Top

Research Article

Split Viewer

Mol. Cells 2010; 30(5): 449-454

Published online November 30, 2010

https://doi.org/10.1007/s10059-010-0139-3

© The Korean Society for Molecular and Cellular Biology

Wnt/β-Catenin Signaling Enhances Osteoblastogenic Differentiation from Human Periodontal Ligament Fibroblasts

Jung Sun Heo1, Seung-Youp Lee2, and Jeong-Chae Lee3,4,*

1Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul 130-701, Korea, 2Department of Dentistry, Gangneung-Wonju National University, Gangwon 210-702, Korea, 3Institute of Oral Biosciences and School of Dentistry (Brain Korea 21 Program), Chonbuk National University, Jeonju 561-756, Korea, 4Department of Bioactive Material Sciences and Research Center of Bioactive Materials, Chonbuk National University, Jeonju 561-756, Korea

Correspondence to : *Correspondence: leejc88@jbnu.ac.kr

Received: June 1, 2010; Revised: August 10, 2010; Accepted: August 19, 2010

Abstract

Wnt/β-catenin signaling has been known to influence bone formation and homeostasis. In this study, we inves-tigated the canonical Wnt signaling regulation of osteogenic differentiation from periodontal ligament (PDL) fibroblasts. Stimulating PDL fibroblasts with lithium chloride (LiCl), a canonical Wnt activator, significantly increased mineralized nodule and alkaline phosphatase (ALP) activity in a time- and dose-dependent manner. LiCl up-regulated protein expression of osteogenic transcription factors, including the runt-related gene 2, Msx2, and Osterix 2, in the PDL fibroblasts. Treatment of these cells with LiCl also in-creased the mRNA levels of ALP, FosB, and Fra1 in a dose-dependent manner. Blockage of canonical Wnt sig-naling by treating the cells with DKK1 inhibited Wnt1-stimulated mRNA expression of these osteogenic factors. Furthermore, pretreatment with DKK1 reduced the ALP activity and matrix mineralization stimulated by Wnt1. Collectively, these results suggest that canonical Wnt signaling leads to the differentiation of PDL fibroblasts into osteogenic lineage with the attendant stimulation of osteogenic transcription factors.

Keywords LiCl, osteoblastogenesis, osteogenic transcription factors, PDL fibroblasts, Wnt/

Article

Research Article

Mol. Cells 2010; 30(5): 449-454

Published online November 30, 2010 https://doi.org/10.1007/s10059-010-0139-3

Copyright © The Korean Society for Molecular and Cellular Biology.

Wnt/β-Catenin Signaling Enhances Osteoblastogenic Differentiation from Human Periodontal Ligament Fibroblasts

Jung Sun Heo1, Seung-Youp Lee2, and Jeong-Chae Lee3,4,*

1Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul 130-701, Korea, 2Department of Dentistry, Gangneung-Wonju National University, Gangwon 210-702, Korea, 3Institute of Oral Biosciences and School of Dentistry (Brain Korea 21 Program), Chonbuk National University, Jeonju 561-756, Korea, 4Department of Bioactive Material Sciences and Research Center of Bioactive Materials, Chonbuk National University, Jeonju 561-756, Korea

Correspondence to:*Correspondence: leejc88@jbnu.ac.kr

Received: June 1, 2010; Revised: August 10, 2010; Accepted: August 19, 2010

Abstract

Wnt/β-catenin signaling has been known to influence bone formation and homeostasis. In this study, we inves-tigated the canonical Wnt signaling regulation of osteogenic differentiation from periodontal ligament (PDL) fibroblasts. Stimulating PDL fibroblasts with lithium chloride (LiCl), a canonical Wnt activator, significantly increased mineralized nodule and alkaline phosphatase (ALP) activity in a time- and dose-dependent manner. LiCl up-regulated protein expression of osteogenic transcription factors, including the runt-related gene 2, Msx2, and Osterix 2, in the PDL fibroblasts. Treatment of these cells with LiCl also in-creased the mRNA levels of ALP, FosB, and Fra1 in a dose-dependent manner. Blockage of canonical Wnt sig-naling by treating the cells with DKK1 inhibited Wnt1-stimulated mRNA expression of these osteogenic factors. Furthermore, pretreatment with DKK1 reduced the ALP activity and matrix mineralization stimulated by Wnt1. Collectively, these results suggest that canonical Wnt signaling leads to the differentiation of PDL fibroblasts into osteogenic lineage with the attendant stimulation of osteogenic transcription factors.

Keywords: LiCl, osteoblastogenesis, osteogenic transcription factors, PDL fibroblasts, Wnt/

Mol. Cells
Sep 30, 2022 Vol.45 No.9, pp. 603~672
COVER PICTURE
The Target of Rapamycin Complex (TORC) is a central regulatory hub in eukaryotes, which is well conserved in diverse plant species, including tomato (Solanum lycopersicum). Inhibition of TORC genes (SlTOR, SlLST8, and SlRAPTOR) by VIGS (virus-induced gene silencing) results in early fruit ripening in tomato. The red/ orange tomatoes are early-ripened TORC-silenced fruits, while the green tomato is a control fruit. Top, left, control fruit (TRV2-myc); top, right, TRV2-SlLST8; bottom, left, TRV2-SlTOR; bottom, right, TRV2-SlRAPTOR(Choi et al., pp. 660-672).

Share this article on

  • line
  • mail

Related articles in Mol. Cells

Molecules and Cells

eISSN 0219-1032
qr-code Download