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Mol. Cells 2010; 30(5): 393-401

Published online November 30, 2010

https://doi.org/10.1007/s10059-010-0129-5

© The Korean Society for Molecular and Cellular Biology

Molecular Cloning, Expression and Characterization of Bovine UQCC and Its Association with Body Measurement Traits

Yongfeng Liu1,2, Linsen Zan1,3,*, Shuanping Zhao1, Honggang Huang2, Yong Li2, Zhonglin Tang2, Shulin Yang2, and Kui Li2,*

1College of Animal Science and Technology, Northwest A & F University, Yangling 712100, Shaanxi, People’s Republic of China, 2Key Laboratory for Farm Animal Genetic Resources and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, People’s Republic of China, 3National Beef cattle improvement centre of China, Yangling 712100, Shaanxi, People’s Republic of China

Correspondence to : *Correspondence: zanls@yahoo.com.cn (LZ); kuili@iascaas.net.cn (KL)

Received: December 24, 2010; Revised: July 20, 2010; Accepted: July 26, 2010

Abstract

Ubiquinol-cytochrome c reductase complex chaperone (UQCC) involved in the development and maintenance of bone and cartilage is an important candidate gene for body measurement traits selection through marker-assisted selection (MAS). The expression of UQCC is upregulated in many human and animal models of height as well as other stature indexes. We have cloned the cDNA sequence coding UQCC gene in bovine. Genomic structural analysis indicated that bovine UQCC shares a high similarity with human UQCC. Furthermore, Real-Time PCR analysis showed that the expression of bovine UQCC is remarkably different in diverse tissues, including high level expression in the spleen, heart and windpipe, and relatively low expression in other tissues. We also analyzed allele frequencies in different cattle breeds and an association study on the selected SNPs. SNP DraI A2691T in intron 1 and SNP Bsh1236I A3150G in intron 8 are significantly associated with Body Length (BL), Rump Length (RL), Chest Depth (CD) and Pin Bone Width (PBW). For the A2691T SNP marker, there are significant effects on the RL (p = 0.0001), CD (p = 0.0059) and PBW (p < 0.0001) in 679 individuals; with A3150G SNP marker, there are significant effects on the BL (p = 0.0047) and CD (p = 0.0454). Regarding association analysis of combination of the two SNPs, there are significant effects on the BL (p = 0.0215), CD (p = 0.0282) and PBW (p = 0.0329) in the total population. The results suggest that the UQCC gene is a candidate gene of body measurement traits in bovine reproduction and breeding, and provide data for establishing an animal model using cattle to study big animal body type.

Keywords : association analysis, body measurement, cattle, genomic structure, SNP polymorphism, Ubiquinol-cytochrome c reductase complex chaperone (UQCC)

Article

Research Article

Mol. Cells 2010; 30(5): 393-401

Published online November 30, 2010 https://doi.org/10.1007/s10059-010-0129-5

Copyright © The Korean Society for Molecular and Cellular Biology.

Molecular Cloning, Expression and Characterization of Bovine UQCC and Its Association with Body Measurement Traits

Yongfeng Liu1,2, Linsen Zan1,3,*, Shuanping Zhao1, Honggang Huang2, Yong Li2, Zhonglin Tang2, Shulin Yang2, and Kui Li2,*

1College of Animal Science and Technology, Northwest A & F University, Yangling 712100, Shaanxi, People’s Republic of China, 2Key Laboratory for Farm Animal Genetic Resources and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, People’s Republic of China, 3National Beef cattle improvement centre of China, Yangling 712100, Shaanxi, People’s Republic of China

Correspondence to:*Correspondence: zanls@yahoo.com.cn (LZ); kuili@iascaas.net.cn (KL)

Received: December 24, 2010; Revised: July 20, 2010; Accepted: July 26, 2010

Abstract

Ubiquinol-cytochrome c reductase complex chaperone (UQCC) involved in the development and maintenance of bone and cartilage is an important candidate gene for body measurement traits selection through marker-assisted selection (MAS). The expression of UQCC is upregulated in many human and animal models of height as well as other stature indexes. We have cloned the cDNA sequence coding UQCC gene in bovine. Genomic structural analysis indicated that bovine UQCC shares a high similarity with human UQCC. Furthermore, Real-Time PCR analysis showed that the expression of bovine UQCC is remarkably different in diverse tissues, including high level expression in the spleen, heart and windpipe, and relatively low expression in other tissues. We also analyzed allele frequencies in different cattle breeds and an association study on the selected SNPs. SNP DraI A2691T in intron 1 and SNP Bsh1236I A3150G in intron 8 are significantly associated with Body Length (BL), Rump Length (RL), Chest Depth (CD) and Pin Bone Width (PBW). For the A2691T SNP marker, there are significant effects on the RL (p = 0.0001), CD (p = 0.0059) and PBW (p < 0.0001) in 679 individuals; with A3150G SNP marker, there are significant effects on the BL (p = 0.0047) and CD (p = 0.0454). Regarding association analysis of combination of the two SNPs, there are significant effects on the BL (p = 0.0215), CD (p = 0.0282) and PBW (p = 0.0329) in the total population. The results suggest that the UQCC gene is a candidate gene of body measurement traits in bovine reproduction and breeding, and provide data for establishing an animal model using cattle to study big animal body type.

Keywords: : association analysis, body measurement, cattle, genomic structure, SNP polymorphism, Ubiquinol-cytochrome c reductase complex chaperone (UQCC)

Mol. Cells
Sep 30, 2022 Vol.45 No.9, pp. 603~672
COVER PICTURE
The Target of Rapamycin Complex (TORC) is a central regulatory hub in eukaryotes, which is well conserved in diverse plant species, including tomato (Solanum lycopersicum). Inhibition of TORC genes (SlTOR, SlLST8, and SlRAPTOR) by VIGS (virus-induced gene silencing) results in early fruit ripening in tomato. The red/ orange tomatoes are early-ripened TORC-silenced fruits, while the green tomato is a control fruit. Top, left, control fruit (TRV2-myc); top, right, TRV2-SlLST8; bottom, left, TRV2-SlTOR; bottom, right, TRV2-SlRAPTOR(Choi et al., pp. 660-672).

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