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Mol. Cells 2011; 31(5): 447-454

Published online May 31, 2011

https://doi.org/10.1007/s10059-011-0300-7

© The Korean Society for Molecular and Cellular Biology

Overexpression of AtMYB52 Confers ABA Hypersensitivity and Drought Tolerance

Min Young Park, Jung-youn Kang, and Soo Young Kim*

Department of Molecular Biotechnology and Kumho Life Science Laboratory, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Korea

Correspondence to : *Correspondence: sooykim@chonnam.ac.kr

Received: December 3, 2011; Revised: January 22, 2011; Accepted: February 7, 2011

Abstract

We carried out activation tagging screen to isolate genes regulating abscisic acid (ABA) response. From the screen of approximately 10,000 plants, we isolated ca 100 ABA response mutants. We characterized one of the mutants, designated ahs1, in this study. The mutant is ABA-hypersensitive, and AtMYB52 was found to be activated in the mutant. Overexpression analysis to recapitulate the mutant phenotypes demonstrated that ATMYB confers ABA-hypersensitivity during postgermination growth. Additionally, AtMYB52 overexpression lines were drought-tolerant and their seedlings were salt-sensitive. Changes in the expression levels of a few genes involved in ABA response or cell wall biosynthesis were also observed. Together, our data suggest that AtMYB52 is involved in ABA response. Others previously demonstrated that AtMYB52 regulates cell wall biosynthesis; thus, our results imply a possible connec-tion between ABA response and cell wall biosynthesis.

Keywords abscisic acid (ABA), activation tagging, cell wall, MYB protein, stress response

Article

Research Article

Mol. Cells 2011; 31(5): 447-454

Published online May 31, 2011 https://doi.org/10.1007/s10059-011-0300-7

Copyright © The Korean Society for Molecular and Cellular Biology.

Overexpression of AtMYB52 Confers ABA Hypersensitivity and Drought Tolerance

Min Young Park, Jung-youn Kang, and Soo Young Kim*

Department of Molecular Biotechnology and Kumho Life Science Laboratory, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Korea

Correspondence to:*Correspondence: sooykim@chonnam.ac.kr

Received: December 3, 2011; Revised: January 22, 2011; Accepted: February 7, 2011

Abstract

We carried out activation tagging screen to isolate genes regulating abscisic acid (ABA) response. From the screen of approximately 10,000 plants, we isolated ca 100 ABA response mutants. We characterized one of the mutants, designated ahs1, in this study. The mutant is ABA-hypersensitive, and AtMYB52 was found to be activated in the mutant. Overexpression analysis to recapitulate the mutant phenotypes demonstrated that ATMYB confers ABA-hypersensitivity during postgermination growth. Additionally, AtMYB52 overexpression lines were drought-tolerant and their seedlings were salt-sensitive. Changes in the expression levels of a few genes involved in ABA response or cell wall biosynthesis were also observed. Together, our data suggest that AtMYB52 is involved in ABA response. Others previously demonstrated that AtMYB52 regulates cell wall biosynthesis; thus, our results imply a possible connec-tion between ABA response and cell wall biosynthesis.

Keywords: abscisic acid (ABA), activation tagging, cell wall, MYB protein, stress response

Mol. Cells
May 31, 2022 Vol.45 No.5, pp. 273~352
COVER PICTURE
Fe2+ ion depletion-induced expression of BΔGFP at the early stage of leaf development (Choi et al., pp. 294-305).

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