, S100A2 proteins in GCF and the conditioned media of lipopolysaccharide (LPS)-treated Jurkat cells were confirmed by ELISA. S100A2 protein levels were significantly higher in GCF in gingivitis and moderate periodontitis groups than in normal groups. S100A2 mRNA expression and protein secretion were also increased by LPS stimulation. Based on the up-regulation of S100A2 in LPS-stimulated immune cells, gingival tissues and GCF from periodontal disease groups, we conclude that S100A2 is a functional component in the immune response during periodontitis and may serve as a potential biomarker for periodontitis.

Keywords: gingival crevicular fluid, gingival tissue, lipopolysaccharide, periodontitis, s100a2

Mol. Cells
Jan 31, 2023 Vol.46 No.1, pp. 1~67
COVER PICTURE
RNAs form diverse shapes and play multiple functions as central molecules of gene expression. In this special issue on RNA, seven minireviews illustrate how basic concepts and recent RNA biology findings are transformed into new and exciting RNA therapeutics.

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