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Mol. Cells 2010; 30(3): 255-262

Published online September 30, 2010

https://doi.org/10.1007/s10059-010-0116-x

© The Korean Society for Molecular and Cellular Biology

Calcineurin Regulates Coelomocyte Endocytosis via DYN-1 and CUP-4 in Caenorhabditis elegans

Hyun-Ok Song1, Jungsoo Lee1,4, Yon Ju Ji1,5, Meenakshi Dwivedi1, Jeong Hoon Cho2, Byung-Jae Park3, and Joohong Ahnn1,*

1Department of Life Science, College of Natural Sciences, Hanyang University, Seoul 133-791, Korea, 2Division of Biology Education, College of Education, Chosun University, Gwangju 501-759, Korea, 3Department of Life Science, Hallym University, Chunchon 200-702, Korea, 4Present address: Ernest Gallo Clinic and Research Center, Department of Neurology, Programs in Neuroscience and Biomedical Science, University of California, San Francisco, California 94608, USA, 5Present address: Laboratory of Cell and Developmental Signaling, National Cancer Institute-Frederick, Maryland 21702, USA

Correspondence to : *Correspondence: joohong@hanyang.ac.kr

Received: April 28, 2010; Revised: May 30, 2010; Accepted: June 1, 2010

Abstract

C. elegans coelomocytes are macrophage-like scavenger cells that provide an excellent in vivo system for the study of clathrin-mediated endocytosis. Using this in vivo system, several genes involved in coelomocyte endocytosis have been identified previously. However, the detailed mechanism of endocytic pathway is still unknown. Here, we report a new function of calcineurin, an evolutionarily conserved Ca2+/calmodulin-dependent Ser/Thr protein phos- phatase, in coelomocyte endocytosis. We found that calcineurin mutants show defective coelomocyte endocytosis. Genetic analysis suggests that calcineurin and a GTPase, dynamin (DYN-1), may function upstream of an orphan receptor, CUP-4, to regulate endocytosis. Therefore, we propose a model in which calcineurin may regulate coelomocyte endocytosis via DYN-1 and CUP-4 in C. elegans.

Keywords Ca2+ signaling, C. elegans, endocytosis, phosphatase

Article

Research Article

Mol. Cells 2010; 30(3): 255-262

Published online September 30, 2010 https://doi.org/10.1007/s10059-010-0116-x

Copyright © The Korean Society for Molecular and Cellular Biology.

Calcineurin Regulates Coelomocyte Endocytosis via DYN-1 and CUP-4 in Caenorhabditis elegans

Hyun-Ok Song1, Jungsoo Lee1,4, Yon Ju Ji1,5, Meenakshi Dwivedi1, Jeong Hoon Cho2, Byung-Jae Park3, and Joohong Ahnn1,*

1Department of Life Science, College of Natural Sciences, Hanyang University, Seoul 133-791, Korea, 2Division of Biology Education, College of Education, Chosun University, Gwangju 501-759, Korea, 3Department of Life Science, Hallym University, Chunchon 200-702, Korea, 4Present address: Ernest Gallo Clinic and Research Center, Department of Neurology, Programs in Neuroscience and Biomedical Science, University of California, San Francisco, California 94608, USA, 5Present address: Laboratory of Cell and Developmental Signaling, National Cancer Institute-Frederick, Maryland 21702, USA

Correspondence to:*Correspondence: joohong@hanyang.ac.kr

Received: April 28, 2010; Revised: May 30, 2010; Accepted: June 1, 2010

Abstract

C. elegans coelomocytes are macrophage-like scavenger cells that provide an excellent in vivo system for the study of clathrin-mediated endocytosis. Using this in vivo system, several genes involved in coelomocyte endocytosis have been identified previously. However, the detailed mechanism of endocytic pathway is still unknown. Here, we report a new function of calcineurin, an evolutionarily conserved Ca2+/calmodulin-dependent Ser/Thr protein phos- phatase, in coelomocyte endocytosis. We found that calcineurin mutants show defective coelomocyte endocytosis. Genetic analysis suggests that calcineurin and a GTPase, dynamin (DYN-1), may function upstream of an orphan receptor, CUP-4, to regulate endocytosis. Therefore, we propose a model in which calcineurin may regulate coelomocyte endocytosis via DYN-1 and CUP-4 in C. elegans.

Keywords: Ca2+ signaling, C. elegans, endocytosis, phosphatase

Mol. Cells
Nov 30, 2022 Vol.45 No.11, pp. 763~867
COVER PICTURE
Naive (cyan) and axotomized (magenta) retinal ganglion cell axons in Xenopus tropicalis (Choi et al., pp. 846-854).

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