Woo Sik Yu" /> Eunjung Ko" /> Hyo-Jeong Lee" /> Hyeung-Jin Jang" /> Woo Sik Yu, Soo-Jin Jeong, Ji-Hyun Kim, Hyo-Jung Lee, Hyo Sook Song, Min-Seok Kim, Eunjung Ko, Hyo-Jeong Lee, Jae-Ho Khil, Hyeung-Jin Jang, Young Chul Kim, Hyunsu Bae, Chang Yan Chen, and Sung-Hoon Kim*" /> Woo Sik Yu, Soo-Jin Jeong, Ji-Hyun Kim, Hyo-Jung Lee, Hyo Sook Song, Min-Seok Kim, Eunjung Ko, Hyo-Jeong Lee, Jae-Ho Khil, Hyeung-Jin Jang, Young Chul Kim, Hyunsu Bae, Chang Yan Chen, and Sung-Hoon Kim*. Mol. Cells 2011;32:123-32. https://doi.org/10.1007/s10059-011-2254-1">
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Mol. Cells 2011; 32(2): 123-132

Published online May 24, 2011

https://doi.org/10.1007/s10059-011-2254-1

© The Korean Society for Molecular and Cellular Biology

The Genome-Wide Expression Profile of 1,2,3,4,6-Penta-O-Galloyl-β-D-Glucose-Treated MDA-MB-231 Breast Cancer Cells: Molecular Target on Cancer Metabolism

Woo Sik Yu1,5, Soo-Jin Jeong1,5, Ji-Hyun Kim1, Hyo-Jung Lee1, Hyo Sook Song1, Min-Seok Kim2, Eunjung Ko1, Hyo-Jeong Lee1, Jae-Ho Khil3, Hyeung-Jin Jang1, Young Chul Kim1, Hyunsu Bae1, Chang Yan Chen4, and Sung-Hoon Kim1,*

Author Info. +

1Cancer Preventive Material Development Research Center, College of Oriental Medicine, Kyung Hee University, Seoul 130-701, Korea, 2College of Dental Medicine, Tufts University, Boston, USA, 3College of Physical Education, Kyung Hee University, Seoul 130-701, Korea, 4Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, USA, 5These authors contributed equally to this work.

Correspondence to : *Correspondence: sungkim7@khu.ac.kr

Received: October 11, 2011; Revised: March 26, 2011; Accepted: April 28, 2011

Abstract

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1,2,3,4,6-penta-O-galloyl-beta-D-glucose (PGG), a polyphe-nolic compound isolated from Rhus chinensis Mill. PGG has been known to have anti-tumor, anti-angiogenic and anti-diabetic activities. The present study revealed another underlying molecular target of PGG in MDA-MB-231 breast cancer cells by using Illumina Human Ref-8 expression BeadChip assay. Through the Beadstudio v3 micro assay program to compare the identified genes expressed in PGG-treated MDA-MB-231 cells with untreated control, we found several unique genes that are closely associated with pyruvate metabolism, glycolysis/gluconeogenesis and tyrosine metabolism, including PC, ACSS2, ACACA, ACYP2, ALDH3B1, FBP1, PRMT2 and COMT. Consistent with microarray data, real-time RT-PCR confirmed the sig-nificant down-regulation of these genes at mRNA level in PGG-treated MDA-MB-231 cells. Our findings suggest the potential of PGG as anticancer agent for breast cancer cells by targeting cancer metabolism genes.

Keywords cancer metabolism, MDA-MB-231, microarray, PGG, real-time PCR

Article

Research Article

Mol. Cells 2011; 32(2): 123-132

Published online August 31, 2011 https://doi.org/10.1007/s10059-011-2254-1

Copyright © The Korean Society for Molecular and Cellular Biology.

The Genome-Wide Expression Profile of 1,2,3,4,6-Penta-O-Galloyl-β-D-Glucose-Treated MDA-MB-231 Breast Cancer Cells: Molecular Target on Cancer Metabolism

Woo Sik Yu1,5, Soo-Jin Jeong1,5, Ji-Hyun Kim1, Hyo-Jung Lee1, Hyo Sook Song1, Min-Seok Kim2, Eunjung Ko1, Hyo-Jeong Lee1, Jae-Ho Khil3, Hyeung-Jin Jang1, Young Chul Kim1, Hyunsu Bae1, Chang Yan Chen4, and Sung-Hoon Kim1,*

1Cancer Preventive Material Development Research Center, College of Oriental Medicine, Kyung Hee University, Seoul 130-701, Korea, 2College of Dental Medicine, Tufts University, Boston, USA, 3College of Physical Education, Kyung Hee University, Seoul 130-701, Korea, 4Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, USA, 5These authors contributed equally to this work.

Correspondence to:*Correspondence: sungkim7@khu.ac.kr

Received: October 11, 2011; Revised: March 26, 2011; Accepted: April 28, 2011

Abstract

1,2,3,4,6-penta-O-galloyl-beta-D-glucose (PGG), a polyphe-nolic compound isolated from Rhus chinensis Mill. PGG has been known to have anti-tumor, anti-angiogenic and anti-diabetic activities. The present study revealed another underlying molecular target of PGG in MDA-MB-231 breast cancer cells by using Illumina Human Ref-8 expression BeadChip assay. Through the Beadstudio v3 micro assay program to compare the identified genes expressed in PGG-treated MDA-MB-231 cells with untreated control, we found several unique genes that are closely associated with pyruvate metabolism, glycolysis/gluconeogenesis and tyrosine metabolism, including PC, ACSS2, ACACA, ACYP2, ALDH3B1, FBP1, PRMT2 and COMT. Consistent with microarray data, real-time RT-PCR confirmed the sig-nificant down-regulation of these genes at mRNA level in PGG-treated MDA-MB-231 cells. Our findings suggest the potential of PGG as anticancer agent for breast cancer cells by targeting cancer metabolism genes.

Keywords: cancer metabolism, MDA-MB-231, microarray, PGG, real-time PCR

Mol. Cells
May 31, 2023 Vol.46 No.5, pp. 259~328
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The alpha-helices in the lamin filaments are depicted as coils, with different subdomains distinguished by various colors. Coil 1a is represented by magenta, coil 1b by yellow, L2 by green, coil 2a by white, coil 2b by brown, stutter by cyan, coil 2c by dark blue, and the lamin Ig-like domain by grey. In the background, cells are displayed, with the cytosol depicted in green and the nucleus in blue (Ahn et al., pp. 309-318).
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