Krzysztof Szade" /> Krzysztof Szade, Ewa Zuba-Surma, Andrzej J. Rutkowski, Alicja Jozkowicz, and Jozef Dulak*" /> Krzysztof Szade, Ewa Zuba-Surma, Andrzej J. Rutkowski, Alicja Jozkowicz, and Jozef Dulak*. Mol. Cells 2011;31:497-507. https://doi.org/10.1007/s10059-011-2176-y">
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Mol. Cells 2011; 31(6): 497-507

Published online April 22, 2011

https://doi.org/10.1007/s10059-011-2176-y

© The Korean Society for Molecular and Cellular Biology

CD45-CD14+CD34+Murine Bone Marrow Low-Adherent Mesenchymal Primitive Cells Preserve Multilineage Differentiation Potential in Long-Term In Vitro Culture

Krzysztof Szade1, Ewa Zuba-Surma1, Andrzej J. Rutkowski1,2, Alicja Jozkowicz1, and Jozef Dulak1,*

1Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland, 2Present address: The Paterson Institute for Cancer Research, The University of Manchester, United Kingdom

Correspondence to : *Correspondence: jozef.dulak@uj.edu.pl

Received: July 13, 2011; Revised: March 7, 2011; Accepted: March 29, 2011

Abstract

Bone marrow-derived cells have been postulated as a source of multipotent mesenchymal stem cells (MSC). However, the whole fraction of MSC remains heterogene-ous and the expansion of primitive subset of these cells is still not well established. Here, we optimized the protocol for propagating the low-adherent subfraction of MSC which results in long-term expansion of population characterized by CD45-CD14+CD34+ phenotype along with expression of common MSC markers. We established that the expanded MSC are capable of differentiating into endothelial cells highly expressing angiogenic markers and exhibiting functional properties of endothelium. Moreover, we found these cells to be multipotent and capable of giving rise into cells from neuronal lineages. Interestingly, the expanded MSC form characteristic cellular spheres in vitro indicating primitive features of these cells. In sum, we isolated the novel multipotent subpopulation of CD45-CD14+CD34+ bone marrow-derived cells that could be maintained in long-term culture without losing this potential.

Keywords bone marrow, differentiation, endothelium, expansion, mesenchymal stem cells

Article

Research Article

Mol. Cells 2011; 31(6): 497-507

Published online June 30, 2011 https://doi.org/10.1007/s10059-011-2176-y

Copyright © The Korean Society for Molecular and Cellular Biology.

CD45-CD14+CD34+Murine Bone Marrow Low-Adherent Mesenchymal Primitive Cells Preserve Multilineage Differentiation Potential in Long-Term In Vitro Culture

Krzysztof Szade1, Ewa Zuba-Surma1, Andrzej J. Rutkowski1,2, Alicja Jozkowicz1, and Jozef Dulak1,*

1Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland, 2Present address: The Paterson Institute for Cancer Research, The University of Manchester, United Kingdom

Correspondence to:*Correspondence: jozef.dulak@uj.edu.pl

Received: July 13, 2011; Revised: March 7, 2011; Accepted: March 29, 2011

Abstract

Bone marrow-derived cells have been postulated as a source of multipotent mesenchymal stem cells (MSC). However, the whole fraction of MSC remains heterogene-ous and the expansion of primitive subset of these cells is still not well established. Here, we optimized the protocol for propagating the low-adherent subfraction of MSC which results in long-term expansion of population characterized by CD45-CD14+CD34+ phenotype along with expression of common MSC markers. We established that the expanded MSC are capable of differentiating into endothelial cells highly expressing angiogenic markers and exhibiting functional properties of endothelium. Moreover, we found these cells to be multipotent and capable of giving rise into cells from neuronal lineages. Interestingly, the expanded MSC form characteristic cellular spheres in vitro indicating primitive features of these cells. In sum, we isolated the novel multipotent subpopulation of CD45-CD14+CD34+ bone marrow-derived cells that could be maintained in long-term culture without losing this potential.

Keywords: bone marrow, differentiation, endothelium, expansion, mesenchymal stem cells

Mol. Cells
May 31, 2023 Vol.46 No.5, pp. 259~328
COVER PICTURE
The alpha-helices in the lamin filaments are depicted as coils, with different subdomains distinguished by various colors. Coil 1a is represented by magenta, coil 1b by yellow, L2 by green, coil 2a by white, coil 2b by brown, stutter by cyan, coil 2c by dark blue, and the lamin Ig-like domain by grey. In the background, cells are displayed, with the cytosol depicted in green and the nucleus in blue (Ahn et al., pp. 309-318).

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