Young-Chul Choi" /> Young-Chul Choi, Sena Yoon, Yongsu Jeong, Jaeseung Yoon, and Kwanghee Baek*

" /> Young-Chul Choi, Sena Yoon, Yongsu Jeong, Jaeseung Yoon, and Kwanghee Baek*

. Mol. Cells 2011;32:77-82. https://doi.org/10.1007/s10059-011-1042-2">
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Mol. Cells 2011; 32(1): 77-82

Published online May 2, 2011

https://doi.org/10.1007/s10059-011-1042-2

© The Korean Society for Molecular and Cellular Biology

Regulation of Vascular Endothelial Growth Factor Signaling by miR-200b

Young-Chul Choi1, Sena Yoon1, Yongsu Jeong, Jaeseung Yoon, and Kwanghee Baek*

Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea, 1These authors contributed equally to this work.

Correspondence to : *Correspondence: khbaek@khu.ac.kr

Received: March 8, 2011; Revised: March 30, 2011; Accepted: March 31, 2011

Abstract

Vascular endothelial growth factor (VEGF) signaling plays an important role in angiogenesis. In the VEGF signaling pathway, the key components are VEGF and its receptors, Flt-1 and KDR. In this study, we show that transfection of synthetic miR-200b reduced protein levels of VEGF, Flt-1, and KDR. In A549 cells, miR-200b targeted the predicted binding sites in the 3?-untranslated region (3?-UTR) of VEGF, Flt-1, and KDR as revealed by a luciferase reporter assay. When transfected with miR-200b, the ability of HUVECs to form a capillary tube on Matrigel and VEGF-in-duced phosphorylation of ERK1/2 were significantly reduced. Taken together, these results suggest that miR-200b negatively regulates VEGF signaling by targeting VEGF and its receptors and that miR-200b may have therapeutic potential as an angiogenesis inhibitor.

Keywords Flt-1, KDR, microRNA, miR-200b, VEGF, VEGF signaling

Article

Research Article

Mol. Cells 2011; 32(1): 77-82

Published online July 31, 2011 https://doi.org/10.1007/s10059-011-1042-2

Copyright © The Korean Society for Molecular and Cellular Biology.

Regulation of Vascular Endothelial Growth Factor Signaling by miR-200b

Young-Chul Choi1, Sena Yoon1, Yongsu Jeong, Jaeseung Yoon, and Kwanghee Baek*

Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea, 1These authors contributed equally to this work.

Correspondence to:*Correspondence: khbaek@khu.ac.kr

Received: March 8, 2011; Revised: March 30, 2011; Accepted: March 31, 2011

Abstract

Vascular endothelial growth factor (VEGF) signaling plays an important role in angiogenesis. In the VEGF signaling pathway, the key components are VEGF and its receptors, Flt-1 and KDR. In this study, we show that transfection of synthetic miR-200b reduced protein levels of VEGF, Flt-1, and KDR. In A549 cells, miR-200b targeted the predicted binding sites in the 3?-untranslated region (3?-UTR) of VEGF, Flt-1, and KDR as revealed by a luciferase reporter assay. When transfected with miR-200b, the ability of HUVECs to form a capillary tube on Matrigel and VEGF-in-duced phosphorylation of ERK1/2 were significantly reduced. Taken together, these results suggest that miR-200b negatively regulates VEGF signaling by targeting VEGF and its receptors and that miR-200b may have therapeutic potential as an angiogenesis inhibitor.

Keywords: Flt-1, KDR, microRNA, miR-200b, VEGF, VEGF signaling

Mol. Cells
May 31, 2023 Vol.46 No.5, pp. 259~328
COVER PICTURE
The alpha-helices in the lamin filaments are depicted as coils, with different subdomains distinguished by various colors. Coil 1a is represented by magenta, coil 1b by yellow, L2 by green, coil 2a by white, coil 2b by brown, stutter by cyan, coil 2c by dark blue, and the lamin Ig-like domain by grey. In the background, cells are displayed, with the cytosol depicted in green and the nucleus in blue (Ahn et al., pp. 309-318).

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