Mol. Cells 2011; 31(4): 393-397
Published online February 22, 2011
https://doi.org/10.1007/s10059-011-0046-2
© The Korean Society for Molecular and Cellular Biology
Changfa Lin1, Hee-Seung Choi1, and Hyung-Taeg Cho*
Correspondence to : *Correspondence: htcho@snu.ac.kr
Expansins are non-hydrolytic cell wall-loosening pro-teins that are involved in the cell wall modifications that underlie many plant developmental processes. Root hair growth requires the accumulation of cell wall materials and dynamic cell wall modification at the tip region. Al-though several lines of indirect evidence support the idea that expansin-mediated wall modification occurs during root hair growth, the involvement of these proteins remains to be demonstrated in vivo. In this study, we used RNA interference (RNAi) to examine the biological function of Arabidopsis thaliana EXPANSIN A7 (AtEXPA7), which is expressed specifically in the root hair cell. The root hair-specific AtEXPA7 promoter was used to drive RNAi expression, which targeted two independent regions in the AtEXPA7 transcript. Quantitative reverse transcriptase-PCR analyses were used to examine AtEXPA7 transcript levels. In four independent RNAi transformant lines, RNAi expression reduced AtEXPA7 transcript levels by 25-58% compared to controls. Accordingly, the root hairs of RNAi transformant lines were 25-48% shorter than control plants and exhibited a broader range of lengths than the controls. Our results provide in vivo evidence that ex-pansins are required for root hair tip growth.
Keywords Arabidopsis, cell wall, expansin, root hair
Mol. Cells 2011; 31(4): 393-397
Published online April 30, 2011 https://doi.org/10.1007/s10059-011-0046-2
Copyright © The Korean Society for Molecular and Cellular Biology.
Changfa Lin1, Hee-Seung Choi1, and Hyung-Taeg Cho*
Department of Biological Sciences and Genomics and Breeding Institute, Seoul National University, Seoul 151-742, Korea, 1These authors contributed equally to this work.
Correspondence to:*Correspondence: htcho@snu.ac.kr
Expansins are non-hydrolytic cell wall-loosening pro-teins that are involved in the cell wall modifications that underlie many plant developmental processes. Root hair growth requires the accumulation of cell wall materials and dynamic cell wall modification at the tip region. Al-though several lines of indirect evidence support the idea that expansin-mediated wall modification occurs during root hair growth, the involvement of these proteins remains to be demonstrated in vivo. In this study, we used RNA interference (RNAi) to examine the biological function of Arabidopsis thaliana EXPANSIN A7 (AtEXPA7), which is expressed specifically in the root hair cell. The root hair-specific AtEXPA7 promoter was used to drive RNAi expression, which targeted two independent regions in the AtEXPA7 transcript. Quantitative reverse transcriptase-PCR analyses were used to examine AtEXPA7 transcript levels. In four independent RNAi transformant lines, RNAi expression reduced AtEXPA7 transcript levels by 25-58% compared to controls. Accordingly, the root hairs of RNAi transformant lines were 25-48% shorter than control plants and exhibited a broader range of lengths than the controls. Our results provide in vivo evidence that ex-pansins are required for root hair tip growth.
Keywords: Arabidopsis, cell wall, expansin, root hair
Su-Kyung Won, Sang-Bong Choi, Simple Kumari, Misuk Cho, Sang Ho Lee, and Hyung-Taeg Cho*
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