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Mol. Cells 2011; 31(5): 399-403

Published online February 25, 2011

https://doi.org/10.1007/s10059-011-1010-x

© The Korean Society for Molecular and Cellular Biology

The Role of Sphingosine 1-Phosphate in Migration of Osteoclast Precursors; an Application of Intravital Two-Photon Microscopy

Taeko Ishii, Yutaka Shimazu, Issei Nishiyama, Junichi Kikuta, and Masaru Ishii*

Laboratory of Biological Imaging, WPI-Immunology Frontier Research Center, Osaka University, Osaka, Japan

Correspondence to : *Correspondence: mishii@ifrec.osaka-u.ac.jp

Received: January 13, 2011; Accepted: January 31, 2011

Abstract

Sphingosine-1-phosphate (S1P), a biologically active lysophospholipid that is enriched in blood, controls the trafficking of osteoclast precursors between the circula-tion and bone marrow cavities via G protein-coupled receptors, S1PRs. While S1PR1 mediates chemoattraction toward S1P in bone marrow, where S1P concentration is low, S1PR2 mediates chemorepulsion in blood, where the S1P concentration is high. The regulation of precursor recruitment may represent a novel therapeutic strategy for controlling osteoclast-dependent bone remodeling. Through intravital multiphoton imaging of bone tissues, we reveal that the bidirectional function of S1P temporospatially regulates the migration of osteoclast precursors within intact bone tissues. Imaging technologies have enabled in situ visualization of the behaviors of several players in intact tissues. In addition, intravital microscopy has the potential to be more widely applied to functional analysis and intervention.

Keywords cell dynamics, chemokine, chemotaxis, lipid mediator, live imaging

Article

Minireview

Mol. Cells 2011; 31(5): 399-403

Published online May 31, 2011 https://doi.org/10.1007/s10059-011-1010-x

Copyright © The Korean Society for Molecular and Cellular Biology.

The Role of Sphingosine 1-Phosphate in Migration of Osteoclast Precursors; an Application of Intravital Two-Photon Microscopy

Taeko Ishii, Yutaka Shimazu, Issei Nishiyama, Junichi Kikuta, and Masaru Ishii*

Laboratory of Biological Imaging, WPI-Immunology Frontier Research Center, Osaka University, Osaka, Japan

Correspondence to:*Correspondence: mishii@ifrec.osaka-u.ac.jp

Received: January 13, 2011; Accepted: January 31, 2011

Abstract

Sphingosine-1-phosphate (S1P), a biologically active lysophospholipid that is enriched in blood, controls the trafficking of osteoclast precursors between the circula-tion and bone marrow cavities via G protein-coupled receptors, S1PRs. While S1PR1 mediates chemoattraction toward S1P in bone marrow, where S1P concentration is low, S1PR2 mediates chemorepulsion in blood, where the S1P concentration is high. The regulation of precursor recruitment may represent a novel therapeutic strategy for controlling osteoclast-dependent bone remodeling. Through intravital multiphoton imaging of bone tissues, we reveal that the bidirectional function of S1P temporospatially regulates the migration of osteoclast precursors within intact bone tissues. Imaging technologies have enabled in situ visualization of the behaviors of several players in intact tissues. In addition, intravital microscopy has the potential to be more widely applied to functional analysis and intervention.

Keywords: cell dynamics, chemokine, chemotaxis, lipid mediator, live imaging

Mol. Cells
May 31, 2023 Vol.46 No.5, pp. 259~328
COVER PICTURE
The alpha-helices in the lamin filaments are depicted as coils, with different subdomains distinguished by various colors. Coil 1a is represented by magenta, coil 1b by yellow, L2 by green, coil 2a by white, coil 2b by brown, stutter by cyan, coil 2c by dark blue, and the lamin Ig-like domain by grey. In the background, cells are displayed, with the cytosol depicted in green and the nucleus in blue (Ahn et al., pp. 309-318).

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