Mol. Cells 2011; 31(2): 191-197
Published online December 22, 2011
https://doi.org/10.1007/s10059-011-0021-y
© The Korean Society for Molecular and Cellular Biology
Correspondence to : *Correspondence: ikawasak@mac.com (IK); yshim@konkuk.ac.kr (YHS)
IFE-1 is one of the five C. elegans homologs of eIF4E, which is the mRNA 5' cap-binding component of the translation initiation complex eIF4F. Depletion of IFE-1 causes defects in sperm, suggesting that IFE-1 regulates a subset of genes required for sperm functions. To further understand the molecular function of IFE-1, proteomic analysis was performed to search for sperm proteins that are down-regulated in ife-1(ok1978); fem-3(q20) mutants relative to the fem-3(q20) control. The fem-3(q20) mutant background was used because it only produces sperm at restrictive temperature. Total worm proteins were subjected to 2D-DIGE, and differentially expressed protein spots were further identified by MALDI-TOF mass spectrometry. Among the identified proteins, GSP-3 and Major Sperm Proteins (MSPs) were found to be significantly down-regulated in the ife-1(ok1978) mutant. Moreover, RNAi of gsp-3 caused an ife-1-like phenotype. These results suggest that IFE-1 is required for efficient expression of some sperm-specific proteins, and the fertilization defect of ife-1 mutant is caused mainly by a reduced level of GSP-3.
Keywords Caenorhabditis elegans, eIF4E, IFE-1, proteomic analysis, sperm
Mol. Cells 2011; 31(2): 191-197
Published online February 28, 2011 https://doi.org/10.1007/s10059-011-0021-y
Copyright © The Korean Society for Molecular and Cellular Biology.
Ichiro Kawasaki*, Myung-Hwan Jeong, and Yhong-Hee Shim*
Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Korea
Correspondence to:*Correspondence: ikawasak@mac.com (IK); yshim@konkuk.ac.kr (YHS)
IFE-1 is one of the five C. elegans homologs of eIF4E, which is the mRNA 5' cap-binding component of the translation initiation complex eIF4F. Depletion of IFE-1 causes defects in sperm, suggesting that IFE-1 regulates a subset of genes required for sperm functions. To further understand the molecular function of IFE-1, proteomic analysis was performed to search for sperm proteins that are down-regulated in ife-1(ok1978); fem-3(q20) mutants relative to the fem-3(q20) control. The fem-3(q20) mutant background was used because it only produces sperm at restrictive temperature. Total worm proteins were subjected to 2D-DIGE, and differentially expressed protein spots were further identified by MALDI-TOF mass spectrometry. Among the identified proteins, GSP-3 and Major Sperm Proteins (MSPs) were found to be significantly down-regulated in the ife-1(ok1978) mutant. Moreover, RNAi of gsp-3 caused an ife-1-like phenotype. These results suggest that IFE-1 is required for efficient expression of some sperm-specific proteins, and the fertilization defect of ife-1 mutant is caused mainly by a reduced level of GSP-3.
Keywords: Caenorhabditis elegans, eIF4E, IFE-1, proteomic analysis, sperm
Mohammad Al-Amin, Ichiro Kawasaki, Joomi Gong, and Yhong-Hee Shim
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