Min-Jung Han " /> " /> Gihwan Yi " /> Min-Jung Han, Ki-Hong Jung, Gihwan Yi, and Gynheung An*" /> Min-Jung Han, Ki-Hong Jung, Gihwan Yi, and Gynheung An*. Mol. Cells 2011;31:523-30. https://doi.org/10.1007/s10059-011-2321-7">
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Mol. Cells 2011; 31(6): 523-530

Published online June 30, 2011

https://doi.org/10.1007/s10059-011-2321-7

© The Korean Society for Molecular and Cellular Biology

Rice Importin ß1 Gene Affects Pollen Tube Elongation

Min-Jung Han1,3, Ki-Hong Jung1, Gihwan Yi2, and Gynheung An1,*

1Crop Biotech Institute and Department of Plant Molecular Systems Biotechnology, Kyung Hee University, Yongin 446-701, Korea, 2International Technical Cooperation Center, Rural Development Administration, Suwon 441-707, Korea, 3Present address: POSTECH Biotechnology Center, Pohang University of Science and Technology, Pohang 790-784, Korea

Correspondence to : *Correspondence: genean@khu.ac.kr

Received: December 29, 2011; Revised: March 17, 2011; Accepted: March 18, 2011

Abstract

Importin ß1 interacts with nuclear transport factors and mediates the import of nuclear proteins. We isolated a pollen-expressed gene, rice Importin ß1 (OsImp ß1), from a T-DNA insertional population that was trapped by a promoterless ß-glucuronidase (GUS) gene. The GUS reporter was expressed in the anthers and ovaries from early through mature developmental stages. Its expression was also observed in all floral organs. However, these patterns changed as the spikelet developed. T-DNA was inserted into the OsImp ß1 gene at 339 bp downstream from the translation initiation site. We obtained another T-DNA insertional allele by searching the flanking sequence tag database. In both lines, the wild-type and T-DNA-carrying progeny segregated at a ratio close to 1:1. The latter genotype was heterozygous (OsImp ß1/osimp ß1). Reciprocal crosses between WT and heterozygous plants demon-strated that the mutant alleles could not be transmitted through the male gametophyte. Close examination of the heterozygous anthers revealed that the mutant pollen matured normally. However, in vitro assays showed that tube elongation was hampered in the mutant grains. These results indicate that OsImp ß1 is specifically required for pollen tube elongation.

Keywords GUS, importin

Article

Research Article

Mol. Cells 2011; 31(6): 523-530

Published online June 30, 2011 https://doi.org/10.1007/s10059-011-2321-7

Copyright © The Korean Society for Molecular and Cellular Biology.

Rice Importin ß1 Gene Affects Pollen Tube Elongation

Min-Jung Han1,3, Ki-Hong Jung1, Gihwan Yi2, and Gynheung An1,*

1Crop Biotech Institute and Department of Plant Molecular Systems Biotechnology, Kyung Hee University, Yongin 446-701, Korea, 2International Technical Cooperation Center, Rural Development Administration, Suwon 441-707, Korea, 3Present address: POSTECH Biotechnology Center, Pohang University of Science and Technology, Pohang 790-784, Korea

Correspondence to:*Correspondence: genean@khu.ac.kr

Received: December 29, 2011; Revised: March 17, 2011; Accepted: March 18, 2011

Abstract

Importin ß1 interacts with nuclear transport factors and mediates the import of nuclear proteins. We isolated a pollen-expressed gene, rice Importin ß1 (OsImp ß1), from a T-DNA insertional population that was trapped by a promoterless ß-glucuronidase (GUS) gene. The GUS reporter was expressed in the anthers and ovaries from early through mature developmental stages. Its expression was also observed in all floral organs. However, these patterns changed as the spikelet developed. T-DNA was inserted into the OsImp ß1 gene at 339 bp downstream from the translation initiation site. We obtained another T-DNA insertional allele by searching the flanking sequence tag database. In both lines, the wild-type and T-DNA-carrying progeny segregated at a ratio close to 1:1. The latter genotype was heterozygous (OsImp ß1/osimp ß1). Reciprocal crosses between WT and heterozygous plants demon-strated that the mutant alleles could not be transmitted through the male gametophyte. Close examination of the heterozygous anthers revealed that the mutant pollen matured normally. However, in vitro assays showed that tube elongation was hampered in the mutant grains. These results indicate that OsImp ß1 is specifically required for pollen tube elongation.

Keywords: GUS, importin

Mol. Cells
Sep 30, 2022 Vol.45 No.9, pp. 603~672
COVER PICTURE
The Target of Rapamycin Complex (TORC) is a central regulatory hub in eukaryotes, which is well conserved in diverse plant species, including tomato (Solanum lycopersicum). Inhibition of TORC genes (SlTOR, SlLST8, and SlRAPTOR) by VIGS (virus-induced gene silencing) results in early fruit ripening in tomato. The red/ orange tomatoes are early-ripened TORC-silenced fruits, while the green tomato is a control fruit. Top, left, control fruit (TRV2-myc); top, right, TRV2-SlLST8; bottom, left, TRV2-SlTOR; bottom, right, TRV2-SlRAPTOR(Choi et al., pp. 660-672).

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