Young-Saeng Jang" /> Young-Saeng Jang, Jae-Hee Kim, Goo-Young Seo, and Pyeung-Hyeun Kim*

" /> Young-Saeng Jang, Jae-Hee Kim, Goo-Young Seo, and Pyeung-Hyeun Kim*

. Mol. Cells 2011;32:251-5. https://doi.org/10.1007/s10059-011-1040-4">
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Mol. Cells 2011; 32(3): 251-255

Published online June 23, 2011

https://doi.org/10.1007/s10059-011-1040-4

© The Korean Society for Molecular and Cellular Biology

TGF-β1 Stimulates Mouse Macrophages to Express APRIL through Smad and p38MAPK/CREB Pathways

Young-Saeng Jang1, Jae-Hee Kim1, Goo-Young Seo1, and Pyeung-Hyeun Kim1,2,*

1Department of Molecular Bioscience, College of Biomedical Science, Chuncheon 200-701, Korea, 2Medical and Bio-Material Research Center, Kangwon National University, Chuncheon 200-701, Korea

Correspondence to : *Correspondence: phkim@kangwon.ac.kr

Received: March 7, 2011; Revised: May 26, 2011; Accepted: May 30, 2011

Abstract

A proliferation-inducing ligand (APRIL), a new TNF fam-ily member, supports B-cell survival and tumor cell proliferation. APRIL is secreted as a soluble protein by macrophages, dendritic cells and activated T cells. However, factors involved in regulation of APRIL expression are as yet unknown. In this study, we investigated the effect of TGF-β1 on APRIL expression in P388D1, a mouse macrophage cell line. TGF-β1 induced APRIL mRNA expression in a time- and dose-dependent manner. One nanogram per milliliter of TGF-β1 was optimal and APRIL transcripts appeared as early as 3 h after stimulation. Based on our studies, which included overexpression of Smad3, DN-Smad3, and sh-Smad3, we found that Smad3 mediates APRIL transcription at least partially. Further, experiments using inhibitors revealed that p38MAPK and CREB are also involved in TGF-β1-induced APRIL expression. These results suggest that TGF-β1, through Smad3 and p38MAPK/ CREB signaling pathways, stimulates APRIL expression in macrophages.

Keywords APRIL, CREB, p38 MAPK, Smad3/4, TGF-β1

Article

Research Article

Mol. Cells 2011; 32(3): 251-255

Published online September 30, 2011 https://doi.org/10.1007/s10059-011-1040-4

Copyright © The Korean Society for Molecular and Cellular Biology.

TGF-β1 Stimulates Mouse Macrophages to Express APRIL through Smad and p38MAPK/CREB Pathways

Young-Saeng Jang1, Jae-Hee Kim1, Goo-Young Seo1, and Pyeung-Hyeun Kim1,2,*

1Department of Molecular Bioscience, College of Biomedical Science, Chuncheon 200-701, Korea, 2Medical and Bio-Material Research Center, Kangwon National University, Chuncheon 200-701, Korea

Correspondence to:*Correspondence: phkim@kangwon.ac.kr

Received: March 7, 2011; Revised: May 26, 2011; Accepted: May 30, 2011

Abstract

A proliferation-inducing ligand (APRIL), a new TNF fam-ily member, supports B-cell survival and tumor cell proliferation. APRIL is secreted as a soluble protein by macrophages, dendritic cells and activated T cells. However, factors involved in regulation of APRIL expression are as yet unknown. In this study, we investigated the effect of TGF-β1 on APRIL expression in P388D1, a mouse macrophage cell line. TGF-β1 induced APRIL mRNA expression in a time- and dose-dependent manner. One nanogram per milliliter of TGF-β1 was optimal and APRIL transcripts appeared as early as 3 h after stimulation. Based on our studies, which included overexpression of Smad3, DN-Smad3, and sh-Smad3, we found that Smad3 mediates APRIL transcription at least partially. Further, experiments using inhibitors revealed that p38MAPK and CREB are also involved in TGF-β1-induced APRIL expression. These results suggest that TGF-β1, through Smad3 and p38MAPK/ CREB signaling pathways, stimulates APRIL expression in macrophages.

Keywords: APRIL, CREB, p38 MAPK, Smad3/4, TGF-β1

Mol. Cells
Jan 31, 2023 Vol.46 No.1, pp. 1~67
COVER PICTURE
RNAs form diverse shapes and play multiple functions as central molecules of gene expression. In this special issue on RNA, seven minireviews illustrate how basic concepts and recent RNA biology findings are transformed into new and exciting RNA therapeutics.

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