Yusang Choi" /> Su-Jin Park" /> Yusang Choi, Ha-Young Park, Seong Joon Park, Su-Jin Park, Soo-Kyoung Kim, Changwan Ha, Su-Jin Im, and Joon-Hee Lee*" /> Yusang Choi, Ha-Young Park, Seong Joon Park, Su-Jin Park, Soo-Kyoung Kim, Changwan Ha, Su-Jin Im, and Joon-Hee Lee*. Mol. Cells 2011;32:57-65. https://doi.org/10.1007/s10059-011-2322-6">
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Mol. Cells 2011; 32(1): 57-65

Published online July 31, 2011

https://doi.org/10.1007/s10059-011-2322-6

© The Korean Society for Molecular and Cellular Biology

Growth Phase-Differential Quorum Sensing Regulation of Anthranilate Metabolism in Pseudomonas aeruginosa

Yusang Choi1,2, Ha-Young Park1,2, Seong Joon Park1, Su-Jin Park1, Soo-Kyoung Kim1, Changwan Ha1, Su-Jin Im1, and Joon-Hee Lee1,*

1Department of Pharmacy, College of Pharmacy, Pusan National University, Busan 609-735, Korea, 2These authors contributed equally to this work.

Correspondence to : *Correspondence: joonhee@pusan.ac.kr

Received: December 29, 2011; Revised: February 7, 2011; Accepted: April 25, 2011

Abstract

Pseudomonas quinolone signal (PQS) plays a role in the regulation of virulence genes and it is intertwined in the las/rhl quorum sensing (QS) circuits of Pseudomonas aeruginosa. PQS is synthesized from anthranilate by pqsA-D and pqsH whose expression is influenced by the las/rhl systems. Since anthranilate can be degraded by functions of antABC and catBCA, PQS synthesis might be regulated by the balance between the expression of the pqsA-D/phnAB, pqsH, antABC, and catBCA gene loci. antA and catA are repressed by LasR during log phase and activated by RhlR in late stationary phase, whereas pqsA-E/phnAB is activated by LasR in log phase and repressed by RhlR. QscR represses both but each repres-sion occurs in a different growth phase. This growth phase- differential regulation appears to be accomplished by the antagonistic interplay of LasR, RhlR, and QscR, mediated by two intermediate regula-tors, AntR and PqsR, and their cofactors, anthranilate and PQS, where the expressions of antR and pqsR and the production of anthranilate and PQS are growth phase-differentially regulated by QS systems. Especially, the anthranilate level increases in an RhlR-dependent manner at late stationary phase. From these results, we suggest that RhlR and LasR regulate the anthranilate metabolism in a mutually antagonistic and growth phase-differential manner by affecting both the expres-sions and activities of AntR and PqsR, and that QscR also phase-differentially represses both LasR and RhlR functions in this regulation.

Keywords anthranilate, PQS, Pseudomonas aeruginosa, QscR, quorum sensing

Article

Research Article

Mol. Cells 2011; 32(1): 57-65

Published online July 31, 2011 https://doi.org/10.1007/s10059-011-2322-6

Copyright © The Korean Society for Molecular and Cellular Biology.

Growth Phase-Differential Quorum Sensing Regulation of Anthranilate Metabolism in Pseudomonas aeruginosa

Yusang Choi1,2, Ha-Young Park1,2, Seong Joon Park1, Su-Jin Park1, Soo-Kyoung Kim1, Changwan Ha1, Su-Jin Im1, and Joon-Hee Lee1,*

1Department of Pharmacy, College of Pharmacy, Pusan National University, Busan 609-735, Korea, 2These authors contributed equally to this work.

Correspondence to:*Correspondence: joonhee@pusan.ac.kr

Received: December 29, 2011; Revised: February 7, 2011; Accepted: April 25, 2011

Abstract

Pseudomonas quinolone signal (PQS) plays a role in the regulation of virulence genes and it is intertwined in the las/rhl quorum sensing (QS) circuits of Pseudomonas aeruginosa. PQS is synthesized from anthranilate by pqsA-D and pqsH whose expression is influenced by the las/rhl systems. Since anthranilate can be degraded by functions of antABC and catBCA, PQS synthesis might be regulated by the balance between the expression of the pqsA-D/phnAB, pqsH, antABC, and catBCA gene loci. antA and catA are repressed by LasR during log phase and activated by RhlR in late stationary phase, whereas pqsA-E/phnAB is activated by LasR in log phase and repressed by RhlR. QscR represses both but each repres-sion occurs in a different growth phase. This growth phase- differential regulation appears to be accomplished by the antagonistic interplay of LasR, RhlR, and QscR, mediated by two intermediate regula-tors, AntR and PqsR, and their cofactors, anthranilate and PQS, where the expressions of antR and pqsR and the production of anthranilate and PQS are growth phase-differentially regulated by QS systems. Especially, the anthranilate level increases in an RhlR-dependent manner at late stationary phase. From these results, we suggest that RhlR and LasR regulate the anthranilate metabolism in a mutually antagonistic and growth phase-differential manner by affecting both the expres-sions and activities of AntR and PqsR, and that QscR also phase-differentially represses both LasR and RhlR functions in this regulation.

Keywords: anthranilate, PQS, Pseudomonas aeruginosa, QscR, quorum sensing

Mol. Cells
Nov 30, 2022 Vol.45 No.11, pp. 763~867
COVER PICTURE
Naive (cyan) and axotomized (magenta) retinal ganglion cell axons in Xenopus tropicalis (Choi et al., pp. 846-854).

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