Mol. Cells 2011; 32(1): 77-82
Published online May 2, 2011
https://doi.org/10.1007/s10059-011-1042-2
© The Korean Society for Molecular and Cellular Biology
Young-Chul Choi1, Sena Yoon1, Yongsu Jeong, Jaeseung Yoon, and Kwanghee Baek*
Correspondence to : *Correspondence: khbaek@khu.ac.kr
Vascular endothelial growth factor (VEGF) signaling plays an important role in angiogenesis. In the VEGF signaling pathway, the key components are VEGF and its receptors, Flt-1 and KDR. In this study, we show that transfection of synthetic miR-200b reduced protein levels of VEGF, Flt-1, and KDR. In A549 cells, miR-200b targeted the predicted binding sites in the 3?-untranslated region (3?-UTR) of VEGF, Flt-1, and KDR as revealed by a luciferase reporter assay. When transfected with miR-200b, the ability of HUVECs to form a capillary tube on Matrigel and VEGF-in-duced phosphorylation of ERK1/2 were significantly reduced. Taken together, these results suggest that miR-200b negatively regulates VEGF signaling by targeting VEGF and its receptors and that miR-200b may have therapeutic potential as an angiogenesis inhibitor.
Keywords Flt-1, KDR, microRNA, miR-200b, VEGF, VEGF signaling
Mol. Cells 2011; 32(1): 77-82
Published online July 31, 2011 https://doi.org/10.1007/s10059-011-1042-2
Copyright © The Korean Society for Molecular and Cellular Biology.
Young-Chul Choi1, Sena Yoon1, Yongsu Jeong, Jaeseung Yoon, and Kwanghee Baek*
Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea, 1These authors contributed equally to this work.
Correspondence to:*Correspondence: khbaek@khu.ac.kr
Vascular endothelial growth factor (VEGF) signaling plays an important role in angiogenesis. In the VEGF signaling pathway, the key components are VEGF and its receptors, Flt-1 and KDR. In this study, we show that transfection of synthetic miR-200b reduced protein levels of VEGF, Flt-1, and KDR. In A549 cells, miR-200b targeted the predicted binding sites in the 3?-untranslated region (3?-UTR) of VEGF, Flt-1, and KDR as revealed by a luciferase reporter assay. When transfected with miR-200b, the ability of HUVECs to form a capillary tube on Matrigel and VEGF-in-duced phosphorylation of ERK1/2 were significantly reduced. Taken together, these results suggest that miR-200b negatively regulates VEGF signaling by targeting VEGF and its receptors and that miR-200b may have therapeutic potential as an angiogenesis inhibitor.
Keywords: Flt-1, KDR, microRNA, miR-200b, VEGF, VEGF signaling
Hyeonseo Hwang, Hee Ryung Chang, and Daehyun Baek
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