Woo Sik Yu" /> Eunjung Ko" /> Hyo-Jeong Lee" /> Hyeung-Jin Jang" /> Woo Sik Yu, Soo-Jin Jeong, Ji-Hyun Kim, Hyo-Jung Lee, Hyo Sook Song, Min-Seok Kim, Eunjung Ko, Hyo-Jeong Lee, Jae-Ho Khil, Hyeung-Jin Jang, Young Chul Kim, Hyunsu Bae, Chang Yan Chen, and Sung-Hoon Kim*" /> Woo Sik Yu, Soo-Jin Jeong, Ji-Hyun Kim, Hyo-Jung Lee, Hyo Sook Song, Min-Seok Kim, Eunjung Ko, Hyo-Jeong Lee, Jae-Ho Khil, Hyeung-Jin Jang, Young Chul Kim, Hyunsu Bae, Chang Yan Chen, and Sung-Hoon Kim*. Mol. Cells 2011;32:123-32. https://doi.org/10.1007/s10059-011-2254-1">
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Mol. Cells 2011; 32(2): 123-132

Published online May 24, 2011

https://doi.org/10.1007/s10059-011-2254-1

© The Korean Society for Molecular and Cellular Biology

The Genome-Wide Expression Profile of 1,2,3,4,6-Penta-O-Galloyl-β-D-Glucose-Treated MDA-MB-231 Breast Cancer Cells: Molecular Target on Cancer Metabolism

Woo Sik Yu1,5, Soo-Jin Jeong1,5, Ji-Hyun Kim1, Hyo-Jung Lee1, Hyo Sook Song1, Min-Seok Kim2, Eunjung Ko1, Hyo-Jeong Lee1, Jae-Ho Khil3, Hyeung-Jin Jang1, Young Chul Kim1, Hyunsu Bae1, Chang Yan Chen4, and Sung-Hoon Kim1,*

Author Info. +

1Cancer Preventive Material Development Research Center, College of Oriental Medicine, Kyung Hee University, Seoul 130-701, Korea, 2College of Dental Medicine, Tufts University, Boston, USA, 3College of Physical Education, Kyung Hee University, Seoul 130-701, Korea, 4Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, USA, 5These authors contributed equally to this work.

Correspondence to : *Correspondence: sungkim7@khu.ac.kr

Received: October 11, 2011; Revised: March 26, 2011; Accepted: April 28, 2011

Abstract

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1,2,3,4,6-penta-O-galloyl-beta-D-glucose (PGG), a polyphe-nolic compound isolated from Rhus chinensis Mill. PGG has been known to have anti-tumor, anti-angiogenic and anti-diabetic activities. The present study revealed another underlying molecular target of PGG in MDA-MB-231 breast cancer cells by using Illumina Human Ref-8 expression BeadChip assay. Through the Beadstudio v3 micro assay program to compare the identified genes expressed in PGG-treated MDA-MB-231 cells with untreated control, we found several unique genes that are closely associated with pyruvate metabolism, glycolysis/gluconeogenesis and tyrosine metabolism, including PC, ACSS2, ACACA, ACYP2, ALDH3B1, FBP1, PRMT2 and COMT. Consistent with microarray data, real-time RT-PCR confirmed the sig-nificant down-regulation of these genes at mRNA level in PGG-treated MDA-MB-231 cells. Our findings suggest the potential of PGG as anticancer agent for breast cancer cells by targeting cancer metabolism genes.

Keywords cancer metabolism, MDA-MB-231, microarray, PGG, real-time PCR

Article

Research Article

Mol. Cells 2011; 32(2): 123-132

Published online August 31, 2011 https://doi.org/10.1007/s10059-011-2254-1

Copyright © The Korean Society for Molecular and Cellular Biology.

The Genome-Wide Expression Profile of 1,2,3,4,6-Penta-O-Galloyl-β-D-Glucose-Treated MDA-MB-231 Breast Cancer Cells: Molecular Target on Cancer Metabolism

Woo Sik Yu1,5, Soo-Jin Jeong1,5, Ji-Hyun Kim1, Hyo-Jung Lee1, Hyo Sook Song1, Min-Seok Kim2, Eunjung Ko1, Hyo-Jeong Lee1, Jae-Ho Khil3, Hyeung-Jin Jang1, Young Chul Kim1, Hyunsu Bae1, Chang Yan Chen4, and Sung-Hoon Kim1,*

1Cancer Preventive Material Development Research Center, College of Oriental Medicine, Kyung Hee University, Seoul 130-701, Korea, 2College of Dental Medicine, Tufts University, Boston, USA, 3College of Physical Education, Kyung Hee University, Seoul 130-701, Korea, 4Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, USA, 5These authors contributed equally to this work.

Correspondence to:*Correspondence: sungkim7@khu.ac.kr

Received: October 11, 2011; Revised: March 26, 2011; Accepted: April 28, 2011

Abstract

1,2,3,4,6-penta-O-galloyl-beta-D-glucose (PGG), a polyphe-nolic compound isolated from Rhus chinensis Mill. PGG has been known to have anti-tumor, anti-angiogenic and anti-diabetic activities. The present study revealed another underlying molecular target of PGG in MDA-MB-231 breast cancer cells by using Illumina Human Ref-8 expression BeadChip assay. Through the Beadstudio v3 micro assay program to compare the identified genes expressed in PGG-treated MDA-MB-231 cells with untreated control, we found several unique genes that are closely associated with pyruvate metabolism, glycolysis/gluconeogenesis and tyrosine metabolism, including PC, ACSS2, ACACA, ACYP2, ALDH3B1, FBP1, PRMT2 and COMT. Consistent with microarray data, real-time RT-PCR confirmed the sig-nificant down-regulation of these genes at mRNA level in PGG-treated MDA-MB-231 cells. Our findings suggest the potential of PGG as anticancer agent for breast cancer cells by targeting cancer metabolism genes.

Keywords: cancer metabolism, MDA-MB-231, microarray, PGG, real-time PCR

Mol. Cells
Nov 30, 2023 Vol.46 No.11, pp. 655~725
COVER PICTURE
Kim et al. (pp. 710-724) demonstrated that a pathogen-derived Ralstonia pseudosolanacearum type III effector RipL delays flowering time and enhances susceptibility to bacterial infection in Arabidopsis thaliana. Shown is the RipL-expressing Arabidopsis plant, which displays general dampening of the transcriptional program during pathogen infection, grown in long-day conditions.
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