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Mol. Cells 2011; 31(3): 281-287

Published online February 10, 2011

https://doi.org/10.1007/s10059-011-0035-5

© The Korean Society for Molecular and Cellular Biology

A Genetic Screen for Leaf Movement Mutants Identifies a Potential Role for AGAMOUS-LIKE 6 (AGL6) in Circadian-Clock Control

Seung Kwan Yoo1,3, Sung Myun Hong1,3, Jong Seob Lee2, and Ji Hoon Ahn1,*

1Creative Research Initiatives, School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea, 2School of Biological Sciences, Seoul National University, Seoul 151-742, Korea, 3These authors contributed equally to this work.

Correspondence to : *Correspondence: jahn@korea.ac.kr

Received: December 7, 2010; Accepted: December 21, 2010

Abstract

The circadian clock in plants regulates many important physiological and biological processes, including leaf movement. We have used an imaging system to genetically screen Arabidopsis seedlings for altered leaf movement with the aim of identifying a circadian clock gene. A total of 285 genes were selected from publicly available microarrays that showed an expression pattern similar to those of the Arabidopsis core oscillator genes. We subsequently isolated 42 homozygous recessive mutants and analyzed their leaf movements. We also analyzed leaf movements of activation tagging mutants that showed altered flowering time. We found that agl6-1D plants, in which AGAMOUS-LIKE 6 (AGL6) was activated by the 35S enhancer, showed a shortened period of leaf movement as well as a high level of ZEITLUPE (ZTL) expression, redu-ced amplitude of LATE ELONGATED HYPOCOTYL (LHY) expression, and arrhythmic TIMING OF CAB EXPRES-SION1 (TOC1)/CIRCADIAN CLOCK ASSOCIATED1 (CCA1) ex-pression. A shortened period of leaf movement was also seen in 35S-AGL6-myc plants, although 35S-amiRAGL6 plants, transgenic plants overexpressing an artificial miRNA (amiR) targeting AGL6, showed unaltered leaf movement. The amplitude of CHLOROPHYLL A/B BINDING PROTEIN 2 (CAB2) expression, a circadian output gene, was also reduced in agl6-1D plants. Taken together, these results suggest that AGL6 plays a potential role in the regulation of the circadian clock by regulating ZTL mRNA level in Arabidopsis.

Keywords AGL6, CCA1, circadian clock, leaf movement, ZTL

Article

Research Article

Mol. Cells 2011; 31(3): 281-287

Published online March 31, 2011 https://doi.org/10.1007/s10059-011-0035-5

Copyright © The Korean Society for Molecular and Cellular Biology.

A Genetic Screen for Leaf Movement Mutants Identifies a Potential Role for AGAMOUS-LIKE 6 (AGL6) in Circadian-Clock Control

Seung Kwan Yoo1,3, Sung Myun Hong1,3, Jong Seob Lee2, and Ji Hoon Ahn1,*

1Creative Research Initiatives, School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea, 2School of Biological Sciences, Seoul National University, Seoul 151-742, Korea, 3These authors contributed equally to this work.

Correspondence to:*Correspondence: jahn@korea.ac.kr

Received: December 7, 2010; Accepted: December 21, 2010

Abstract

The circadian clock in plants regulates many important physiological and biological processes, including leaf movement. We have used an imaging system to genetically screen Arabidopsis seedlings for altered leaf movement with the aim of identifying a circadian clock gene. A total of 285 genes were selected from publicly available microarrays that showed an expression pattern similar to those of the Arabidopsis core oscillator genes. We subsequently isolated 42 homozygous recessive mutants and analyzed their leaf movements. We also analyzed leaf movements of activation tagging mutants that showed altered flowering time. We found that agl6-1D plants, in which AGAMOUS-LIKE 6 (AGL6) was activated by the 35S enhancer, showed a shortened period of leaf movement as well as a high level of ZEITLUPE (ZTL) expression, redu-ced amplitude of LATE ELONGATED HYPOCOTYL (LHY) expression, and arrhythmic TIMING OF CAB EXPRES-SION1 (TOC1)/CIRCADIAN CLOCK ASSOCIATED1 (CCA1) ex-pression. A shortened period of leaf movement was also seen in 35S-AGL6-myc plants, although 35S-amiRAGL6 plants, transgenic plants overexpressing an artificial miRNA (amiR) targeting AGL6, showed unaltered leaf movement. The amplitude of CHLOROPHYLL A/B BINDING PROTEIN 2 (CAB2) expression, a circadian output gene, was also reduced in agl6-1D plants. Taken together, these results suggest that AGL6 plays a potential role in the regulation of the circadian clock by regulating ZTL mRNA level in Arabidopsis.

Keywords: AGL6, CCA1, circadian clock, leaf movement, ZTL

Mol. Cells
Jan 31, 2023 Vol.46 No.1, pp. 1~67
COVER PICTURE
RNAs form diverse shapes and play multiple functions as central molecules of gene expression. In this special issue on RNA, seven minireviews illustrate how basic concepts and recent RNA biology findings are transformed into new and exciting RNA therapeutics.

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