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Mol. Cells 2011; 31(1): 25-31

Published online November 23, 2010

https://doi.org/10.1007/s10059-011-0004-z

© The Korean Society for Molecular and Cellular Biology

The RNase Activity of Rice Probenazole-Induced Protein1 (PBZ1) Plays a Key Role in Cell Death in Plants

Sang Gon Kim1,9, Sun Tae Kim2,9, Yiming Wang3, Seok Yu1, In Soo Choi2, Yong Chul Kim2, Woo Taek Kim5, Ganesh Kumar Agrawal6, Randeep Rakwal6,7,8, and Kyu Young Kang1,3,4,*

1Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660-701, Korea, 2Department of Plant Bioscience, Pusan National University, Busan 609-735, Korea, 3Division of Applied Life Science (Brain Korea 21 Program), Gyeongsang National University, Jinju 660-701, Korea, 4Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Korea, 5Department of Biology, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Korea, 6Research Laboratory for Biotechnology and Biochemistry (RLABB), GPO Box 13265, Kathmandu, Nepal, 7Showa University, School of Medicine, Shinagawa, Tokyo 142-8555, Japan, 8Toho University, Faculty of Science, Funabashi, Chiba 274-8510, Japan, 9These authors contributed equally to this work.

Correspondence to : *Correspondence: kykang@gnu.ac.kr

Received: June 4, 2010; Revised: September 9, 2010; Accepted: October 12, 2010

Abstract

Cell death is an important process of plant responses to development and biotic/abiotic stresses. In rice plants, PBZ1, a PR10 family protein, has been shown to accumu-late in tissues undergoing cell death. However, the func-tion of PBZ1 in cell death remains yet to be demonstrated. Here, we report that exogenous recombinant PBZ1 protein induces cell death in rice suspension-cultured cells (SCCs) and also in leaves of Nicotiana tabacum in a dose-dependent manner. This finding was confirmed in vivo in transgenic Arabidopsis lines harboring the PBZ1 gene under the control of a dexamethasone (DEX)-inducible promoter. The DEX-treated leaves of transgenic Arabidopsis induced expression of PBZ1 at transcript and protein levels and showed cell death morphology. TUNEL analysis detected DNA fragmentation, a hallmark of programmed cell death, in rice SCCs treated with the PBZ1 protein. Recombinant PBZ1 protein also exhibited RNase activity and exhibited internalization inside BY-2 cells. Taken together, PBZ1 induces cell death not only in rice, but also in tobacco and Arabidopsis via its RNase activity inside the cell. PBZ1 could be used as a marker to understand the me-chanism by which PBZ1 confers the cell death morpholo-gy in rice and other model plants.

Keywords dexamethasone, PBZ1, PR-10 protein family, programmed cell death, RNase activity, TUNEL

Article

Research Article

Mol. Cells 2011; 31(1): 25-31

Published online January 31, 2011 https://doi.org/10.1007/s10059-011-0004-z

Copyright © The Korean Society for Molecular and Cellular Biology.

The RNase Activity of Rice Probenazole-Induced Protein1 (PBZ1) Plays a Key Role in Cell Death in Plants

Sang Gon Kim1,9, Sun Tae Kim2,9, Yiming Wang3, Seok Yu1, In Soo Choi2, Yong Chul Kim2, Woo Taek Kim5, Ganesh Kumar Agrawal6, Randeep Rakwal6,7,8, and Kyu Young Kang1,3,4,*

1Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660-701, Korea, 2Department of Plant Bioscience, Pusan National University, Busan 609-735, Korea, 3Division of Applied Life Science (Brain Korea 21 Program), Gyeongsang National University, Jinju 660-701, Korea, 4Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Korea, 5Department of Biology, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Korea, 6Research Laboratory for Biotechnology and Biochemistry (RLABB), GPO Box 13265, Kathmandu, Nepal, 7Showa University, School of Medicine, Shinagawa, Tokyo 142-8555, Japan, 8Toho University, Faculty of Science, Funabashi, Chiba 274-8510, Japan, 9These authors contributed equally to this work.

Correspondence to:*Correspondence: kykang@gnu.ac.kr

Received: June 4, 2010; Revised: September 9, 2010; Accepted: October 12, 2010

Abstract

Cell death is an important process of plant responses to development and biotic/abiotic stresses. In rice plants, PBZ1, a PR10 family protein, has been shown to accumu-late in tissues undergoing cell death. However, the func-tion of PBZ1 in cell death remains yet to be demonstrated. Here, we report that exogenous recombinant PBZ1 protein induces cell death in rice suspension-cultured cells (SCCs) and also in leaves of Nicotiana tabacum in a dose-dependent manner. This finding was confirmed in vivo in transgenic Arabidopsis lines harboring the PBZ1 gene under the control of a dexamethasone (DEX)-inducible promoter. The DEX-treated leaves of transgenic Arabidopsis induced expression of PBZ1 at transcript and protein levels and showed cell death morphology. TUNEL analysis detected DNA fragmentation, a hallmark of programmed cell death, in rice SCCs treated with the PBZ1 protein. Recombinant PBZ1 protein also exhibited RNase activity and exhibited internalization inside BY-2 cells. Taken together, PBZ1 induces cell death not only in rice, but also in tobacco and Arabidopsis via its RNase activity inside the cell. PBZ1 could be used as a marker to understand the me-chanism by which PBZ1 confers the cell death morpholo-gy in rice and other model plants.

Keywords: dexamethasone, PBZ1, PR-10 protein family, programmed cell death, RNase activity, TUNEL

Mol. Cells
Nov 30, 2023 Vol.46 No.11, pp. 655~725
COVER PICTURE
Kim et al. (pp. 710-724) demonstrated that a pathogen-derived Ralstonia pseudosolanacearum type III effector RipL delays flowering time and enhances susceptibility to bacterial infection in Arabidopsis thaliana. Shown is the RipL-expressing Arabidopsis plant, which displays general dampening of the transcriptional program during pathogen infection, grown in long-day conditions.

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