Mol. Cells 2011; 32(3): 243-249
Published online August 29, 2011
https://doi.org/10.1007/s10059-011-1037-z
© The Korean Society for Molecular and Cellular Biology
Seunghee Bae1, Eun-Mee Lee1, Hwa Jun Cha1, Karam Kim1, Yeongmin Yoon1,2, Hyunjin Lee1,2, Jongran Kim1,2, Yu-Jeong Kim3, Hong Ghi Lee4, Hoi-Kyung Jeung5, Yoo Hong Min5, and Sungkwan An1,2,*
Correspondence to : *Correspondence: ansfgrc@konkuk.ac.kr
Resveratrol is a plant phenolic phytoalexin that has been reported to have antitumor properties in several types of cancers. In particular, several studies have suggested that resveratrol exerts antiproliferative effects against A549 human non-small cell lung cancer cells; however, its me-chanism of action remains incompletely understood. Deregulation of microRNAs (miRNAs), a class of small, non-coding, regulatory RNA molecules involved in gene expression, is strongly correlated with lung cancer. In this study, we demonstrated that resveratrol treatment altered miRNA expression in A549 cells. Using microarray analysis, we identified 71 miRNAs exhibiting greater than 2-fold expression changes in resveratrol-treated cells relative to their expression levels in untreated cells. Furthermore, we identified target genes related to apoptosis, cell cycle regulation, cell proliferation, and differentiation using a miRNA target-prediction program. In conclusion, our data demonstrate that resveratrol induces considerable changes in the miRNA expression profiles of A549 cells, suggesting a novel approach for studying the anticancer mechanisms of resveratrol.
Keywords A549, human non-small cell lung cancer cells, microRNA, resveratrol
Mol. Cells 2011; 32(3): 243-249
Published online September 30, 2011 https://doi.org/10.1007/s10059-011-1037-z
Copyright © The Korean Society for Molecular and Cellular Biology.
Seunghee Bae1, Eun-Mee Lee1, Hwa Jun Cha1, Karam Kim1, Yeongmin Yoon1,2, Hyunjin Lee1,2, Jongran Kim1,2, Yu-Jeong Kim3, Hong Ghi Lee4, Hoi-Kyung Jeung5, Yoo Hong Min5, and Sungkwan An1,2,*
1Functional Genoproteome Research Centre, Konkuk University, Seoul 143-701, Korea, 2LIFEnGENE, Inc., Konkuk University, Seoul 143-701, Korea, 3Department of Beauty Design, Yeoju Institute of Technology, Yeoju 469-705, Korea, 4Department of Internal Medicine, Konkuk University Medical Center, Konkuk University School of Medicine, Seoul 143-729, Korea, 5Department of Internal Medicine and Center for Chronic Metabolic Disease, Yonsei University College of Medicine, Seoul 102-752, Korea
Correspondence to:*Correspondence: ansfgrc@konkuk.ac.kr
Resveratrol is a plant phenolic phytoalexin that has been reported to have antitumor properties in several types of cancers. In particular, several studies have suggested that resveratrol exerts antiproliferative effects against A549 human non-small cell lung cancer cells; however, its me-chanism of action remains incompletely understood. Deregulation of microRNAs (miRNAs), a class of small, non-coding, regulatory RNA molecules involved in gene expression, is strongly correlated with lung cancer. In this study, we demonstrated that resveratrol treatment altered miRNA expression in A549 cells. Using microarray analysis, we identified 71 miRNAs exhibiting greater than 2-fold expression changes in resveratrol-treated cells relative to their expression levels in untreated cells. Furthermore, we identified target genes related to apoptosis, cell cycle regulation, cell proliferation, and differentiation using a miRNA target-prediction program. In conclusion, our data demonstrate that resveratrol induces considerable changes in the miRNA expression profiles of A549 cells, suggesting a novel approach for studying the anticancer mechanisms of resveratrol.
Keywords: A549, human non-small cell lung cancer cells, microRNA, resveratrol
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