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Mol. Cells 2011; 31(2): 165-173

Published online December 22, 2011

https://doi.org/10.1007/s10059-011-0022-x

© The Korean Society for Molecular and Cellular Biology

Immunostimulating Activity by Polysaccharides Isolated from Fruiting Body of Inonotus obliquus

Dong Pil Won, Jong Seok Lee, Duck Soo Kwon, Keun Eok Lee, Won Cheol Shin, and Eock Kee Hong*

Department of Bioengineering and Technology, Kangwon National University, Chuncheon 200-701, Korea

Correspondence to : *Correspondence: ekhong@kangwon.ac.kr

Received: September 15, 2010; Revised: October 27, 2010; Accepted: November 23, 2010

Abstract

In this study, we investigated the immunostimulating activity of polysaccharides isolated from fruiting body of Inonotus obliquus (PFIO). Additionally, the signaling pathway of PFIO-mediated macrophage activation was investigated in RAW264.7 macrophage cells. We found that PFIO was capable of promoting NO/ROS production, TNF-α secretion and phagocytic uptake in macrophages, as well as cell proliferation, comitogenic effect and IFN-γ/IL-4 secretion in mouse splenocytes. PFIO was able to induce the phosphorylation of three MAPKs as well as the nuclear translocation of NF-κB, resulting in activation of RAW264.7 macrophages. PFIO also induced the inhibition of TNF-α secretion by anti-TLR2 mAb, consequently, PFIO might be involved in TNF-α secretion via the TLR2 receptor. In addition, our results showed that oral administration of PFIO suppressed in vivo growth of melanoma tumor in tumor-bearing mice. In conclusion, our experiments presented that PFIO effectively promotes macrophage activation through the MAPK and NF-κB signaling pathways, suggesting that PFIO may potentially regulate the immune response.

Keywords immunostimulating activity, Inonotus obliquus, MAPKs, NF-κB, pattern-recognition receptor

Article

Research Article

Mol. Cells 2011; 31(2): 165-173

Published online February 28, 2011 https://doi.org/10.1007/s10059-011-0022-x

Copyright © The Korean Society for Molecular and Cellular Biology.

Immunostimulating Activity by Polysaccharides Isolated from Fruiting Body of Inonotus obliquus

Dong Pil Won, Jong Seok Lee, Duck Soo Kwon, Keun Eok Lee, Won Cheol Shin, and Eock Kee Hong*

Department of Bioengineering and Technology, Kangwon National University, Chuncheon 200-701, Korea

Correspondence to:*Correspondence: ekhong@kangwon.ac.kr

Received: September 15, 2010; Revised: October 27, 2010; Accepted: November 23, 2010

Abstract

In this study, we investigated the immunostimulating activity of polysaccharides isolated from fruiting body of Inonotus obliquus (PFIO). Additionally, the signaling pathway of PFIO-mediated macrophage activation was investigated in RAW264.7 macrophage cells. We found that PFIO was capable of promoting NO/ROS production, TNF-α secretion and phagocytic uptake in macrophages, as well as cell proliferation, comitogenic effect and IFN-γ/IL-4 secretion in mouse splenocytes. PFIO was able to induce the phosphorylation of three MAPKs as well as the nuclear translocation of NF-κB, resulting in activation of RAW264.7 macrophages. PFIO also induced the inhibition of TNF-α secretion by anti-TLR2 mAb, consequently, PFIO might be involved in TNF-α secretion via the TLR2 receptor. In addition, our results showed that oral administration of PFIO suppressed in vivo growth of melanoma tumor in tumor-bearing mice. In conclusion, our experiments presented that PFIO effectively promotes macrophage activation through the MAPK and NF-κB signaling pathways, suggesting that PFIO may potentially regulate the immune response.

Keywords: immunostimulating activity, Inonotus obliquus, MAPKs, NF-κB, pattern-recognition receptor

Mol. Cells
Jan 31, 2023 Vol.46 No.1, pp. 1~67
COVER PICTURE
RNAs form diverse shapes and play multiple functions as central molecules of gene expression. In this special issue on RNA, seven minireviews illustrate how basic concepts and recent RNA biology findings are transformed into new and exciting RNA therapeutics.

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