Jin-Ah Park" /> Ae-Jin Kim" /> Yoonsung Kang" /> Jin-Ah Park, Ae-Jin Kim, Yoonsung Kang, Yu-Jin Jung, Hyong Kyu Kim, and Keun-Cheol Kim*" /> Jin-Ah Park, Ae-Jin Kim, Yoonsung Kang, Yu-Jin Jung, Hyong Kyu Kim, and Keun-Cheol Kim*. Mol. Cells 2011;31:343-9. https://doi.org/10.1007/s10059-011-0044-4">
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Mol. Cells 2011; 31(4): 343-349

Published online February 22, 2011

https://doi.org/10.1007/s10059-011-0044-4

© The Korean Society for Molecular and Cellular Biology

Deacetylation and Methylation at Histone H3 Lysine 9 (H3K9) Coordinate Chromosome Condensation during Cell Cycle Progression

Jin-Ah Park1,4, Ae-Jin Kim1,4, Yoonsung Kang2, Yu-Jin Jung1, Hyong Kyu Kim3, and Keun-Cheol Kim1,*

1Medical and Bio-Material Research Center and Department of Biology, College of Natural Sciences, Kangwon National University, Chuncheon 200-701, Korea, 2DNA Repair Research Center and Department of Bio-Materials, Chosun University, Gwangju 501-759, Korea, 3College of Medicine, Chungbuk National University, Cheongju 361-763, Korea, 4These authors contributed equally to this work.

Correspondence to : *Correspondence: kckim@kangwon.ac.kr

Received: November 2, 2011; Revised: January 4, 2011; Accepted: January 13, 2011

Abstract

Interphasic chromatin condenses into the chromosomes in order to facilitate the correct segregation of genetic information. It has been previously reported that the phosphorylation and methylation of the N-terminal tail of histone H3 are responsible for chromosome condensation. In this study, we demonstrate that the deacetylation and methylation of histone H3 lysine 9 (H3K9) are required for proper chromosome condensation. We confirmed that H3K9ac levels were reduced, whereas H3K9me3 levels were increased in mitotic cells, via immunofluorescence and Western blot analysis. Nocodazole treatment induced G2/M arrest but co-treatment with TSA, an HDAC inhibitor, delayed cell cycle progression. However, the HMTase inhibitor, AdoX, had no effect on nocodazole-induced G2/M arrest, thereby indicating that sequential modifications of H3K9 are required for proper chromosome condensation. The expression of SUV39H1 and SETDB1, H3K9me3-responsible HMTases, are specifically increased along with H3K9me3 in nocodazole-arrested buoyant cells, which suggests that the increased expression of those proteins is an important step in chromosome condensa-tion. H3K9me3 was highly concentrated in the vertical chromosomal axis during prophase and prometaphase. Collectively, the results of this study indicate that sequential modifications at H3K9 are associated with correct chromosome condensation, and that H3K9me3 may be relevant to the condensation of chromosome length.

Keywords chromosome condensation, deacetylation, H3K9, methylation, SETDB1, SUV39H1

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Research Article

Mol. Cells 2011; 31(4): 343-349

Published online April 30, 2011 https://doi.org/10.1007/s10059-011-0044-4

Copyright © The Korean Society for Molecular and Cellular Biology.

Deacetylation and Methylation at Histone H3 Lysine 9 (H3K9) Coordinate Chromosome Condensation during Cell Cycle Progression

Jin-Ah Park1,4, Ae-Jin Kim1,4, Yoonsung Kang2, Yu-Jin Jung1, Hyong Kyu Kim3, and Keun-Cheol Kim1,*

1Medical and Bio-Material Research Center and Department of Biology, College of Natural Sciences, Kangwon National University, Chuncheon 200-701, Korea, 2DNA Repair Research Center and Department of Bio-Materials, Chosun University, Gwangju 501-759, Korea, 3College of Medicine, Chungbuk National University, Cheongju 361-763, Korea, 4These authors contributed equally to this work.

Correspondence to:*Correspondence: kckim@kangwon.ac.kr

Received: November 2, 2011; Revised: January 4, 2011; Accepted: January 13, 2011

Abstract

Interphasic chromatin condenses into the chromosomes in order to facilitate the correct segregation of genetic information. It has been previously reported that the phosphorylation and methylation of the N-terminal tail of histone H3 are responsible for chromosome condensation. In this study, we demonstrate that the deacetylation and methylation of histone H3 lysine 9 (H3K9) are required for proper chromosome condensation. We confirmed that H3K9ac levels were reduced, whereas H3K9me3 levels were increased in mitotic cells, via immunofluorescence and Western blot analysis. Nocodazole treatment induced G2/M arrest but co-treatment with TSA, an HDAC inhibitor, delayed cell cycle progression. However, the HMTase inhibitor, AdoX, had no effect on nocodazole-induced G2/M arrest, thereby indicating that sequential modifications of H3K9 are required for proper chromosome condensation. The expression of SUV39H1 and SETDB1, H3K9me3-responsible HMTases, are specifically increased along with H3K9me3 in nocodazole-arrested buoyant cells, which suggests that the increased expression of those proteins is an important step in chromosome condensa-tion. H3K9me3 was highly concentrated in the vertical chromosomal axis during prophase and prometaphase. Collectively, the results of this study indicate that sequential modifications at H3K9 are associated with correct chromosome condensation, and that H3K9me3 may be relevant to the condensation of chromosome length.

Keywords: chromosome condensation, deacetylation, H3K9, methylation, SETDB1, SUV39H1

Mol. Cells
Nov 30, 2023 Vol.46 No.11, pp. 655~725
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Kim et al. (pp. 710-724) demonstrated that a pathogen-derived Ralstonia pseudosolanacearum type III effector RipL delays flowering time and enhances susceptibility to bacterial infection in Arabidopsis thaliana. Shown is the RipL-expressing Arabidopsis plant, which displays general dampening of the transcriptional program during pathogen infection, grown in long-day conditions.

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