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Mol. Cells 2011; 31(4): 327-335

Published online April 30, 2011

https://doi.org/10.1007/s10059-011-0041-7

© The Korean Society for Molecular and Cellular Biology

Wogonin Induces Apoptosis by Activation of ERK and p38 MAPKs Signaling Pathways and Generation of Reactive Oxygen Species in Human Breast Cancer Cells

Ji Sun Yu, and An Keun Kim*

Biochemistry Laboratory, College of Pharmacy, Sookmyung Women’s University, Seoul 140-742, Korea

Correspondence to : *Correspondence: akkim@sookmyung.ac.kr

Received: September 27, 2011; Revised: January 3, 2011; Accepted: January 4, 2011

Abstract

Wogonin is a one of the bioactive compounds of Scutellaria baicalensi Georgi which has been shown to have antiinflammatory, anticancer, antiviral and neuroprotective effects. However, the underlying mechanisms by which wogonin induces apoptosis in cancer cells still remain speculative. Here we investigated the potential activation of MAPKs and generation of reactive oxygen species (ROS) by wogonin on MCF-7 human breast cancer cells. These results showed that wogonin induced mitochondria and death-receptor-mediated apoptotic cell death, which was characterized by activation of several caspases, induction of PARP cleavage, change of antiapoptotic/pro-apoptotic Bcl-2 family member ratios and cleavage of Bid. We also found that generation of ROS was an important mediator in wogonin-induced apoptosis. Further investigation re-vealed that wogonin activated ERK and p38 MAPKs, which was inhibited by N-acetyl cysteine (NAC), a ROS scavenger, indicating that wogonin-induced ROS are associated with MAPKs activation. These data demonstrate that wogonin may be a novel anticancer agent for treatment of breast cancer.

Keywords apoptosis, breast cancer, mitogen-activated protein kinases, reactive oxygen species, wogonin

Article

Research Article

Mol. Cells 2011; 31(4): 327-335

Published online April 30, 2011 https://doi.org/10.1007/s10059-011-0041-7

Copyright © The Korean Society for Molecular and Cellular Biology.

Wogonin Induces Apoptosis by Activation of ERK and p38 MAPKs Signaling Pathways and Generation of Reactive Oxygen Species in Human Breast Cancer Cells

Ji Sun Yu, and An Keun Kim*

Biochemistry Laboratory, College of Pharmacy, Sookmyung Women’s University, Seoul 140-742, Korea

Correspondence to:*Correspondence: akkim@sookmyung.ac.kr

Received: September 27, 2011; Revised: January 3, 2011; Accepted: January 4, 2011

Abstract

Wogonin is a one of the bioactive compounds of Scutellaria baicalensi Georgi which has been shown to have antiinflammatory, anticancer, antiviral and neuroprotective effects. However, the underlying mechanisms by which wogonin induces apoptosis in cancer cells still remain speculative. Here we investigated the potential activation of MAPKs and generation of reactive oxygen species (ROS) by wogonin on MCF-7 human breast cancer cells. These results showed that wogonin induced mitochondria and death-receptor-mediated apoptotic cell death, which was characterized by activation of several caspases, induction of PARP cleavage, change of antiapoptotic/pro-apoptotic Bcl-2 family member ratios and cleavage of Bid. We also found that generation of ROS was an important mediator in wogonin-induced apoptosis. Further investigation re-vealed that wogonin activated ERK and p38 MAPKs, which was inhibited by N-acetyl cysteine (NAC), a ROS scavenger, indicating that wogonin-induced ROS are associated with MAPKs activation. These data demonstrate that wogonin may be a novel anticancer agent for treatment of breast cancer.

Keywords: apoptosis, breast cancer, mitogen-activated protein kinases, reactive oxygen species, wogonin

Mol. Cells
Nov 30, 2022 Vol.45 No.11, pp. 763~867
COVER PICTURE
Naive (cyan) and axotomized (magenta) retinal ganglion cell axons in Xenopus tropicalis (Choi et al., pp. 846-854).

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