Mol. Cells 2011; 31(5): 477-481
Published online May 31, 2011
https://doi.org/10.1007/s10059-011-1035-1
© The Korean Society for Molecular and Cellular Biology
Meenakshi Dwivedi1,6, Hyun Sung2,6, Haihong Shen3, Byung-Jae Park4,*, and Sangho Lee5,*
Correspondence to : *Correspondence: bjparkkk@hallym.ac.kr (BJP); sangholee@skku.edu (SL)
Autophagy and endocytic pathway are highly regulated catabolic processes. Both processes are crucial for cell growth, development, differentiation, disease and homeostasis and exhibit membrane rearrangement for their function. Autophagy and endocytic pathway represent branches of the lysosomal digestive system, autophagy being responsible for degradation of cyto-plasmic components and endocytic pathway for degradation of exogenous substances. Here we report that autophagy is activated when endocytic pathway regulatory genes such as rab-5 and rabx-5 are disrupted. Defects in the ubiquitin binding domain of RABX-5 are critical in activating autophagy. We also observed that the elevated autophagy level does not contribute to lifespan extension of rabx-5 mutant. Our results suggest that autophagy may compensate for the endocytic pathway when regulatory genes for the endocytic pathway malfunction, providing a case of complementa-tion between two functionally related cellular processes.
Keywords autophagy, endocytic pathway, GFP::LGG-1, RAB-5, RABX-5
Mol. Cells 2011; 31(5): 477-481
Published online May 31, 2011 https://doi.org/10.1007/s10059-011-1035-1
Copyright © The Korean Society for Molecular and Cellular Biology.
Meenakshi Dwivedi1,6, Hyun Sung2,6, Haihong Shen3, Byung-Jae Park4,*, and Sangho Lee5,*
1Dr. B.R. Ambedkar Center for Biomedical Research, University of Delhi, Delhi, India, 2Department of Biological Sciences, Purdue University, USA, 3School of Life Science, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea, 4Department of Life Science, Hallym University, Chunchon 200-702, Korea, 5Department of Biological Sciences, Sungkyunkwan University, Suwon 440-746, Korea, 6These authors contributed equally to this work.
Correspondence to:*Correspondence: bjparkkk@hallym.ac.kr (BJP); sangholee@skku.edu (SL)
Autophagy and endocytic pathway are highly regulated catabolic processes. Both processes are crucial for cell growth, development, differentiation, disease and homeostasis and exhibit membrane rearrangement for their function. Autophagy and endocytic pathway represent branches of the lysosomal digestive system, autophagy being responsible for degradation of cyto-plasmic components and endocytic pathway for degradation of exogenous substances. Here we report that autophagy is activated when endocytic pathway regulatory genes such as rab-5 and rabx-5 are disrupted. Defects in the ubiquitin binding domain of RABX-5 are critical in activating autophagy. We also observed that the elevated autophagy level does not contribute to lifespan extension of rabx-5 mutant. Our results suggest that autophagy may compensate for the endocytic pathway when regulatory genes for the endocytic pathway malfunction, providing a case of complementa-tion between two functionally related cellular processes.
Keywords: autophagy, endocytic pathway, GFP::LGG-1, RAB-5, RABX-5
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