Sung-Hwan Moon" /> Sung-Hwan Moon, Sung-Whan Kim, Jong Soo Kim, Soon-Jung Park, Jeong Tae Do, Dong Ryul Lee, and Hyung-Min Chung*" /> Sung-Hwan Moon, Sung-Whan Kim, Jong Soo Kim, Soon-Jung Park, Jeong Tae Do, Dong Ryul Lee, and Hyung-Min Chung*. Mol. Cells 2011;31:315-26. https://doi.org/10.1007/s10059-011-0039-1">
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Mol. Cells 2011; 31(4): 315-326

Published online April 30, 2011

https://doi.org/10.1007/s10059-011-0039-1

© The Korean Society for Molecular and Cellular Biology

Gene Expression Profiles in CHA3 and CHA4 Human Embryonic Stem Cells and Embryoid Bod-ies

Sung-Hwan Moon1,6, Sung-Whan Kim2,6, Jong Soo Kim3, Soon-Jung Park4, Jeong Tae Do3, Dong Ryul Lee5, and Hyung-Min Chung1,4,*

1CHA Bio & Diostech Co., Ltd., Seoul 135-081, Korea, 2Regional Clinical Trial Center, Department of Cardiology, College of Medicine, Dong-A University, Busan 602-715, Korea, 3Laboratory of Stem Cell and Developmental Biology, CHA Stem Cell Institute, CHA University, Seoul 135-907, Korea, 4Stemcell Research Laboratory, Department of Developmental Biology, CHA University, Seoul 135-907, Korea, 5Fertility Center, CHA Gangnam Medical Center, College of Medicine, CHA University, Seoul 135-081, Korea, 6These authors contributed equally to this work.

Correspondence to : *Correspondence: stemchung@gmail.com

Received: July 19, 2011; Revised: December 31, 2011; Accepted: January 4, 2011

Abstract

The establishment of the first human embryonic stem cells (hESCs) in 1998 provided a unique tool for studying human development. Although several Western embryo-derived hESC lines are well characterized, the biological properties of Asian embryo-derived hESC lines remain unexamined. The aim of this study was to characterize Korean embryo-derived hESC lines and their differentia-tion potential. In this context, we conducted microarray-based differential gene expression analyses using two Korean embryo-derived hESC lines (CHA3 and CHA4) to identify undifferentiated and spontaneously dif-ferentiated (human embryoid body, or hEB) status. These two cell lines showed great similarity in gene expression. By comparing their expression patterns, we determined novel hESC-specific genes and transcriptomes that could serve as reliable hESC markers associated with the “stemness” phenotype. Additionally, we sought to identify hEB markers that could be used to determine the presence of differentiated cells in specific tissues, allowing for the purification of homogeneous cell populations or serving as indicators of hESC differentiation. Novel sets of 68 hESC-speci-fic markers, 12 hESC-specific transcripts and 36 hEB markers were identified and shown by quantitative RT-PCR to be similarly expressed in CHA3- and CHA4-hESC lines, as compared to the Western embryo-derived H9-hESC line. Furthermore, our data analysis revealed that the cell cycle, urea cycle, p53 signaling, and metabolism of amino groups are significantly implicated in the regulation of hESC differentiation. These results provide another unique set of hESC/hEB markers and foster a better understanding of the molecular mechanisms underlying hESC biology. These results may thus facilitate studies of human developmental events and provide information regarding Korean embryo-derived hESCs, which could be used to determine differences in developmental events between human races.

Keywords differentiation, embryoid bodies, human embryonic stem cells, microarray, transcripts

Article

Research Article

Mol. Cells 2011; 31(4): 315-326

Published online April 30, 2011 https://doi.org/10.1007/s10059-011-0039-1

Copyright © The Korean Society for Molecular and Cellular Biology.

Gene Expression Profiles in CHA3 and CHA4 Human Embryonic Stem Cells and Embryoid Bod-ies

Sung-Hwan Moon1,6, Sung-Whan Kim2,6, Jong Soo Kim3, Soon-Jung Park4, Jeong Tae Do3, Dong Ryul Lee5, and Hyung-Min Chung1,4,*

1CHA Bio & Diostech Co., Ltd., Seoul 135-081, Korea, 2Regional Clinical Trial Center, Department of Cardiology, College of Medicine, Dong-A University, Busan 602-715, Korea, 3Laboratory of Stem Cell and Developmental Biology, CHA Stem Cell Institute, CHA University, Seoul 135-907, Korea, 4Stemcell Research Laboratory, Department of Developmental Biology, CHA University, Seoul 135-907, Korea, 5Fertility Center, CHA Gangnam Medical Center, College of Medicine, CHA University, Seoul 135-081, Korea, 6These authors contributed equally to this work.

Correspondence to:*Correspondence: stemchung@gmail.com

Received: July 19, 2011; Revised: December 31, 2011; Accepted: January 4, 2011

Abstract

The establishment of the first human embryonic stem cells (hESCs) in 1998 provided a unique tool for studying human development. Although several Western embryo-derived hESC lines are well characterized, the biological properties of Asian embryo-derived hESC lines remain unexamined. The aim of this study was to characterize Korean embryo-derived hESC lines and their differentia-tion potential. In this context, we conducted microarray-based differential gene expression analyses using two Korean embryo-derived hESC lines (CHA3 and CHA4) to identify undifferentiated and spontaneously dif-ferentiated (human embryoid body, or hEB) status. These two cell lines showed great similarity in gene expression. By comparing their expression patterns, we determined novel hESC-specific genes and transcriptomes that could serve as reliable hESC markers associated with the “stemness” phenotype. Additionally, we sought to identify hEB markers that could be used to determine the presence of differentiated cells in specific tissues, allowing for the purification of homogeneous cell populations or serving as indicators of hESC differentiation. Novel sets of 68 hESC-speci-fic markers, 12 hESC-specific transcripts and 36 hEB markers were identified and shown by quantitative RT-PCR to be similarly expressed in CHA3- and CHA4-hESC lines, as compared to the Western embryo-derived H9-hESC line. Furthermore, our data analysis revealed that the cell cycle, urea cycle, p53 signaling, and metabolism of amino groups are significantly implicated in the regulation of hESC differentiation. These results provide another unique set of hESC/hEB markers and foster a better understanding of the molecular mechanisms underlying hESC biology. These results may thus facilitate studies of human developmental events and provide information regarding Korean embryo-derived hESCs, which could be used to determine differences in developmental events between human races.

Keywords: differentiation, embryoid bodies, human embryonic stem cells, microarray, transcripts

Mol. Cells
Sep 30, 2022 Vol.45 No.9, pp. 603~672
COVER PICTURE
The Target of Rapamycin Complex (TORC) is a central regulatory hub in eukaryotes, which is well conserved in diverse plant species, including tomato (Solanum lycopersicum). Inhibition of TORC genes (SlTOR, SlLST8, and SlRAPTOR) by VIGS (virus-induced gene silencing) results in early fruit ripening in tomato. The red/ orange tomatoes are early-ripened TORC-silenced fruits, while the green tomato is a control fruit. Top, left, control fruit (TRV2-myc); top, right, TRV2-SlLST8; bottom, left, TRV2-SlTOR; bottom, right, TRV2-SlRAPTOR(Choi et al., pp. 660-672).

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