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Mol. Cells 2010; 30(6): 563-567

Published online December 31, 2010

https://doi.org/10.1007/s10059-010-0153-5

© The Korean Society for Molecular and Cellular Biology

Chemoattractant-Mediated Rap1 Activation Requires GPCR/G Proteins

Injun Cha, Sung H. Lee1, and Taeck J. Jeon*

Department of Biology, College of Natural Sciences, Chosun University, Gwangju 501-759, Korea, 1Department of Cellular and Molecular Medicine, School of medicine, Chosun University, Gwangju 501-759, Korea

Correspondence to : *Correspondence: tjeon@chosun.ac.kr

Received: August 10, 2010; Revised: September 8, 2010; Accepted: September 10, 2010

Abstract

Rap1 is rapidly activated upon chemoattractant stimula-tion and plays an important role in cell adhesion and cytoskeletal reorganization during chemotaxis. Here, we de-monstrate that G-protein coupled receptors and G-proteins are essential for chemoattractant-mediated Rap1 activation in Dictyostelium. The rapid Rap1 activation upon cAMP chemoattractant stimulation was absent in cells lacking chemoattractant cAMP receptors cAR1/cAR3 or a subunit of the heterotrimeric G-protein complex Gα2. Loss of guanylyl cyclases GCA/SGC or a cGMP-binding protein GbpC exhibited no effect on Rap1 activation kinetics. These results suggest that Rap1, a key regulator for the regulation of cytoskeletal reorganization during cell move-ment, is activated through the G-protein coupled receptors cAR1/cAR3 and Gα2 proteins in a way independent of the cGMP signaling pathway.

Keywords chemotaxis, Dictyostelium, GPCR, Rap1, signal transduction

Article

Research Article

Mol. Cells 2010; 30(6): 563-567

Published online December 31, 2010 https://doi.org/10.1007/s10059-010-0153-5

Copyright © The Korean Society for Molecular and Cellular Biology.

Chemoattractant-Mediated Rap1 Activation Requires GPCR/G Proteins

Injun Cha, Sung H. Lee1, and Taeck J. Jeon*

Department of Biology, College of Natural Sciences, Chosun University, Gwangju 501-759, Korea, 1Department of Cellular and Molecular Medicine, School of medicine, Chosun University, Gwangju 501-759, Korea

Correspondence to:*Correspondence: tjeon@chosun.ac.kr

Received: August 10, 2010; Revised: September 8, 2010; Accepted: September 10, 2010

Abstract

Rap1 is rapidly activated upon chemoattractant stimula-tion and plays an important role in cell adhesion and cytoskeletal reorganization during chemotaxis. Here, we de-monstrate that G-protein coupled receptors and G-proteins are essential for chemoattractant-mediated Rap1 activation in Dictyostelium. The rapid Rap1 activation upon cAMP chemoattractant stimulation was absent in cells lacking chemoattractant cAMP receptors cAR1/cAR3 or a subunit of the heterotrimeric G-protein complex Gα2. Loss of guanylyl cyclases GCA/SGC or a cGMP-binding protein GbpC exhibited no effect on Rap1 activation kinetics. These results suggest that Rap1, a key regulator for the regulation of cytoskeletal reorganization during cell move-ment, is activated through the G-protein coupled receptors cAR1/cAR3 and Gα2 proteins in a way independent of the cGMP signaling pathway.

Keywords: chemotaxis, Dictyostelium, GPCR, Rap1, signal transduction

Mol. Cells
Sep 30, 2022 Vol.45 No.9, pp. 603~672
COVER PICTURE
The Target of Rapamycin Complex (TORC) is a central regulatory hub in eukaryotes, which is well conserved in diverse plant species, including tomato (Solanum lycopersicum). Inhibition of TORC genes (SlTOR, SlLST8, and SlRAPTOR) by VIGS (virus-induced gene silencing) results in early fruit ripening in tomato. The red/ orange tomatoes are early-ripened TORC-silenced fruits, while the green tomato is a control fruit. Top, left, control fruit (TRV2-myc); top, right, TRV2-SlLST8; bottom, left, TRV2-SlTOR; bottom, right, TRV2-SlRAPTOR(Choi et al., pp. 660-672).

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