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Mol. Cells 2011; 31(3): 247-253

Published online January 21, 2011

https://doi.org/10.1007/s10059-011-0033-7

© The Korean Society for Molecular and Cellular Biology

Identification and Characterization of RNF2 Response Elements in Human Kidney Cells

Dongwon Choi, and Seongman Kang*

Author Info. +

Graduate School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea

Correspondence to : *Correspondence: skang@korea.ac.kr

Received: September 10, 2010; Revised: December 1, 2010; Accepted: December 17, 2010

Abstract

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RING finger protein 2 (RNF2) contains a conserved N-terminal RING finger domain and functions as an E3 ligase. As a member of the Polycomb group family of proteins, RNF2 also represses a number of genes involved in development, differentiation, malignant transformation and cell cycle. Herein, using chromatin immunoprecipitation cloning, 33 RNF2-responding loci were identified in the genome of HEK293 human kidney cells. Luciferase reporter assays showed that among them, 26 and 2 loci acted as a repressor and an activator, respectively. RNA interference revealed that the identified RNF2-responding sequences regulated the transcriptional activity of nearby promoters. This study may contribute to elucidating the mechanism underlying RNF2-mediated transcriptional regulation.

Keywords PcG complex, RNF2, RNF2-responding loci

Article

Research Article

Mol. Cells 2011; 31(3): 247-253

Published online March 31, 2011 https://doi.org/10.1007/s10059-011-0033-7

Copyright © The Korean Society for Molecular and Cellular Biology.

Identification and Characterization of RNF2 Response Elements in Human Kidney Cells

Dongwon Choi, and Seongman Kang*

Graduate School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea

Correspondence to:*Correspondence: skang@korea.ac.kr

Received: September 10, 2010; Revised: December 1, 2010; Accepted: December 17, 2010

Abstract

RING finger protein 2 (RNF2) contains a conserved N-terminal RING finger domain and functions as an E3 ligase. As a member of the Polycomb group family of proteins, RNF2 also represses a number of genes involved in development, differentiation, malignant transformation and cell cycle. Herein, using chromatin immunoprecipitation cloning, 33 RNF2-responding loci were identified in the genome of HEK293 human kidney cells. Luciferase reporter assays showed that among them, 26 and 2 loci acted as a repressor and an activator, respectively. RNA interference revealed that the identified RNF2-responding sequences regulated the transcriptional activity of nearby promoters. This study may contribute to elucidating the mechanism underlying RNF2-mediated transcriptional regulation.

Keywords: PcG complex, RNF2, RNF2-responding loci

Mol. Cells
Sep 30, 2023 Vol.46 No.9, pp. 527~572
COVER PICTURE
Chronic obstructive pulmonary disease (COPD) is marked by airspace enlargement (emphysema) and small airway fibrosis, leading to airflow obstruction and eventual respiratory failure. Shown is a microphotograph of hematoxylin and eosin (H&E)-stained histological sections of the enlarged alveoli as an indicator of emphysema. Piao et al. (pp. 558-572) demonstrate that recombinant human hyaluronan and proteoglycan link protein 1 (rhHAPLN1) significantly reduces the extended airspaces of the emphysematous alveoli by increasing the levels of TGF-β receptor I and SIRT1/6, as a previously unrecognized mechanism in human alveolar epithelial cells, and consequently mitigates COPD.
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