Byung Joo Kim" /> Byung Joo Kim, Joo Hyun Nam, and Seon Jeong Kim*

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. Mol. Cells 2011;32:153-60. https://doi.org/10.1007/s10059-011-1019-1">
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Mol. Cells 2011; 32(2): 153-160

Published online August 31, 2011

https://doi.org/10.1007/s10059-011-1019-1

© The Korean Society for Molecular and Cellular Biology

Effects of Transient Receptor Potential Channel Blockers on Pacemaker Activity in Interstitial Cells of Cajal from Mouse Small Intestine

Byung Joo Kim1,4, Joo Hyun Nam2,4, and Seon Jeong Kim3,*

1Division of Longevity and Biofunctional Medicine, Pusan National University School of Korean Medicine, Yangsan 626-870, Korea, 2Department of Physiology, Dongguk University College of Medicine, Kyungju 780-714, Korea, 3Center for Bio-Artificial Muscle and Department of Biomedical Engineering, Hanyang University, Seoul 133-791, Korea, 4These authors contributed equally to this work.

Correspondence to : *Correspondence: sjk@hanyang.ac.kr

Received: January 26, 2011; Revised: April 25, 2011; Accepted: May 2, 2011

Abstract

The interstitial cells of Cajal (ICCs) are pacemakers in the gastrointestinal tract and transient receptor potential melastatin type 7 (TRPM7) is a candidate for pacemaker channels. The effect of the 5-lipoxygenase (5-LOX) inhibitors NDGA, AA861, MK886 and zileuton on pacemaking activity of ICCs was examined using the whole cell patch clamp technique. NDGA and AA861 decreased the amplitude of pacemaker potentials in ICC clusters, but the resting membrane potentials displayed little change, respectively. Also, perfusing NDGA and AA861 into the bath reduced both inward current and outward current in TRPM7-like current in single ICC, respectively. But, they had no effects on Ca2+ activated Cl- currents. The 5-LOX inhibitors MK886 and zileuton were, however, ineffective in pacemaker potentials in ICC clusters and in TRPM7-like current in single ICC, respectively. A specific TRPC3 inhibitor, pyrazole compound (Pyr3), and a specific TRPM4 inhibitor, 9-phenanthrol, had no effects in pacemaker potentials in ICC clusters and in TRPM7-like current in single ICC. These results suggest that, among the tested 5-LOX inhibitors, NDGA and AA861 modulate the pacemaker activities of the ICCs, and that the TRPM7 channel can affect intestinal motility.

Keywords 5-lipoxygenase inhibitor, interstitial cells of Cajal, pacemaker, TRPM7

Article

Research Article

Mol. Cells 2011; 32(2): 153-160

Published online August 31, 2011 https://doi.org/10.1007/s10059-011-1019-1

Copyright © The Korean Society for Molecular and Cellular Biology.

Effects of Transient Receptor Potential Channel Blockers on Pacemaker Activity in Interstitial Cells of Cajal from Mouse Small Intestine

Byung Joo Kim1,4, Joo Hyun Nam2,4, and Seon Jeong Kim3,*

1Division of Longevity and Biofunctional Medicine, Pusan National University School of Korean Medicine, Yangsan 626-870, Korea, 2Department of Physiology, Dongguk University College of Medicine, Kyungju 780-714, Korea, 3Center for Bio-Artificial Muscle and Department of Biomedical Engineering, Hanyang University, Seoul 133-791, Korea, 4These authors contributed equally to this work.

Correspondence to:*Correspondence: sjk@hanyang.ac.kr

Received: January 26, 2011; Revised: April 25, 2011; Accepted: May 2, 2011

Abstract

The interstitial cells of Cajal (ICCs) are pacemakers in the gastrointestinal tract and transient receptor potential melastatin type 7 (TRPM7) is a candidate for pacemaker channels. The effect of the 5-lipoxygenase (5-LOX) inhibitors NDGA, AA861, MK886 and zileuton on pacemaking activity of ICCs was examined using the whole cell patch clamp technique. NDGA and AA861 decreased the amplitude of pacemaker potentials in ICC clusters, but the resting membrane potentials displayed little change, respectively. Also, perfusing NDGA and AA861 into the bath reduced both inward current and outward current in TRPM7-like current in single ICC, respectively. But, they had no effects on Ca2+ activated Cl- currents. The 5-LOX inhibitors MK886 and zileuton were, however, ineffective in pacemaker potentials in ICC clusters and in TRPM7-like current in single ICC, respectively. A specific TRPC3 inhibitor, pyrazole compound (Pyr3), and a specific TRPM4 inhibitor, 9-phenanthrol, had no effects in pacemaker potentials in ICC clusters and in TRPM7-like current in single ICC. These results suggest that, among the tested 5-LOX inhibitors, NDGA and AA861 modulate the pacemaker activities of the ICCs, and that the TRPM7 channel can affect intestinal motility.

Keywords: 5-lipoxygenase inhibitor, interstitial cells of Cajal, pacemaker, TRPM7

Mol. Cells
Aug 31, 2022 Vol.45 No.8, pp. 513~602
COVER PICTURE
Cryo-EM structure of human porphyrin transporter ABCB6 (main figure) shows that binding of hemin (inset, magenta) in concert with two glutathione molecules (cyan) primes ABCB6 for high ATP turnover (Kim et al., pp. 575-587).

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