Young-Chul Choi" /> Young-Chul Choi, Sena Yoon, Yongsu Jeong, Jaeseung Yoon, and Kwanghee Baek*

" /> Young-Chul Choi, Sena Yoon, Yongsu Jeong, Jaeseung Yoon, and Kwanghee Baek*

. Mol. Cells 2011;32:77-82. https://doi.org/10.1007/s10059-011-1042-2">
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Mol. Cells 2011; 32(1): 77-82

Published online May 2, 2011

https://doi.org/10.1007/s10059-011-1042-2

© The Korean Society for Molecular and Cellular Biology

Regulation of Vascular Endothelial Growth Factor Signaling by miR-200b

Young-Chul Choi1, Sena Yoon1, Yongsu Jeong, Jaeseung Yoon, and Kwanghee Baek*

Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea, 1These authors contributed equally to this work.

Correspondence to : *Correspondence: khbaek@khu.ac.kr

Received: March 8, 2011; Revised: March 30, 2011; Accepted: March 31, 2011

Abstract

Vascular endothelial growth factor (VEGF) signaling plays an important role in angiogenesis. In the VEGF signaling pathway, the key components are VEGF and its receptors, Flt-1 and KDR. In this study, we show that transfection of synthetic miR-200b reduced protein levels of VEGF, Flt-1, and KDR. In A549 cells, miR-200b targeted the predicted binding sites in the 3?-untranslated region (3?-UTR) of VEGF, Flt-1, and KDR as revealed by a luciferase reporter assay. When transfected with miR-200b, the ability of HUVECs to form a capillary tube on Matrigel and VEGF-in-duced phosphorylation of ERK1/2 were significantly reduced. Taken together, these results suggest that miR-200b negatively regulates VEGF signaling by targeting VEGF and its receptors and that miR-200b may have therapeutic potential as an angiogenesis inhibitor.

Keywords Flt-1, KDR, microRNA, miR-200b, VEGF, VEGF signaling

Article

Research Article

Mol. Cells 2011; 32(1): 77-82

Published online July 31, 2011 https://doi.org/10.1007/s10059-011-1042-2

Copyright © The Korean Society for Molecular and Cellular Biology.

Regulation of Vascular Endothelial Growth Factor Signaling by miR-200b

Young-Chul Choi1, Sena Yoon1, Yongsu Jeong, Jaeseung Yoon, and Kwanghee Baek*

Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea, 1These authors contributed equally to this work.

Correspondence to:*Correspondence: khbaek@khu.ac.kr

Received: March 8, 2011; Revised: March 30, 2011; Accepted: March 31, 2011

Abstract

Vascular endothelial growth factor (VEGF) signaling plays an important role in angiogenesis. In the VEGF signaling pathway, the key components are VEGF and its receptors, Flt-1 and KDR. In this study, we show that transfection of synthetic miR-200b reduced protein levels of VEGF, Flt-1, and KDR. In A549 cells, miR-200b targeted the predicted binding sites in the 3?-untranslated region (3?-UTR) of VEGF, Flt-1, and KDR as revealed by a luciferase reporter assay. When transfected with miR-200b, the ability of HUVECs to form a capillary tube on Matrigel and VEGF-in-duced phosphorylation of ERK1/2 were significantly reduced. Taken together, these results suggest that miR-200b negatively regulates VEGF signaling by targeting VEGF and its receptors and that miR-200b may have therapeutic potential as an angiogenesis inhibitor.

Keywords: Flt-1, KDR, microRNA, miR-200b, VEGF, VEGF signaling

Mol. Cells
Nov 30, 2023 Vol.46 No.11, pp. 655~725
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Kim et al. (pp. 710-724) demonstrated that a pathogen-derived Ralstonia pseudosolanacearum type III effector RipL delays flowering time and enhances susceptibility to bacterial infection in Arabidopsis thaliana. Shown is the RipL-expressing Arabidopsis plant, which displays general dampening of the transcriptional program during pathogen infection, grown in long-day conditions.

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