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Mol. Cells 2011; 31(6): 553-561

Published online April 20, 2011

https://doi.org/10.1007/s10059-011-1038-y

© The Korean Society for Molecular and Cellular Biology

Identification and Characterization of the Du-plicate Rice Sucrose Synthase Genes OsSUS5 and OsSUS7 Which Are Associated with the Plasma Membrane

Jung-Il Cho, Hyun-Bi Kim, Chi-Yeol Kim, Tae-Ryong Hahn, and Jong-Seong Jeon*

Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea

Correspondence to : *Correspondence: jjeon@khu.ac.kr

Received: March 4, 2011; Revised: March 21, 2011; Accepted: March 22, 2011

Abstract

Systematic searches using the complete genome se-quence of rice (Oryza sativa) identified OsSUS7, a new member of the rice sucrose synthase (OsSUS) gene family, which shows only nine single nucleotide substitutions in the OsSUS5 coding sequence. Comparative genomic analysis revealed that the synteny between OsSUS5 and OsSUS7 is conserved, and that significant numbers of transposable elements are scattered at both loci. In particular, a 17.6-kb genomic region containing transposable elements was identified in the 5' upstream sequence of the OsSUS7 gene. GFP fusion experiments indicated that OsSUS5 and OsSUS7 are largely associated with the plasma membrane and partly with the cytosol in maize mesophyll protoplasts. RT-PCR analysis and transient expression assays revealed that OsSUS5 and OsSUS7 exhibit similar expression patterns in rice tissues, with the highest ex-pression evident in roots. These results suggest that two redundant genes, OsSUS5 and OsSUS7, evolved via dup-lication of a chromosome region and through the transposition of transposable elements.

Keywords duplication, localization, rice, sucrose synthase, transposable element

Article

Research Article

Mol. Cells 2011; 31(6): 553-561

Published online June 30, 2011 https://doi.org/10.1007/s10059-011-1038-y

Copyright © The Korean Society for Molecular and Cellular Biology.

Identification and Characterization of the Du-plicate Rice Sucrose Synthase Genes OsSUS5 and OsSUS7 Which Are Associated with the Plasma Membrane

Jung-Il Cho, Hyun-Bi Kim, Chi-Yeol Kim, Tae-Ryong Hahn, and Jong-Seong Jeon*

Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea

Correspondence to:*Correspondence: jjeon@khu.ac.kr

Received: March 4, 2011; Revised: March 21, 2011; Accepted: March 22, 2011

Abstract

Systematic searches using the complete genome se-quence of rice (Oryza sativa) identified OsSUS7, a new member of the rice sucrose synthase (OsSUS) gene family, which shows only nine single nucleotide substitutions in the OsSUS5 coding sequence. Comparative genomic analysis revealed that the synteny between OsSUS5 and OsSUS7 is conserved, and that significant numbers of transposable elements are scattered at both loci. In particular, a 17.6-kb genomic region containing transposable elements was identified in the 5' upstream sequence of the OsSUS7 gene. GFP fusion experiments indicated that OsSUS5 and OsSUS7 are largely associated with the plasma membrane and partly with the cytosol in maize mesophyll protoplasts. RT-PCR analysis and transient expression assays revealed that OsSUS5 and OsSUS7 exhibit similar expression patterns in rice tissues, with the highest ex-pression evident in roots. These results suggest that two redundant genes, OsSUS5 and OsSUS7, evolved via dup-lication of a chromosome region and through the transposition of transposable elements.

Keywords: duplication, localization, rice, sucrose synthase, transposable element

Mol. Cells
Mar 31, 2023 Vol.46 No.3, pp. 131~189
COVER PICTURE
The physiologically important cytoprotective signaling in normal cells (background area in turquoise) mediated by NRF2 (blue chain) is often hijacked by cancer cells (red ball) in the tumor microenvironment (yellow area). However, the differential roles of NRF2 throughout the multistage carcinogenesis remains largely unresolved (white-colored overlapping misty areas).

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