Mol. Cells 2011; 31(5): 399-403
Published online February 25, 2011
https://doi.org/10.1007/s10059-011-1010-x
© The Korean Society for Molecular and Cellular Biology
Correspondence to : *Correspondence: mishii@ifrec.osaka-u.ac.jp
Sphingosine-1-phosphate (S1P), a biologically active lysophospholipid that is enriched in blood, controls the trafficking of osteoclast precursors between the circula-tion and bone marrow cavities via G protein-coupled receptors, S1PRs. While S1PR1 mediates chemoattraction toward S1P in bone marrow, where S1P concentration is low, S1PR2 mediates chemorepulsion in blood, where the S1P concentration is high. The regulation of precursor recruitment may represent a novel therapeutic strategy for controlling osteoclast-dependent bone remodeling. Through intravital multiphoton imaging of bone tissues, we reveal that the bidirectional function of S1P temporospatially regulates the migration of osteoclast precursors within intact bone tissues. Imaging technologies have enabled in situ visualization of the behaviors of several players in intact tissues. In addition, intravital microscopy has the potential to be more widely applied to functional analysis and intervention.
Keywords cell dynamics, chemokine, chemotaxis, lipid mediator, live imaging
Mol. Cells 2011; 31(5): 399-403
Published online May 31, 2011 https://doi.org/10.1007/s10059-011-1010-x
Copyright © The Korean Society for Molecular and Cellular Biology.
Taeko Ishii, Yutaka Shimazu, Issei Nishiyama, Junichi Kikuta, and Masaru Ishii*
Laboratory of Biological Imaging, WPI-Immunology Frontier Research Center, Osaka University, Osaka, Japan
Correspondence to:*Correspondence: mishii@ifrec.osaka-u.ac.jp
Sphingosine-1-phosphate (S1P), a biologically active lysophospholipid that is enriched in blood, controls the trafficking of osteoclast precursors between the circula-tion and bone marrow cavities via G protein-coupled receptors, S1PRs. While S1PR1 mediates chemoattraction toward S1P in bone marrow, where S1P concentration is low, S1PR2 mediates chemorepulsion in blood, where the S1P concentration is high. The regulation of precursor recruitment may represent a novel therapeutic strategy for controlling osteoclast-dependent bone remodeling. Through intravital multiphoton imaging of bone tissues, we reveal that the bidirectional function of S1P temporospatially regulates the migration of osteoclast precursors within intact bone tissues. Imaging technologies have enabled in situ visualization of the behaviors of several players in intact tissues. In addition, intravital microscopy has the potential to be more widely applied to functional analysis and intervention.
Keywords: cell dynamics, chemokine, chemotaxis, lipid mediator, live imaging
Taeko Ishii, Yutaka Shimazu, Issei Nishiyama, Junichi Kikuta, and Masaru Ishii*
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